PROBLEM 6.

8 BIOCHEMICAL
ENGINEERING
BY JAMES M LEE
CELL KYNETICS and FERMENTER DESIGN
Suppose organism that obeys Monod Equation:

Is being cultivated in steady state CSTF with the

following data:

F = 100 L/hr
µmax = 0.5 hr-1
Ks = 2 g/L
CSi = 50 g/L
YX/S = 0.5
a) What size vessel will give the maximum total
rate of shell production
b) What are the substrate and cell
concentrations of the optimum fermenter in
part a)
c) If existing flow from the fermenter in part a) is
fed to a second fermenter (CSTF), what
should be the size of the second fermenter to
reduce the substrate concentration to 1 g/L
d) If the existing flow from the first fermenter in
part a) is fed to second fermenter which size
is the same as the first, what will be the cell
and substrate concentrations leaving the
second fermenter
 ......................Equation 6.40

2 𝑔/𝐿 + 50 𝑔/𝐿
𝛼=
2 𝑔/𝐿

𝛼 = 26 = 5,1

........................Equation 6.42

5.1
𝜏𝑚, 𝑜𝑝𝑡 =
0.5 (5.1 − 1)
𝜏𝑚, 𝑜𝑝𝑡 = 2.48 jam
a) Ukuran vessel
𝑉
𝜏𝑚 =
𝐹
V = 𝜏𝑚 . F
= 2.48 jam x 100 L/jam = 248 L

b) Konsentrasi keluaran substrat dan cell

.......eq 6.39 .....eq. 6.32
5.1
Cx, opt = 0.5 x 50 g/L 20.9 = 0.5 (50 – Cs)
5.1+1
Cx, opt = 20.9 g/L 10.45 = 50 – Cs
Cs = 39.55 g/L
c) Hitung ukuran fermenter (V) jika keluaran fermenter 1
masuk ke fermenter 2 untuk menjadikan konsentrasi
substrat menjadi 1 g/L
Sehingga pada fermenter 2:
CSi = 1 g/L
CX = 20.9 g/L
CS = 39,55 g/L
YX/S = 0.5
𝐶𝑋𝑖 − 𝐶𝑋
𝑌𝑋/𝑆 = … eqs 6.51
𝐶𝑆 −𝐶𝑆𝑖
𝐶𝑋𝑖 −20.9
0.5 =
39.55 −1

CXi = 40.175 g/l

 F(CXn-1- CXn) + Vn rxn = 0 ………..eqs(6.50)

𝜇𝑚𝑎𝑥 𝐶𝑋 𝐶𝑆𝑖
 F(CX- CXi) + V2 =0
𝑘𝑆 +𝐶𝑆𝑖

𝐹(𝐶𝑋𝑖 −𝐶𝑋 )(kS +𝐶𝑆𝑖 )

 V2 =
𝜇𝑚𝑎𝑥 𝐶𝑋𝑖 𝐶𝑆𝑖

100(40.175−20,9)(2 +1)
 =
(0.5)(1)(40.175)

 = 287,8656 L
d) Tentukan konsentrasi subtrat dan sel yang keluar
dari fermeter kedua jika volume fermenter 2 sama
dengan volume fermenter 1

𝐶𝑋𝑖 − 𝐶𝑋
𝑌𝑋/𝑆 = …...………… eqs 6.51
𝐶𝑆 −𝐶𝑆𝑖

𝐶𝑋𝑖 −20.9
0.5 =
39.55−𝐶𝑆𝑖

CXi = 19.775 – 0.5 CSi + 20.9

= 40.675 – 0.5 CSi …………………….(1)

 F( CXn-1- CXn) + Vn rxn = 0 ………..eqs(6.50)

𝜇𝑚𝑎𝑥 𝐶𝑋 𝐶𝑆𝑖
 F( CX- CXi) + V2 =0
𝑘𝑆 +𝐶𝑆𝑖

𝐹(𝐶𝑋𝑖 −CX )(kS +𝐶𝑆𝑖 )

 V2 =
𝜇𝑚𝑎𝑥 𝐶𝑋 𝐶𝑆𝑖

𝐹(𝐶𝑋𝑖 −CX )(kS +𝐶𝑆𝑖 )

 248 =
𝜇𝑚𝑎𝑥 𝐶𝑋 𝐶𝑆

100(𝐶𝑋𝑖 −20,9)(2 +𝐶𝑆𝑖 )

 =
0.5 20.9 𝐶𝑆𝑖

 2CXi – 0.24CXiCSi - 20.9CSi – 41.8 = 0 ………... (2)

 Substitusi pers (1) ke (2) sehingga didapatkan

CSi = 1.25 g/L

 Subtitusi nilai CSi ke pers (1)

CXi = 40.675 – 0.5 CSi

= 40.675 – 0.5 (1.25510)

= 40.05 g/L