Anda di halaman 1dari 22

The Anti inflammatory Property of Passiflora edulis (Passion fruit) Leaves

Extract and Non-steroidal Anti-inflammatory Drugs in Carrageenan-Induced


Inflammation on Male Albino Mice
Daniel C. Panaglima

Ma. Janielou V. Ocampos

Aldin S. Piamonte

Stephanie P. Ong

Candy Krista A. Pilapil

Dan Dominick B. Perral

Ceylon Quinnie E. Prayon

Introduction in brief

caring humanity

Amid the face of recent research scrutiny, P.


edulis surged to reveal different important
properties with many potential applications
including its strong anti-inflammatory
property [1].

Central to various studies is its alkaloidal


component which is believed to be the cause
of this potent effect [1][2][3][4][5][6].

SHORT OVERVIEW PG.2

Research Plant
Kingdom: PLANTAE

caring humanity

Phylum: TRACHEOPHYTA
Class: MAGNOLIOPSIDA
Order: MALPIGHIALES
Family: PASSIFLORACEAE
Genus: PASSIFLORA L.
Species: PASSIFLORA EDULIS

Common Name: PASSION FRUIT


SHORT OVERVIEW PG.3

Research Subject
Kingdom: ANIMALIA

caring humanity

Phylum: CHORDATA
Class: MAMMALIA
Order: RODENTIA
Family: MURUDAE ILLIGER
Genus: MUS LINNAEUS
Species: MUS MUSCULUS

Common Name: ALBINO MICE


SHORT OVERVIEW PG.4

Research Objective

caring humanity

General Objective:
This study aims to refine and elucidate the
effectiveness of the anti-inflammatory effect
of P. edulis leaves extract against
nonsteroidal anti- inflammatory drugs (NSAIDS)
namely aspirin, naproxen and indomethacin.

SHORT OVERVIEW PG.5

Research Objective
Specific Objectives:

caring humanity

Specifically, the study aims to:


1. Determine the mean percentage reduction of
inflammation in the treatment groups at
different time intervals post- induction:
a.

Four (4) hours

b.

Eight (8) hours

c.

Twelve (12) hours

d.

Sixteen (16) hours

e.

Twenty (20) hours

f.

Twenty- four (24) hours

SHORT OVERVIEW PG.6

Research Objective

caring humanity

2. Determine the percentage reduction of


carrageenan- induced inflammation in the
treatment groups for the whole 24 hours.
3. Determine the significant difference of
carrageenan- induced inflammation in the
treatment groups at different time intervals
between and within groups.
4. Determine the mean frequency of cases and the
degree of severity of the carrageenan- induced
inflammation using Modified Likert Scale.
SHORT OVERVIEW PG.7

Materials & Method

caring humanity

Research Design
True experimental design

Parameters
Reduction of swelling
Histopathology
SHORT OVERVIEW PG.8

Preparation of P. edulis Leaves


Extract

caring humanity
SHORT OVERVIEW PG.9

Preparation of Subjects and


Administration of Pre- induction
Test Solutions

caring humanity
SHORT OVERVIEW PG.10

Induction and Measurement of


Inflammation

caring humanity
SHORT OVERVIEW PG.11

Administration of Post- induction Test


Solutions, Measurement of
Inflammation and Skin Nip Biopsy

caring humanity
SHORT OVERVIEW PG.12

Histopathological Slides Preparation


and Reading

caring humanity
SHORT OVERVIEW PG.13

Phytochemical Analysis Result


Test

Constituent

Theoretical

Result of the Test

Interpretation

caring humanity

Positive Result
Ferric Chloride
Froth Test

Tannins

Green Precipitate

Brown Precipitate

Negative

Saponins

Stable foam,

No foam formation

Negative

honeycomb in

observed

shapes
Lead acetate
Culvenor Fitzgerald
Leibermanns

Flavonoids

Yellow Precipitate

Yellow Precipitate

Positive

Alkaloids

Turbidity

No turbidity

Negative

Glycosides

Violet to Blue to

Black Color

Negative

Black Color

Negative

Brown Ring in the

Black Color, No

Negative

Interphase

brown ring

becoming Green

observed

Green Color
Salkowskis

Reddish Brown
Color

Keller- Kiliani

SHORT OVERVIEW PG.14

Phytochemical Analysis Result

caring humanity
SHORT OVERVIEW PG.15

Phytochemical Analysis Result

caring humanity
SHORT OVERVIEW PG.16

Phytochemical Analysis Result

caring humanity

Table 1. Comparison of Treatment Groups at Different Time Intervals Using One- way
ANOVA and Post Hoc (Least Significant Difference)
Post Hoc (Least Significant Difference)

One Way ANOVA


Time Frame
(hours)

P. edulis vs
F-ratio

p-value

Negative
control

P. edulis vs

P. edulis vs

P. edulis vs

Aspirin

Naproxen

Indomethacin

Baseline

12.922

0.000

0.000

0.608

0.267

0.167

20.986

0.000

0.000

0.099

0.731

0.229

12

12.181

0.000

0.000

0.402

0.096

0.017

16

22.602

0.000

0.000

0.383

0.112

0.041

20

11.128

0.000

0.000

0.331

0.052

0.082

24

11.374

0.000

0.000

0.742

0.519

0.221

SHORT OVERVIEW PG.17

Phytochemical Analysis Result

caring humanity

Figure 3. Mean Frequency Grade of Different Treatment Groups on


Histological Degree of Inflammation
SHORT OVERVIEW PG.18

Conclusions and Recommendations

caring humanity

Based from all the gathered results, the


researchers concluded that P. edulis has antiinflammatory property comparable to aspirin
and naproxen from four to 24 hours postinduction of inflammation as well as in
indomethacin except during the 12th and 16th
hour where P. edulis showed better antiinflammatory effect. This conclusion was
supported by the histopathological findings in
the degree of inflammation from the skin nip
biopsy.
SHORT OVERVIEW PG.19

Conclusions and Recommendations


The researchers would like to recommend:

caring humanity

1) consider addition of the number of treatment


administration with respect to the half-life and
duration of action of the different drugs
2) obtain the active constituent of the extract to
maximize the study of its anti- inflammatory
property
3) compare it to other commonly prescribed
2 inhibitor and corticosteroids.

SHORT OVERVIEW PG.20

cox-

References

caring humanity
SHORT OVERVIEW PG.21

caring humanity

Thank You!
If we knew what we were doing it wouldn't be research.
- Albert Einstein

SHORT OVERVIEW PG.22