STUDY POPULATION
The samples studied were excess, cell-free BALF collected
longitudinally between 2002-2009 from routine post-
transplant surveillance bronchoscopies as previously
described.
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Proteins were separated and concentrated
offline in the 1st dimension into 15 peptide-
containing fractions, collected in 2-minute
intervals on a C18 Gemini column.
Short time-series expression miner
(STEM)
We investigated how the protein profiles changed
longitudinally as the patient progressed towards
BOS using STEM analysis.
The STEM algorithm and software identifies genes
that have similar behavior over a short time series
(3-8 time-points) with all genes clustered into one
of a set of pre-defined patterns based on
transformation of gene profiles into units of
change.
Result
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