Indah lestari
1503113400
C-13
• Currently turmeric farmers have problems in selling the crops because of the
unavailability appropriate technology for farmers to be able to perform curcumin
content independently.
• Therefore, from this research will be made measuring instrument ef kurkuminoid
level based on visible spectrophotometric method for harvest sorting needs based
on market qualification for turmeric rhizome farmer with cheap and easy
component used by farmer, considering testing of curcumin level in tumeric
rhizome with instrumentation on stndard laboratory costs are high enough.
• In this study will be measured levels of curcuminoid contained in the rhizome of
fresh turmeric ( curcuma domestica ) grown in some areas of turmeric rhizome
producer in the area of central java and yogyakarta with different planting altitudes.
Literrature review
Take 5 ml curcumin in soltion 1. insert into ml flask. Add ethanol to the mark ( solution 2)
v
Sample of turmeric crushed using mortar add technical ethanol to 200 ml
measure absorbance
By obtaining the y value of this measurement and the value of variables a and b are known, the curcumin value of
solution(x) will be abtained according to the above standard curve equation by the formula :
𝑦−𝑎
X= 𝑏
Results and discussion
Absorbance
(y)
Curcumin Voltage diffraction
solution (ppm) Voltage monochromator grating
prism(mV) monochromator Spectrophotometer
(Volt)
1 (Karanganyar) 5,2 0,92 0,156
2 (Magelang) 5,6 2,24 0,306
3 (Imogiri) 5,7 3,28 0,461
4 (Wonosobo) 6,5 3,4 0,612
5 (Wonogiri) 6,7 4,35 0,764
Table 1. standard curve measurement results Picture 1. calculation of raw curve equation
and linearity level
From FIG. 1 it appears that the results obtained from the measurements using standard
spectrophotometers produced linear graphs with linearity level 1, whereas for 0,941 diffraction grating
and for prism of 0,943.
It appears that with the monochromator results with the monochromator are slighty more linear than
those of diffraction grating monochromator. This linearty level can still be improved by making the
cover along the beam of light,
So the measurement is not affected by the light from outside the light source. The received voltage
from the transmitted lightof the prism is very small, so it needs to be given a amplifier circuit.
Absorbance (y)
Turmeric solution from the Voltage diffraction grating
NO region Voltage monochromator prism (mV) monochromator(Volt) Standard spectrophotometer
Table 2 shows the absorbance of turmeric samples from various planting areas using three
measuring instruments. Of these results are then used to measure the level of curcumin in
turmeric.
Turmeric Kadar (x) Error (%)
Standard
solution from Prism spectrophotomet Prism diffraction
the region diffraction grating er
NO grating
• This difference results because the measurement is still in open condition, so there is a possibility of
being affected by the outside light
• In addition, the sample is measured after two hours of samples are extracted and located in the
environment
• Therefore the prototype of this curcumin level gauge still needs to be repaired, both appliance repair
and measurement method.
Conclusion
Kurniasih, G., Asmiyanti Djaliasrin Djalil, Dwi Hartanti. (2007). Penetapan Kadar
Kurkuminoid dalam Jamu Serbuk Galian Putri yang Mengandung Simplisia
Rimpang Kunyit (Curcuma Domestica Val.) yang Beredar di Kecamatan
Ketanggungan.
http://jurnal.ump.ac.id/index.php/pharmacy/article/download/217/209.