Analisis Instruksional

Mata Kuliah Teknologi Fermentasi

Menerapkan Teknik Fermentasi Pada Industri
Pangan dan Hasil Pertanian

Memanen Produk Fermentasi Mengoperasikan Fermenter

Merancang Peralatan
Fermentasi Menumbuhkan mikroba

Menjelaskan Menjelaskan Membuat Media Memelihara Membuat

Bentuk dan Kinetika Fermentasi Biakan Mikroba Inokula
Fungsi Fermentasi
Fermenter

Entry Behavior

Matematika, Fisika, kimia,biokimia,Mikrobiologi

 Desain Bioreactor/
Fermenter

Latar Belakang
• Kontaminasi

• Tidak homogen
• Teknik fermentasi

Controlled Environment
 Beda aseptic dan sterile
 bagaimana menciptakan kondisi
anaerob
 Hal yang perlu diperhatikan

1. Bisa dioperasikan secara aseptik

2. Reliable (long term operation)
3. Memenuhi standart perundang-undangan
4. Tersedia perlengkapan aerasi dan agitasi

5. Konsumsi power rendah

6. Memiliki sistem pengaturan temperature
7. Memiliki perlengkapan pengaturan pH

8. Memiliki sampling port

 9. Evaporasi harus bisa dikontrol

10. Pengoperasian dan maintenance mudah

11. Dapat dirangkai sesuai dengan teknik

fermentasi yang dikehendaki

12. Permukaan vessel smooth, termasuk joints

13. Similar geometry untuk kemudahan scale-up

14. Bahan konstruksi murah

15. Dilengkapi dengan peralatan penunjang

Bioprocessing plants
for the production of
pharmaceuticals

Fermentors for
cultivation of
bacteria (E.coli)
Diagram of a fermenter with one multi-bladed impeller
Diagram of a fermenter with three multi-bladed impellers
ASEPTIC OPERATION AND CONTAINMENT
Non-genetically engineered organisms may be placed into
a hazard group (4) using criteria to assess risk such as:

1. The known pathogenicity of the micro-organism.

2. The virulence or level of pathogenicity of the micro-
organism
3. The number of organisms required to initiate an
infection.
4. The routes of infection.
5. The known incidence of infection in the community and
the existence locally of vectors and potential reserves.
6. The amounts or volumes of organisms used in the
fermentation process.
7. The techniques or processes used.

GILSP (Good Industrial Large Scale Practice)

 BODY CONSTRUCTION

Glass fermenter with a top-flanged carrying plate

(Inceltcch L.H. Reading, England).
Three glass fermenters with top and bottom plates
(New Brunswick Scientific, Hatfield, England
Stainless steel fully autornatic 10 dm' fcrmenter sterilizable-in-situ
Aeration and Agitation

The structural components of the

fermenter involved in aeration and
agitation are:

Impeller (agitator)

Stirrer gland and bearings

Baffles

Sparger (aeration system)

Arrangement for a pair of Intermig agitators.
Relative dimensions are given as a proportion of the fermenter vessel
diameter (T) (Nienow, 1990).
A simple stirrer seal based on a description given by
Rivett et al. (1950)
 Maintanance of aseptic conditions

An arrangement of packed air filter and fermenter (Richards, 1968).

An alternative simple sample port.
Sample system for level 1 (Jannsen et al., 1990).
Air-lift fermenter with outer loop (Taylor and Senior, 1974).
Air-lift fermenter with inner loop (Smith, 1980).