histology of Tooth

Development

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Stages of Tooth Development

Dental lamina
Bud
Cap
Bell
Appositional
Repeated by Successional lamina
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 Early Dental Lamina

Tongue Dental lamina Vestibular lamina

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 Bud
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 Cap Stage of
Tooth
Development
Dental (enamel) organ

Dental papilla

Dental follicle

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Copyright 2007, Thomas G. Hollinger, Gainesville, Fl
 Cap Stage

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Copyright 2007, Thomas G. Hollinger, Gainesville, Fl
 Bell Stage

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Oral Histology, 5th edition, A R Ten Cate
Copyright 2007, Thomas G. Hollinger, Gainesville, Fl
Oral cavity

Outer dental
epithelium

Dental lamina

Enamel knob

Stellate reticulum

Stratum intermedium

Inner dental
Epithelium

Dental papilla
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Structures Seen at Bell Stage

Cervical Loop

Acellular zone

Preameloblasts
(IDE or IEE)

Stratum intermedium

Stellate reticulum

ODE (OEE)

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 Cervical loop Dental follicle

Acellular zone
Dental organ

Structures of ODE
the Dental Stellate
Organ reticulum

Stratum
Dental papilla intermedium

IDE
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 Appositional
Stage

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Oral Histology, 5th edition, A R Ten Cate
 Dental follicle

ODE

Stellate reticulum

IDE

Pulp

Odontoblasts

Stratum intermedium

Structures at Appositional Stage

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 Dentinogenesis

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Oral Histology, 5th edition, A R Ten Cate
 Details of
Tooth
Development
Cell free zone

Odontoblasts

Ameloblasts

Preameloblasts

Preodontoblasts
Ameloblast
Acellular zone with
Tomes process
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Oral Histology, 5th edition, A R Ten Cate
Incorporation of
Tetracycline
during
Dentinogenesis

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 Some Types of
Dentin
Mantle dentin

Tertiary dentin

Primary dentin

Secondary dentin

Predentin

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Oral Histology, 5th edition, A R Ten Cate
 Amelogenesis

Enamel proteins
Amelogenins (Bulk of proteins)
Tuftelin (Just at the DEJ)
Enamelin 2%
Amelins/Ameloblastins 5-10%

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Early Apposition

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Distal End of a Ameloblast

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Distal End of a Ameloblast
Forms rod sheath

Forms enamel rod

Forms
interrod
substance Dist
al proc
ess

Proximal portion of process

Junctional
complex

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Tomes
Process

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 Enamel Secretory
Ameloblast

Tomes process

Junctional complex

Cell body of ameloblast

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Oral Histology, 5th edition, A R Ten Cate
 Grazing Section
through Forming
Enamel
Interrod substance
forms first to establish a
mold for the enamel rod

Terminal bar
(in TEM one sees the
junctional complex)
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Developing
Enamel

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Root Formation Cervical loop forms the epithelial
root sheath (of Hertwig)

Cervical loop

Dental papilla

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Root Formation

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Cells of the dental follicle (forms cementocytes)
Cell rest (of Malassez)

PDL, Cell Rests, and Cementum

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Granular Layer of Tomes

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Cellular Cementum
Acellular cementum

Cellular cementum

Hyaline layer
(of Hopewell Smith)

Granular layer of Tomes

Dentin (with tubules)

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 Junctional
Epithelium

Basement membrane
shown in green

B = Alveolar mucosa

C = Sulcular epithelium

D = Basement membrane of
junctional epithelium
(derived from the reduced
enamel epithelium)

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Thanx. !!

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