pathogenicity and
epidemiology
Introduction
Some organisms may cause disease through ingestion
of substances (toxins) produced during microbial growth
on foods
Clostridium botulinum botulism
Bacillus cereus vomiting
Resident microbiota
Transient microbiota
Microbiome
Mutualism both benefit
Commensalism one benefits without causing harm to
the other
Skin
Respiratory tract
Intestinal tract
Urogenital tract
conjuctiva
Portal of entry (SKIN)
The major part of the body that is widely exposed to
microorganisms
Infection:
Use of contact lens
Damage of conjunctiva
Exposure to chemicals
Reduction of tear flow
Consolidation
A pathogen must be able to survive at its initial portal of entry
Evade normal flora
Evade macrophages & WBCs
Attach to a surface (biofilm)
Adhesive substances mucopeptides, mucopolysaccharide layer
Pili
agglutinins
Avoidance of opsonization
process of identifying the organism to the phagocyte
Protein-A (S. aureus)
Survival following phagocytosis
Microorganism can survive following phagocytosis when
it avoids the killing and digestion process within the
phagocyte
Kill phagocyte - production of leucocidins - (lysosomal
substances)
Non-invasive Pathogens
Production of toxins
Partially invasive Pathogens
Attach to mucosal epithelia and penetrate rapidly into the epithelial cells
Prevention
Spread of infectious disease in a population of individuals
Vaccination programs
BIOFILMS
Introduction
Bacteria that exist as adherent microcolonies (biotic/abiotic
surfaces)
Nosocomial infections
Tolerance of biofilms to
antimicrobials
Advent of antibiotics
Antibiotic resistant strains of bacteria
Lack of responsiveness to antimicrobial therapy
Recurrent UTIs
For antibiotic to be effective requires 1000x more
concentration which is not safe to patients
Adaptation of biofilm - Altered tolerance to drugs
Only in biofilm mode and not in planktonic mode
Mechanisms of biofilm tolerance
- Compact and highly charged matrix surrounding the
biofilm
(prevents penetration of antibiotics)
- Charge of antibiotic affects penetration
Not highly charged antibiotic easily penetrate the matrix
Highly charged antibiotic delayed penetration