Kuliah 3 - Struktur Virologi Tumbuhan - 2017
Kuliah 3 - Struktur Virologi Tumbuhan - 2017
TUMBUHAN
By Irda Safni
StrukturVirus
• Size range –
– most <0.2 μm; requires electron microscope
StrukturVirus
Karakteristik skala
ukuran adalah 30-
100 nm.
Susunannya sangat
simetris
(Baker et al.)
Viruses
Susunan Partikel Virus
•mengandung DNA atau RNA
Streptococcus E. coli
•membentuk paket pelindung
• CUBIC (Icosahedral/polyhedral):
Spherical, amount of nucleic acid that
can be packaged is limited by the
number of capsomers and the size of
the viral particle
E.g. Papilloma virus
Bentuk Virus
• HELICAL:
Rod shaped, varying widths and specific
architectures; no theoretical limit to the
amount of nucleic acid that can be
packaged
Bentuk Virus
• BACTERIOPHAGE
Polyhedral-shape head cylindrical
tail leglike fibres
Cubic (Icosahedral)
• Susunan Icosahedral adalah struktur virus yang
khas
• Suatu cosahedron memiliki 20 sisi segitiga, 12
sudut, dan 5:3:2 axis simetri rotasi
Helical
• Organized around a single axis (the “helix axis”)
• Probably evolved along with other helical structures
like
DNA, -helix, etc.
• Allow flexibility (bending)
• Helical viruses form a closely related spring like
helix instead. The best studied: TMV but many
animal viruses and phage use this general
arrangement.
– Note-all animal viruses that are helical are
enveloped, unlike many of the phage and plant
viruses.
• Most helixes are formed by a single major protein
arranged with a constant relationship to each other
(amplitude and pitch).
Helical symmetry
energy-independent fashion
Karbohidrat
- Karbohidrat virus hadir sebagai oligosakarida, rantai sisi dari
glycoproteins, glycolipids, dan mucopolysaccharide.
- Komposisi karbohidrat sesuai dengan komposisi karbohidrat
sel inang. Tetapi biasanya glycoproteins
Mempunyai sebuah untaian N– atau O- glycosidic .
- Karbohidrat virus biasanya dijumpai di amplop.
- Beberapa virus kompleks mengandung internal glycoproteins
atau glycosylated capsid proteins.
BROME MOSAIC VIRUS
•Type member of the
Bromovirus genus, family
Bromoviridae
•Virions are nonenveloped
icosohedrals (T=3), 26 nm
in diameter, contain 22%
nucleic acid and 78% protein
•BMV genome is composed
of three positive sense RNAs
separately encapsidated
RNA1 RNA2 RNA3
RNA4
Francki, Milne & Hatta. 1985 Atlas of Plant Viruses, vol. I.