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Referat Ilmu Kedokteran Forensik dan Medikolegal

DNA SIDIK JARI

Oleh :
ANDI NURUL PRATIWI
ANDI DEWI SANRA SANRIKA
KHAIRUNNISA

Pembimbing :
dr. STEPHANIE RANNI ANINDITA

Supervisor :
dr. ULFA CAMELIA INDIASARI, M.Kes, Sp.F
SEJARAH DNA SIDIK JARI

Kasus pertama yang menggunakan


hasil analisis DNA sebagai bukti

Pemerkosaan dan pembunuhan dua


orang gadis berusia 15 tahun
- Lynda Mann dan Dawn Asworth -
pada tahun 1984 di Inggris
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
Buckleton John, Triggs Christopher, Walsh Simon. Forensic DNA Evidence Interpretation. 2005. USA : CRC Press.
Yount Lisa. Milestones in Discovery and Invention Forensic Science : from Fibers to Fingerprints. 2007. USA : Chelsea House.
Profesor Sir Alec Jeffreys

Vaginal swab Vaginal swab


Lynda Mann - 1984 Dawn Asworth - 1986

1 orang pelaku yang sama


Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
Buckleton John, Triggs Christopher, Walsh Simon. Forensic DNA Evidence Interpretation. 2005. USA : CRC Press.
Yount Lisa. Milestones in Discovery and Invention Forensic Science : from Fibers to Fingerprints. 2007. USA : Chelsea House.
Pengumpulan sampel darah semua
laki-laki golongan usia tertentu di
sekitar Tempat Kejadian Perkara

ANALISIS DNA

Darah Vaginal Swab

Colin Pitchfork
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
Buckleton John, Triggs Christopher, Walsh Simon. Forensic DNA Evidence Interpretation. 2005. USA : CRC Press.
Yount Lisa. Milestones in Discovery and Invention Forensic Science : from Fibers to Fingerprints. 2007. USA : Chelsea House.
DNA SIDIK JARI
Uji paternitas
Identifikasi
Deteksi perubahan genetis tumor
Diagnosis antenatal penyakit herediter
Analisis marker sebelum transplantasi
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
Kirby Lorne. DNA Fingerprinting : An Introduction. 1992. New York : Oxford University Press.
STRUKTUR DNA

Wahyu, Warsidi, Reissa. Apakah DNA. 2010. Bandung : Puri Delco.


Basa Purin
Adenin
Guanin

Basa Pirimidin
Sitosin
Timin

Ikatan Hidrogen

Fosfat

Deoksiribosa

Yount Lisa. Milestones in Discovery and Invention Forensic Science : from Fibers to Fingerprints. 2007. USA : Chelsea House.
Michaelis Ron, Flanders Robert, Wulff. A Litigators Guide to DNA : from The Laboratory to The Courtroom. 2008. USA : Elsevier.
Dalam uji DNA forensik, analisis
difokuskan pada basepairs (bp)

Polimorfisme DNA

Michaelis Ron, Flanders Robert, Wulff. A Litigators Guide to DNA : from The Laboratory to The Courtroom. 2008. USA : Elsevier.
National Forensic Science Technology Center. A Simplified Guide to DNA Evidence. 2009. USA : Bureau of Justice Assistance.
DNA Nuklear dan DNA Mitokondria

DNA Nuklear DNA Mitokondria

Guyton Arthur, Hall John. Textbook of Medical Physiology 11th Edition. 2006. China : Elsevier.
SAMPEL DNA
KORBAN HIDUP
Darah
Cairan semen
Rambut dengan akarnya
Sel epitel (mukosa buccal, saliva, touch DNA)

KORBAN MATI
Jaringan lunak (otot)
Jaringan keras (gigi dan tulang)
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
International Committee of The Red Cross. Missing People, DNA Analysis and Identification of Human Remains : A Guide to
Best Practice in Armed Violence 2nd Edition. 2009. Switzerland.
Kandungan DNA Sampel

Kirby Lorne. DNA Fingerprinting : An Introduction. 1992. New York : Oxford University Press.
Lee Henry, Ladd Carll. Preservation and Collection of Biological Evidence. Croatian Medical Journal 42(3) : 225-228. 2001. United
States of America : Forensic Science Laboratory of Connecticut Department of Public Safety.
Catalin Marian, Andrei Anghel, Mitrasca Oana. Modern Methods of Collection and Preservation of Biological Evidence for Human
Identification by DNA Analysis. 2000. Timisoara : Biochemistry Department of Victor Babes
D
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National Forensic Science Technology Center. A Simplified Guide to DNA Evidence. 2009. United States of America : Bureau of Justice Assistance.
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National Forensic Science Technology Center. A Simplified Guide to DNA Evidence. 2009. United States of America : Bureau of Justice Assistance.
National Forensic Science Technology Center. A Simplified Guide to DNA Evidence. 2009. United States of America : Bureau of Justice Assistance.
Pengambilan Sampel

Sel darah putih Spermatozoa Akar rambut Epitel kulit

Analisis DNA
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
REFERENCE SAMPLE

Sampel dari suspect atau victim

Buccal swab atau darah vena dari :


- Orang tua kandung
- Saudara kandung
- Anak
- Saudara se-Ibu
- Saudara se-Ayah

Artefak :
- Sikat gigi
- Sisir
- Alat cukur
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
International Committee of The Red Cross. Missing People, DNA Analysis and Identification of Human Remains : A Guide to
Best Practice in Armed Violence 2nd Edition. 2009. Switzerland.
PROSES PEMETAAN DNA

Restriction Fragment
Polymerase Chain
Length Polymorphism
Reaction (PCR)
(RFLP)

Variable Number Short Tandem


Tandem Repeats
(VNTRs) Repeats (STRs)

Kirby Lorne. DNA Fingerprinting : An Introduction. 1992. New York : Oxford University Press.
Wahyu, Warsidi, Reissa. Apakah DNA. 2010. Bandung : Puri Delco.
Petricevic Susan, Heather Wansbrough. DNA Profiling in Forensic Science in XII Biotechnology and DNA Profiling. 2000.
DNA PROFILING

Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
International Committee of The Red Cross. Missing People, DNA Analysis and Identification of Human Remains : A Guide to
Best Practice in Armed Violence 2nd Edition. 2009. Switzerland.
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
International Committee of The Red Cross. Missing People, DNA Analysis and Identification of Human Remains : A Guide to
Best Practice in Armed Violence 2nd Edition. 2009. Switzerland.
Goodwin William, Linacre Adrian, Hadi Sibte. An Introduction to Forensic Genetics. 2007. England : John Wiley & Sons Ltd.
International Committee of The Red Cross. Missing People, DNA Analysis and Identification of Human Remains : A Guide to
Best Practice in Armed Violence 2nd Edition. 2009. Switzerland.c
- TERIMA KASIH -