Amebiasis
(Amebic Dysentery)
Causal agent: Entamoeba histolytica is well recognized
as a pathogenic amoeba.
History: Loosh was first described in 1875
Geographic Distribution: Worldwide, with higher
incidence of amebiasis in developing countries.
In industrialized countries, risk groups include male
homosexuals, travelers and recent immigrants, and
institutionalized populations.
Morphology
1- trophozoite
2- precyst
3- cyst(1, 2, 4 nuclei)
Trophozoite chractere
Pseudopodia:
Motility:
Ectoplasm:
Endoplasm: may be contain ingested RBC
Nucleoplasm:
Non-invasive form
invasive form
Life cycle
Life cycle
Epidemiology
Transmisin
Pathogenesis
factores eficaces :
1- cepa virulienta:
- cepa clsica
- cepa no clsica ; Laredo , Huff, .
- sindrome patogenico
Pathogenicity mechanisms
2 muerte celular
3 citofagocitosis
Clinical symptoms
Asymptomatic infection
Symptomatic infection
Intestinal Amebiasis
Dysenteric
Non-Dysenteric colitis
Extraintestinal Amebiasis
Hepatic Pulmonary
Liver abscces
Acut nonsupprative
Pathology of Amebiasis
Flask-like Ulcer
Extra-ntestinalAmebiasis
Liver abscess
This is an amebic abscess of liver. Abscesses may arise in liver when there is seeding of
infection from the bowel, because the infectious agents are carried to the liver from the
portal venous circulation.
Diagnosis
Paraclinical Diagnosis:
Sigmoidoscopic examination:
Hepatomegally
C.B.C. : leukocytosis in Amebic dys. rises above 12000 per
microliter, but counts may reach 16000 to 20000 per microliter.
Laboratory Diagnosis
Microscopy
Microscopic identification
This can be accomplished using:
Microscopy
B
A
GHI
Immunodiagnosis
(Antibody Detection)
1- Antibody detection
Antigen Detection
Antigen detection may be useful as an adjunct to microscopic
diagnosis in detecting parasites and to distinguish between
pathogenic and nonpathogenic infections.
Recent studies indicate improved sensitivity and specificity of
fecal antigen assays with the use of monoclonal antibodies
which can distinguish between E. histolytica and E. dispar
infections.
Molecular diagnosis
Treatment
Intestinal Amebiasis:
*Asymptomatic amebiasis(cyst passer): Diloxanide furoate
( furamide)
500 mg 3 times daily / 10 days
Treatment
Extraintestinal Amebiasis: