Geetanjali kumari
8th semester
Ranchi veterinary collage
Lipid profile or lipid panal is a panal of blood
tests that serves as an initial screening tool
for abnormalities in lipids,such as cholesterol
and triglyceride.
Lipid panal test can determine approximate
risks for cardiovascular disease,certain forms
of pancreatitis,and other diseases.
The lipid profile typically includes:
Low density lipoprotein{LDL}
High density lipoprotein{HDL}
Triglycerides
Total cholesterol
Very low density lipoprotien{VLDL}
Cholesterol:HDL ratio
o Lipid disorders are problems with the various
forms of fat that are carried in the
bloodstream.They include low density
lipoproteins(LDL),high density lipoproteins and
triglycerides.
o Lipids donot dissolve in water.For cholesterol
and fatty acids to be carried in the blood and
used in cells, the body use a kind of protein
called apoproteins to transport the lipids through
the blood and into the cells.These protein bound
fats are called lipoproteins.
o Each lipoprotein contains cholesterol,cholesterol
esters,triglycerides,phospholipids,vitamines and
apoproteins.
High density lipoprotein:
HDL is the smallest of the lipoprotein particles.It is the
densest because it contains the highest proportion of
proteins to lipids.
HDL transports cholesterol mostly to the liver or
steroidogenic organs such as adrenals,ovary and
testes by both direct and indirect pathways.
HDL removed by HDL receptors such as scavenger
receptor BI, which mediate the selective uptake of
cholesterol from HDL.
Low density lipoprotein:
Low density lipoproteins transports cholesterol from
the liver to the tissues of the body.It is called bad
cholesterol because it takes cholesterol to arteries.
MAJOR LIPOPROTIENS MINOR LIPOPROTEINS
CHYLOMICRONS INTERMEDIATE
VERY LOW DENSITY DENSITY
LIPOPROTEINS(V.L.D.L LIPOPROTEINS
) LIPOPROTEIN (a)
LOW DENSITY LIPOPROTEIN(x)
LIPOPROTEINS(L.D.L) Beta –V.L.D.L
HIGH DENSITY
LIPOPROTEINS(H.D.L)
Apolipoproteins are proteins that bind lipids
to form lipoproteins .They transport lipids in
blood,cerebrospinal fluid and lymph.
FUNCTION
They are cofactors various enzymes
Ligands for interaction with lipoprotein
receptors in tissues
Act as ligand for cell surface receptor
There are multiple classes for
apolipoproteins and several sub classes:
Apoliprotein A(APOAI,APOA2,APOA4,APO5)
Apolipoprotein B(apoB48, apoB100)
Apolipoprotein C (apo c1,apo c2,apo
c3,apoc4)
Apolipoprptein D
Apolipoprotein E
Apolipoprotein H
Apolipoprotein L
o Monitoring and maintaining healthy lipid
levels in the blood is crucial to stay healthy.
o It is a good indicator of any cardiovascular
related disease.
o A lipid profile helps the doctor in formulating
a treatment plan.
o Screening for primary and secondary
hyperlipidemias
LDL-cholesterol Decreased in:
o Severe illness
o Certain drugs
o A beta lipoproteinemia
LDL-cholesterol increased in:
o Familiar hypercholesterolemia
o Familiar combined hyperlipidemia
o Diabetes mellitus
o Nephrosis
o Chronic renal failure
Triglyceride increased in:
o Familiar hypertriglyceridemia
o Von gierke disease
o Diabetes mellitus
o Hypothyroidism
o Nephrosis
o Chronic renal failure
o Drugs
o Serum cholesterol decreased in:
o Severe liver damage
o Hyperthyroidism
o Malnutrition
o Chronic anemia
o Infection
o Drugs
Serum cholesterol increased in:
o Primary hyperlipoproteinemia
o Secondary hyperlipoproteinemia
o Diabetes mellitus
o Hypothyroidism
o Hemodialysis
o Obstructive liver disease
o Chronic alcoholism
HDL Cholesterol decreased in:
o Stress
o Acute myocardial infarction
o Stroke
o Surgery
o Starvation
o Diabetes mellitus
o Liver disease
o Nephrosis
HDL Cholesterol increased in:
o Moderate consumption of alcohol,insulin.
o Hyper alpha lipoprpteinemia
o Hypo beta lipoproteinemia
Factors that affect lipid profile of blood:
Posture
Fasting
Biological variations(Age,sex,season,food intake)
Life style factor
Exercise
Menstrual cycle
Alcohal ingestion
Smoking
Anticoagulants
TG,HDL cholesterol,TC can be analysed in
frozen samples
Apolipoproteins can also be measured in
frozen sample
Serum/plasma must be stored at -70 deg if
stored for long time
For short storage the sample can be kept at -
20 deg.
1. TOTAL LIPID
2. SERUM TOTAL CHOLESTEROL
3. SERUM HDL-C
4. TC/HDL-C
5. SERUM TRIGLYCERIDE
6. SERUM PHOSPHOLIPID
7. ELECTROPHORESIS
1. Modified ABELL KENDALL METHOD
2. PRINCIPLE:
3. Cholesterol esters hydrolysed with alcoholic KOH
4. Unesterified cholesterols are extacted with
petroleum ether.
5. Then they are measured with LIEBERMANN
BURCHARD REAGENT.
6. BURCHARD REAGENT is:
Cholesterol + Sulphuric acid +Acetic anhydride =bluish
green solution.
Cholesteryl esters +h20...........cholesterol+
free fatty acids
Cholesterol +02...............cholesten-4en-
3one +h2o2
H202 +phenol+4-
aminoantipyrine.......quinoenimine +2h20
The absorbance of quinoenimine produced is
measured at 500nm.
Red complex form
PRINCIPLE
Serum TG are hydrolysed to glycerol and free
fatty acids by lipase
In presense of ATP and glycerokinase,
glycerol is converted to glycerol phosphate
which is then oxidise to yield h202
H202 react with ESPAS to form colored
complex, the intensity of which is measured
at 546nm.
*ESPAS:N ethyl N sulfopropyl m anizidine
HOMOGENOUS ASSAY
PRINCIPLE
The method depend on the properties of
detergent which solublizes only the HDL so
that HDL-C is released to react with the
cholesterol esterase and cholesterol oxidised
and chromogen to give color.
The intensity of color is formed proportional
to concentration of HDL in sample, the
absorbance of which is measured at 600 nm.
FRIDEWALD CALCULATION