Influence of Different Nitrogen

Rich Supplements on the

Growth and Productivity of
Pleurotus djamor on Rice
Straw Substrate

Presented by: Kent Miko Manlangit

 Introduction
• Pink Oyster Mushroom (Pleurotus
djamor ) is an edible mushroom. It is
commonly called pink oyster or salman
pink oyster because of its pink
sporophore, large sized fruit bodies and
delicious flavor.
• Hence, the study will be conducted
to evaluate the effects of different
nitrogen rich supplements on the
growth and productivity of Pleurotus
djamor.
 Objectives of the Study
1.Evaluate the effects of different
nitrogen rich supplements on the growth
and productivity of Pleurotus djamor.
2.Identify the cost and benefit of
growing Pleurotus djamor as influenced
by the different nitrogen rich
supplements.
3.Identify the different contaminations
on the fruiting bags substrates.
 Scope and Limitation of the Study
• The study will focus on the growth and
productivity on Pleurotus djamor as
influenced by the application of (4)
different nitrogen rich supplements.
 Time and Place of the Study

• The study will be conducted at CMU

Mushroom Production House, Department
of Plant Pathology, Central Mindanao
University, Musuan, Maramag, Bukidnon
from February to April 2019.
 Materials and Method
Materials
• Fruiting bodies of • Cotton plugs
Pleurotus djamor • Steamer
• Rice straws • Rubber band
• Coffee pulps • Plastic cover
• Peanut pods • Alcohol
• Mungbean pods • Polypropylene
• Empty palm mills plastic bags
 Methods
Experimental Layout
This study will be conducted using
Complete Randomized Design (CRD) with
five treatments replicated three times.
The following treatment will be used:
T1—Control (rice straw alone)
T2- Rice straw + coffee pulps
T3- Rice straw + peanut pods
T4- Rice straw + mungbean pods
T5- Rice straw + empty palm mill
 Methods
• Isolation
• Grain Spawn Preparation and Spawning
• Substrate Preparation
• Filling and Compressing the Bags
• Inoculation of the Fruiting Bags
• Incubation of the Spawn by Treatment
• Harvesting
 Data to be Gathered
1. Days to Full Mycelial Colonization
The appearance of the heavy mycelial
growth per bag will be determined by counting
the number of days from incoculation to the
time that full mycelial growth is observed in the
fruiting bags.
2. Days to Fruiting Bodies Formation
This will be done by counting the number
of days from the time the spawn bag is opened
to the time when small white fruiting bodies
develop.
3. Days to Harvesting
This will be done by counting the
number of days from opening of the fruiting
bags to the first harvest of mature fruiting
bodies.
4. Cap diameter (cm) of Fruiting Bodies
Ten (10) freshly harvested mushroom
caps will be collected from each treatment
and each cap diameter will be measured.
5. Number of Fruiting Bodies Formed
This will be done by counting the
number of fruiting bodies formed per bags
per treatment
6. Fresh Weight of Mushroom Fruiting Bodies
Freshly harvested mushroom fruiting bodies
per treatment will be weighed until 5 weeks only
7. Percentage Contamination
The percent contaminated fruiting bags will
be determined using the formula shown below:

Number of contaminted bags

% Contamination= x 100
Number of bags incubated
Experimental Layout
T1R1 T2R2 T3R1

T2R3 T3R2 T4R2

T3R1 T4R1 T5R3

T4R2 T5R1 T1R2

T5R2 T1R3 T2R1