Template of Poster for International Seminar

AISTEEL, October 16-17, 2017

Saronom Silaban1,2 , D. Sofian Wildan1, Siti Nur Inayah1, Sutarya Enus3, Iman P.
Maksum1, Khomaini Hasan4, Toto Subroto1*, and Soetijoso Soemitro1
1Postgraduate Program, Universitas Negeri Medan, Medan, Indonesia,2Department of Chemistry Education, Universitas
Negeri Jakarta, Jakarta, Indonesia, 3...etc.
*Corresponding authors: saronom@unimed.ac.id

Abstract 2. Solubilisation of rhPT2 in different strategies

Table 1. rhPT2 expression at different chaperone modulation.

This template was designed to produce a 594 mm x 841mm (A1
size) poster in portrait or landscape orientation. Weve put in the
headings we usually see in these posters, you can copy and paste E. coli Chaperone rhPT2 (thiol) rhPT2 (pH)
and change to your hearts content! We suggest you use keep black modulation
S I S I
text against a light background so that it is easy to read.
Background color can be changed in format-background-drop -
ER2566
down menu.
DnaK, DnaJ, GrpE,
ER2566 GroEL and GroES

Background ER2566 DnaK, DnaJ, and

GrpE
Size of poster is
Because of important function in clot formation thrombin has been ER2566 GroEL and GroES

utilised in a number of product intended for haemostasis and/or as

tissue sealants. The recombinant human prethrombin-2 (rhPT2), Arctic Cpn10 and Cpn60
as a thrombin precursor, is a protease-like protein which is Express
expressed by E. S : soluble; I: insoluble

3. Mechanism of chaperone
Method A B

pG-KJE8
Figure 2. Chaperone cycle of (A) GroEL and GroES, and (B) DnaK, DnaJ, and
GrpE.

ArcticExpress and ER2566-chaperone co-expression successfully

expressed soluble rhPT2. GroEL/GroES and Cpn60/Cpn10 are the
best chaperone systems for expressing soluble rhPT2.

ArcticExpress ER2566 ER2566

Expression
SDS-PAGE
Conclusions
Results
The solubilisation of the rhPT2 fusion intein-tag was achieved by
chaperone modulation. These results revealed that chaperones are
Results tools for protein folding and assists the solubilisation process of
rhPT2 in E. coli.
1. Cloning of rhPT2 into pTWIN1
A B
Acknowledgments
6000 M 1 2 pTWIN1 6000
M 1 pTWIN1
2
This work is supported by grant Penelitian Unggulan Strategis
Nasional (PUSNAS) from Directorate of Higher Education,
Ministry of National Education, Indonesia.
1000 1000
rhPT2 (thiol) rhPT2 (pH)

References
Figure 1. Cloning of (A) rhPT2 (thiol) and (B) rhPT2 (pH) into pTWIN1. M Soejima et al. 2001. J Biochem. 130, 269-277
is marker, 1. undigested plasmid and 2. double- Strocchi et al. 2006. Proteomics. 6, 193-206
digested plasmid resulting pTWIN1 and rhPT2. Walter and Buchner. 2002. Angew. Chem. Int. Ed. 41, 1098-1113