GCF

(Gingival Crevicular Fluid)

content
• Introduction
• Transudate/Exudate
• Component
• Function
• GCF Defense Mechanism
INTRODUCTION
 GCF is a complex mixture of
subtance derived from serum,
leukocytes, structural cell of
periodontium and oral bacteria
 These subtances possess a great
potential for serving as indicators of
periodontal disease and healing
after therapy
Transudate/
Exudate
Transudate/Exudate
Component
Enzymatic
• Host derived & other
product
• Bacteria derived

Non-Enzymatic
• Cellular
• Electrolytes
• Organic Compound
Enzymatic Component
Host derived Bacteria derived
 Acid phosphatase  Hyaluronidase
 Alkaline phosphate  Acid phosphatase
 B-glucouronidase  Alkaline phosphate
 Proteolytic Enzyme  Collagenase
 Elastase  Phospholipase-A
 Collagenase  Phospholipase-C
Alkaline Phosphate
From PMN
Role : calcification
B-glucouronidase
Lysozomal enzyme
Source : macrophages,
fibroblast, endothelial cells,
bacteria
Role : catabolism of
mucopolysaccharides,
degradating oligosaccharides
Collagenase
Derived from PMN,
macrophages, fibroblast,
keratinocytes, osteoclast,
bacteria
GCF from chronic site of
periodontitis exhibit elevated
collagenolytic activities
compared to GCF from healty
site
Elastase
 Abundant proteinase from
neutrophils
 Indicator of neutrophil activity
 Increased elastase in GCF =
periodontal attachment lose
 Can degrade host intercellular
matrix component (elastin,
collagen)
Hyaluronidase
Role : splits link in
bacterial cell wall (which
result in producing
oligosaccharides)
 Non-Enzymatic Component
Organic
Cellular Electrolytes Compound
 Epithelial cell  Sodium  Carbohydrate
 Leukocytes  Potassium  Protein
 Bacteria  Fluoride  Lipid
 Calcium  Metabolic
 PO4 End Product
 Mg
I
Leukocytes
Protein
IgA, IgG, IgM– plasma
cell
Albumin, fibrinogen,
transferrin, bradykinin
Complement
Protease inhibitor
Metabolic End Poduct
 LacticAcid
 Hydroxyproline
 Prostaglandin
 Urea
 Endotoxin
Function
Cleanse material from the sulcus
Contain plasma protein that may
improve adhesion of the epithelium
to the tooth
Possess antimicrobial properties -
lysozyme
One of the gingival defense
mechanisms
GCF Defense
Mechanism
Thank you
Saliva

Cairan kompleks yang

berisi hasil sekresi dari
glandula, baik mayor
maupun minor
content
• Introduction
• Component
• Function
• Defense Mechanism
Function
 Membasahi rongga mulut
 Membantu dalam proses pencernaan
 Mempunyai aktifitas buffering
 Membantu proteksi rongga mulut
Defense Mechanism
 1. Komponen anorganik

 2. Komponen organik
 Antibakteria
 A. salivari aglutinin
a. IgA sekretori
 Adalah imunoglobulin yang paling penting
dalam saliva
 Berfungsi dalam mencegah perlekatan bakteri
pda permukaan mukosa
b. Fikronektin
Lisosim
 Berasal dari glandula saliva mayor
terutama submandibula dan sublingualis,
sel fagosit, sel leukosit, dan cairan limfatik
 Berfungsi sebagai baktriosid
Hidrogen peroksidase
 Berasal dari galndula salivarius dan sel
leukosit
 Melindungi sel dari toksik hydrogen
peroksida
 Bakteriosid dan bakteriostatik
Laktoferin
 Berasal dari glandula salivarius minor
 Sel PMN banyak mengadung Laktoferin
 Bakteriosit terhadap s. mutans
 bakteriostatik
 Mengikat ion besi sehingga menurunkan
level ion besi