Green Fluorescent Protein drawn in cartoon style with fluorophore highlighted as ball-andstick; one wholly-reproduced protein, and cutaway version to show the fluorophore.
Review
Central Dogma
5 ATG GAC CAG TCG GTT TAA GCT 3
DNA
3 TAC CTG GTC AGC CAA ATT CGT 5
transcription
RNA
Protein
aa - aa - aa - aa - aa - aa - aa
Protein Structure
via condensation
Protein Structure
Primary Structure
General Structure
H2N
COOH
Nonpolar
ring
(Hydrophobic)
sulfur
Protein Structure
Primary Structure
Protein Structure
Two major types
of Secondary
Structure
Helix
Sheet
Protein Structure
DNA
RNA
Protein
aa - aa - aa - aa - aa - aa - aa
UGA
UAA
UAG
Stop
UAA
UGA
UAG
Stop
UAA
UGA
UAG
Review - RNA
mRNA- messenger RNA
tRNA- transfer RNA
rRNA- Ribosomal RNA
tRNA-The adapter
tRNA-The adapter
-tRNA functions as the adapter
between amino acids and the
RNA template
-tRNAs are structurally similar
except in two regions
Amino acid attachment site
Anticodon
tRNA-The anticodon
The tRNA anticodon
3 base sequence
Complementary to the codon
Base pairing between the mRNA and the tRNA
Oriented and written in the 3 to 5 direction
tRNA
Aspartic Acid
3 CUG 5
5 GAC 3
mRNA
Aminoacyl-tRNA
synthetase
The enzyme responsible
for joining an amino
acid to its
corresponding tRNA
20 tRNA synthetases
1 for each amino acid
Wobble
Allows one tRNA to recognize multiple codons
Occurs in the 3rd nucleotide
of a codon
Wobble Problem
What anticodon would you predict for a tRNA species carrying
isoleucine?
Translation
Synthesizing Protein
An overview
Shine-Dalgarno Sequence
Figure2121
Figure 9-15-1
Figure2121
Figure 9-15-2
Figure2121
Figure 9-15-3
Figure2121
Figure 9-16-1
Figure2121
Figure 9-16-2
4.Eukaryotic Initiation
Factors (eIF4A,
eIF4B, and eIF4G)
associate with the 5
cap, the 40S subunit,
and initiator tRNA
Figure 9-16-3
5. Complex moves 5 to
3 unwinding the
mRNA until an
initiation site (AUG)
is discovered
Figure 9-16-4
6. Initiation factors
are released and 60S
subunit binds
Elongation
Requires two protein Elongation Factors:
EF-Tu and EF-G
Amino acids are added to the growing peptide chain
at the rate of 2-15 amino acids per second
Elongation
Termination
Release Factors RF1, RF2 and RF3
RF1 recognizes UAA or UAG
RF2 recognizes UAA or UGA
RF3 assists both RF1 and RF2
Stop codon also called a nonsense codon
Eukaryotes
NO nuclear membrane
Translation coupled to
transcription
Formylmethionine bound to
initiator tRNA
Posttranslational Folding
Proteins must fold correctly to be functional
Correct folding is not always energetically favorable in the
cytoplasm
Chaperones (including GroE chaperonins) bind to nascent
peptides and facilitate correct folding
Posttranslational modifications
Phosphorylation
Many proteins require some
type of modification to become
functional
Posttranslational modifications
Glycosylation adding sugars
Signaling molecules
Cell wall proteins
Glycoproteins
Posttranslational modifications
Summary
Translation
Prokaryote
Eukaryote