NUCLEAR SPIN
The nuclei of some atoms have a property called “SPIN”.
Nuclear Spin
Quantum No 1/2 1 0 1/2 1 0 5/2 1/2
(I)
No. of Spin 2 3 0 2 3 0 6 2
States
+ +
-1/2
In a strong magnetic
unaligned
field (Bo) the two
spin states differ in
energy.
+1/2
aligned
Bo S
Absorption of Energy
quantized
Opposed
-1/2 -1/2
DE
DE = hn
Radiofrequency
+1/2 +1/2
Applied
Field
Bo Aligned
THE ENERGY SEPARATION DEPENDS ON Bo
- 1/2
DE = kBo = hn
degenerate
at Bo = 0
+ 1/2
Bo
increasing magnetic field strength
The Larmor Equation!!!
DE = kBo = hn can be transformed into
gyromagnetic
ratio g
frequency of gBg0
the incoming
nn == Bo
radiation that
will cause a 2p
2p
transition strength of the
magnetic field
w Nuclei precess at
frequency w when
RADIOFREQUENCY
placed in a strong
40 - 600 MHz magnetic field.
hn
NUCLEAR
MAGNETIC
If n = w then RESONANCE
energy will be
absorbed and NMR
the spin will
invert. S
Resonance Frequencies of Selected Nuclei
Isotope Abundance Bo (Tesla) Frequency(MHz) g(radians/Tesla)
1H 99.98% 1.00 42.6 267.53
1.41 60.0
2.35 100.0
7.05 300.0
Radiation
induces both
upward and
downward resonance
induced
transitions. emission
MAGNET MAGNET
~ 1.41 Tesla
N S (+/-) a few ppm
Probe
Fortunately, different types of protons precess at
different rates in the same magnetic field.
N Bo = 1.41 Tesla
hn
To cause absorption
60 MHz 59.999820 MHz of the incoming 60 MHz
the magnetic field strength,
Bo , must be increased to
S a different value for each
type of proton.
Differences are very small,
in the parts per million range.
IN THE CLASSICAL NMR EXPERIMENT THE INSTRUMENT
SCANS FROM “LOW FIELD” TO “HIGH FIELD”
LOW HIGH
FIELD FIELD
NMR CHART
DOWNFIELD UPFIELD
scan
NMR Spectrum of Phenylacetone
O
CH2 C CH3
FT-NMR
requires a computer
PULSED EXCITATION
N
n2
n1
O
BROADBAND
RF PULSE CH2 C CH3
contains a range
of frequencies n3
(n1 ..... nn)
S
All types of hydrogen are excited
simultaneously with the single RF pulse.
FREE INDUCTION DECAY
( relaxation )
n1
O
CH2 C CH3 n2
n3
n1 + n2 + n3 + ......
time
FOURIER TRANSFORM
A mathematical technique that resolves a complex
FID signal into the individual frequencies that add
together to make it. ( Details not given here. )
FT-NMR
computer
COMPLEX
SIGNAL n1 + n2 + n3 + ......
Fourier
Transform
individual
a mixture of frequencies
frequencies
decaying (with time)
converted to a spectrum
The Composite FID is Transformed into
a classical NMR Spectrum :
O
CH2 C CH3
signal enhanced
noise signal
1st pulse
2nd pulse
add many
pulses
noise is random
nth pulse etc. and cancels out