The normal urine sediment may contain a variety of formed elements.
Cellular elements are easily distorted by
the widely varying concentrations, pH, and presence of metabolites in urine, making identification more difficult.
URINARY SEDIMENT CONSTTITUENTS
RED BLOOD CELLS Smooth, non-nucleated, biconcave, disks measuring approximately 7 mm in diameter RED BLOOD CELLS Routinely reported as the average number seen in 10 hpfs. Concentrated (hypersthenuric) urine - the cells shrink due to loss of water and may appear crenated. Dilute (hyposthenuric) urine - the cells absorb water, swell, and lyse rapidly, releasing their hemoglobin and leaving only the cell membrane. Microcytic and crenated RBCs (× 100) RED BLOOD CELLS RBCs are frequently confused with yeast cells, oil droplets, and air bubbles. The rough appearance of crenated RBCs may resemble the granules seen in WBCs. Remember: Yeast cells usually exhibit budding.
Oil droplets and air bubbles are highly refractile
when the fine adjustment is focused up and down.
RBCs are much smaller than WBCs. Oil droplet (400x)
Adding acetic acid to
a portion of the sediment will lyse the RBCs, leaving the yeast, oil droplets, and Air bubble (100x) WBCs intact. RED BLOOD CELLS RBCs that vary in size, have cellular protrusions, or are fragmented are termed dysmorphic and have been associated primarily with glomerular bleeding. Abnormal urine concentration affects RBC appearance. Small number of dysmorphic cells are found with nonglomerular hematuria. Dysmorphic RBCs (×400) CLINICAL SIGNIFICANCE
The presence of RBCs in the urine is associated
with damage to the glomerular membrane or vascular injury within the genitourinary tract. Macroscopic analysis - cloudy with a red to brown color Microscopic analysis - may be reported in terms of greater than 100 per hpf or as specified by laboratory protocol. CLINICAL SIGNIFICANCE The observation of microscopic hematuria can be critical to the early diagnosis of glomerular disorders and malignancy of the urinary tract and to confirm the presence of renal calculi.
The presence of not only RBCs but also hyaline,
granular, and RBC casts may be seen following strenuous exercise. CLINICAL SIGNIFICANCE The presence of hemoglobin that has been filtered by the glomerulus produces a red urine with a positive chemical test result for blood in the absence of microscopic hematuria.
Specimen appearing macroscopically
normal may contain a small but pathologically significant number of RBCs when examined microscopically. TAKE NOTE ! WHITE BLOOD CELLS WBCs are larger than RBCs, measuring an average of about 12 mm in diameter. WHITE BLOOD CELLS Neutrophils contain granules and multilobed nuclei. They lyse rapidly in dilute alkaline urine and begin to lose nuclear detail. Neutrophils exposed to hypotonic urine absorb water and swell. Glitter cells sparkling appearance, produce from the Brownian movement of the granules within these larger cells. Sternheimer-Malbin stain - large cells stain light blue as opposed to the violet color usually seen with neutrophils. Glitter cells WHITE BLOOD CELLS Eosinophils - primarily associated with drug- induced interstitial nephritis. Small numbers of eosinophils may be seen with urinary tract infection (UTI) and renal transplant rejection. Urine sediment may be concentrated by routine centrifugation alone or with cytocentrifugation. Hansel preferred eosinophil stain.
Eosinophils are not normally seen in the urine.
Hansel-stained eosinophils (×400) WHITE BLOOD CELLS Mononuclear cells Lymphocytes are the smallest WBCs, they may resemble RBCs. They may be seen in increased numbers in the early stages of renal transplant rejection. Monocytes, macrophages, and histiocytes are large cells and may appear vacuolated or contain inclusions inclusions. WHITE BLOOD CELLS An increase in urinary WBCs is called pyuria and indicates the presence of an infection or inflammation in the genitourinary system. Pyelonephritis, cystitis, prostatitis, and urethritis, are frequent causes of pyuria – bacterial disorders Glomerulonephritis, lupus erythematosus, interstitial nephritis, and tumors - nonbacterial disorders WBCs with acetic acid nuclear enhancement. TAKE NOTE ! BACTERIA BACTERIA Bacteria may be present in the form of cocci (spherical) or bacilli (rods). They are reported as few, moderate, or many per high-power field. To be considered significant for UTI, bacteria should be accompanied by WBCs. Observing bacteria for motility also is useful in differentiating them from similarly appearing amorphous phosphates and urates. BACTERIA The presence of bacteria can be indicative of either lower or upper UTI. Specimens containing increased bacteria and leukocytes are routinely followed up with a specimen for quantitative urine culture. The bacteria most frequently associated with UTI are the Enterobacteriaceae (referred to as gram negative rods) BACTERIA The cocci-shaped Staphylococcus and Enterococcus are also capable of causing UTI.
The actual bacteria producing an UTI cannot be
identified with the microscopic examination. A. Rod-shaped bacteria often seen in urinary tract infections
B. KOVA-stained bacteria and WBC (×400) TAKE NOTE ! Source: Urinalysis and Body Fluids SIXTH EDITION Susan King Strasinger, DA, MLS(ASCP)