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PENGATURAN EKSPRESI GEN

PADA PROKARYOTIK

Ade P_R Kartika D_C Febrianti S_S


PENDAHULUAN
A. Latar Belakang

Informasi genetik suatu organisme


tersimpan dalam molekul DNA yang
terdapat di dalam inti sel. Untuk
mengekpresikan informasi genetik yang
dimilikinya, sel melakukan berbagai
proses yang meliputi transkripsi dan
translasi. Suatu sel hanya
mengekspresikan gen yang dibutuhkan
pada lingkungan tertentu. Sehingga
membutuhkan pengaturan.
B. Topik

1. Struktur Gen 2. Mekanisme


Prokaryotik Ekspresi Gen

3. Regulasi Ekspresi 4. Operon &


Gen Regulasi Operon
TRANSCRIPTION DNA

mRNA
Ribosome
TRANSLATION

Polypeptide

Prokaryotic cell
Proses Transkripsi

 Pembentukan mRNA
dgn menggunakan
DNA sbg pola cetakan
 Dimulai dari promotor
dan berakhir di
terminator.
 Dikatalisis ensim RNA
polimerase.
Dasar Regulasi Operon
Regulasi Operon Lac
Kontrol Negatif Operon Lac

Regulatory Promoter
gene

Operator

DNA

No
RNA
made

mRNA RNA
polymerase

Active
Protein repressor

Lactose absent, repressor active, operon off


lac operon

DNA lacZ lacY lacA

RNA
3¢ polymerase
mRNA mRNA 5¢

Protein -Galactosidase Permease Transacetylase

Inactive
Allolactose
repressor
(inducer)
Lactose present, repressor inactive, operon on
Kontrol Positif Operon Lac

Promoter

DNA lacl lacZ

CAP-binding site RNA Operator


polymerase
Active can bind
cAMP CAP and transcribe

Inactive lac
Inactive
repressor
CAP
Lactose present, glucose scarce (cAMP level high): abundant lac
mRNA synthesized
Kontrol Positif Operon Lac

Promoter

DNA lacl lacZ

CAP-binding site Operator


RNA
polymerase
can’t bind
Inactive
CAP Inactive lac
repressor

Lactose present, glucose present (cAMP level low): little lac


mRNA synthesized
Regulasi Operon Trp

Tryptophan Present

Regulator Gene Promoter Operator Attenuator Structural Genes


RNA Polymerase

NO TRANSCRIPTION

trpR mRNA

+ tryptophan
(corepressor)

TrpR protein TrpR aporepressor


(homodimer) + corepressor (can
bind to operator)
DNA

mRNA

Protein Active
repressor

Tryptophan
(corepressor)
Tryptophan present, repressor active, operon off
DNA

No RNA made

mRNA

Protein Active
repressor

Tryptophan
(corepressor)
Tryptophan present, repressor active, operon off
Tryptophan Absent

Regulator Gene Promoter Operator Attenuator Structural Genes


RNA Polymerase

TRANSCRIPTION
trpR mRNA

TrpR protein TrpR aporepressor


(homodimer) (cannot bind to
operator)
SIMPULAN