IDENTIFIKASI

Streptococcus sp.
Muhammad Reyhan Pratama

G0016156
 Streptococcus sp.
The name streptococci (streptos meaning twisted or
coiled) was given by Billroth
 STREPTOCOCCUS

• Highly virulent and less virulent (commensal)

• Gram positive bacteria
• Hosts : humans and mammals
• Often found to colonize the mucosal surfaces of the
mouth, nares, pharynx.
• As a pathogen, may also inhabit the skin, heart, or
muscle tissue.
 CHARACTERISTIC

 Gram positive cocci  Optimum temperature:

 Chains or pairs 37o C (35o-43o)
 Usually capsulated  Optimum Ph : 7,4
 Facultative anaerobes
 No flagella  non motile
 Have filli (fimbriae)
 Fastidious
 Catalase negative
 Non spore forming
Anaerob fakultatif
Fastidious organism: has a complex nutritional requirement. In other
words, a fastidious organism will only grow when specific nutrients
are included in its diet. Group A streptococcus (GAS) requires a
minimum concentration of nutrients such as sugars and peptides for best
growth.
Streptococcus -> produced the large amount of hydrogen peroxide; to
growth requires a source of catalase (e.g. blood) to neutralize.

Perbedaan pili dengan flagella:

Flagella -> alat gerak, terdiri dari protein flagelin (bulu cambuk,
jumlahnya sedikit)
Pili -> fungsi adhesi bakteri dg sel tubuh hospes (1) dan konjugasi
antar bakteri (2); terdiri dr protein lektin (disekitar bakteri, jumlah lebih
banyak)
 HEMOLYSIS ON BLOOD AGAR
 -hemolysis : partial lysis of RBC
• GREEN
• Non-groupable streptococci (S. pneumonia & S.
viridans)
• Viridans group
 -hemolysis : complete lysis of RBC  haemolyticus
group
• CLEAR ZONE
• Group A&B (S. pyogenes & S. agalactiae)
• Hemolyticus group
 -hemolysis : no lysis
• Group D (Enterococcus spp)
• Anhemolysis group
HEMOLYSIS ON BLOOD AGAR
 HEMOLYSIS ON BLOOD AGAR

-hemolysis

-hemolysis

-hemolysis
 OPTOCHIN TEST

Optochin
Resistant Optochin
(-) Sensitive
(+)
 BACITRACIN TEST

Bacitracin
resistant
(-)
Bacitracin
sensitive
(+)
 STREPTOCOCCUS PYOGENES

• Group A streptococcus
• Paling virulen dan paling sering
menyebabkan infeksi
• Masuk melalui kulit atau SPA
• Transmisinya melalui droplet atau kontak
langsung dengan luka
Cultural characteristics: On blood agar, after
overnight incubation, the colonies are small,
circular, low convex with an area of
β-haemolysis around them.
 VIRULENCE FACTORS

1. Adhesion
2. M protein
3. Capsule
4. Streptolysins
5. Pyrogenic exotoxins
 CLINICAL SIGNIFICANT

1. Acute pharyngitis/pharyngotonsilitis
2. Impetigo
3. Erysipelas
4. Puerperal sepsis
5. Scarlet Fever
6. Acute glomerulonephritis
7. Streptococcal toxic shock syndrome
Pharyngitis Impetigo

Erysipelas Scarlet Fever

 STREPTOCOCCUS PNEUMONIAE

• Commonly called Pneumococcus

• Diplococcus forming
• Oropharyngeal flora (5-70% of the
population)
• Genetically closely related to the
commensal S. mitis and S. oralis
 VIRULENCE FACTORS

1. Capsule
2. Pneumolysin
3. Autolysin
 CLINICAL SIGNIFICANCE

1. Acute bacterial pneumonia

2. Otitis media
3. Bacterimia/sepsis
4. Meningitis
Otitis Media Meningitis
 SOAL BONUS
1. SIAPAKAH NAMA CAPRES BEM FK UNS?
 SOAL BONUS
2. KAPAN PEMILU KBM FK UNS DILAKSANAKAN?
 THANK YOU

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