General Outline
Background
Methods
Results
Conclusion
Acknowledgements
Background
General Outline
Methods
Results
Conclusion
Acknowledgements
General Outline
Background
Cardiovascular Disease and Hypertension
Voltage-gated calcium channels (CaL)
Methods
Results
Conclusion
Acknowledgements
Questions
Does OVX alone decrease vascular reactivity
(increased tonicity) in resistance arteries?
Studies using OVX induce pathology
(hypertension)
Other Questions
Part of large project with many others
If OVX mesentery demonstrates increased
voltage-gated Ca2+ channel expression (Rosss
project)
If OVX mesentery demonstrates increased Ca2+
influx (Charmains project)
Significance
41% of postmenopausal have hypertension
75% of women over 60 are hypertensive
Purpose
To study E2s effects on mouse morphology
and physiology
To determine E2s effects on vascular
reactivity in resistance arteries
General Outline
Background
Methods
Mouse dissection
Western blots
Results
Conclusion
Acknowledgements
Methods
Heart Dissection
Hearts dissected for
weight measurement
Whole Heart
RV and LV combined
Right Ventricle
Left Ventricle
Aorta dissection
Mesentery Dissection
Mesentery Dissection
Mesentery Dissection
Mesentery Dissection
3
1
3
4
1
Methods-Vascular Reactivity
Mesenteric arteries
Second order
150-200 m lumen diameter
Background
Methods
Results
General Outline
Morphological Changes
Vascular Reactivity
Conclusion
Acknowledgements
Vascular Reactivity
n=2
n=3
n=4
n=3
n=2
n=1
Vascular Reactivity
n=2
n=3
n=4
n=3
n=2
n=1
General Outline
Background
Methods
Results
Conclusion
Acknowledgements
Conclusions
Age effect on body mass, but no
treatment effect
Treatment effect on uterine mass
Trends, but no significant effect, on
heart masses
Conclusions
No significant effect on vascular
reactivity
Limited sample size
Conduit v. resistance arteries
Generation of spontaneous tone at 80
mm Hg
Tone increased 25% from 60 mm Hg
General Outline
Background
Methods
Results
Conclusion
Acknowledgements
Acknowledgements
My committee members:
Brent J.F. Ph.D.
Jeff Padberg Ph.D.
Ben Rowley Ph.D.
Other Faculty:
Kristie Hicks Ph.D.
Mike Martin M.D.
Lab Members:
Renee Jordan
Ross Gray
Charmain Fernando
Edouard Niyonsaba
Mohamed Idrissa Moussa