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Resistensi Osmotik

Disusun oleh :
Dwi Susanti ( 22018008 )
Rizka Putri T ( 22018032 )
Fadliah ( 22018012 )
Nur Aulia Farikha ( 22018028 )
Sekarwati ( 22018037 )
Sintya Pramedya U ( 22018038 )
Pendahuluan

• Pemeriksaan Resistensi Osmotik (Daya Tahan


Osmotik) atau Osmotic Fragility Test digunakan
untuk membantu dalammenentukan tipe-tipe dari
jenis anemia.
• Faktor utama yang mempengaruhi pemeriksaan
fragilitas osmotik (osmotic fragility) adalah:
a. bentuk dari eritrosit
b. permukaan eritrosit
c. Volume
d. fungsi dari membran eritrosit.
Pendahuluan

Peningkatan fragilitas osmotic bisa


ditemukan pada:
 Anemi hemolitik
 Hereditery sperocytosis
Pendahuluan

Penurunan fragilitas osmotik terjadi pada:


1. Penyakit hati
2. Spleenectomy
3. Sickle cell anemia
4. Anemi defisiensi Fe
5. Thallasemia
6. Polisitemia vera
7. Keadaan dimana banyak terdapat bentuk target sel
dari eritrosit.
Pemeriksaan Fragilitas Osmotik (Osmotic Fragility)
atau
Resistensi Osmotik

Tujuan : Untuk mengetahui ketahanan membrane eritrosit


terhadap larutan hipotonik

Metode : SANFORD

Prinsip : Eritrosit dimasukan ke dalam berbagai


pengenceran larutan NaCl 0,5% sehingga
eritrosit membengkak dan hemolisis.
Alat dan Bahan

Alat Bahan

 Darah EDTA (1 mL darah


 Tabung reaksi
vena : 10µL larutan EDTA
 Rak tabung 10% )
 Pipet tetes  Larutan NaCl 0,5%
 Aquades
CARA KERJA :
1. Tabung reaksi disiapkan sebanyak 12 ta
CARA KERJA 2.Ke dalam tabung tersebut dimasukkan 25
23, 22, 21, 20,19,18,17,16,15 (tetes) dan
Larutan NaCl 0,50%
01
Tabung reaksi disiapkan 3.Kemudian ditambahkan akuades 0 tetes,
sebanyak 12 tabung
3, 4, 5, 6, 7, 8, 9, 10 (tetes) dan 11 tetes
masing-masing tabung menjadi 25 tetes
02 Ke dalam tabung tersebut dimasukkan 25 (konsentrasinya menjadi 0,50% ; 0,48%
0,44% ; 0,42% ; 0,40% ; 0,38%
tetes, 24, 23, 22, 21, 20,19,18,17,16,15 (tetes)

dan 14 tetes Larutan NaCl 0,50%


Despite being red, ; 0,36% ; 0,34% ; 0,32% ; 0,30% dan 0,28
03 Mars is cold

Venus has a beautiful


04 name
CARA KERJA
4.Ke dalam masing-masing tabung ditambahkan
sebanyak 1 tetes darah EDTA
5.Masing-masing tabung dikocok perlahan- lahan
sampai merata
6.Tabung diinkubasi selama 2 jam dalam suhu
kamar
7.Hasil pemeriksaan dibaca mulai dari
terjadinya hemolisis dan hemolisis total (
resistensi minimal dan resistensi maksimal)
CARA KERJA
No 1 2 3 4 5 6 7 8 9 1 1 12
0 1
NaCL 2 2 2 2 2 2 1 1 1 1 1 14
0.5% 5 4 3 2 0 1 9 8 7 6 5
(tetes)
11
Akuad 0 1 2 3 4 5 6 7 8 9 1
es 0
(tetes)
0,
Konse 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0,
28
ntrasi 5 4 4 4 4 4 3 3 3 3 3
%
laruta 0 8 6 4 2 0 8 6 4 2 0
Pembacaan
 Perhatikan warna dan intensitasnya serta
endapan eritrosit yang ada pada dasar tabung.
 Hemolisis :
Peristiwa yang terjadi pada kondisi hipotonik,
eritrosit tidak mampu menahan tekanan
sejumlah air yang masuk sehingga membran
eritrosit pecah dan hemoglobin keluar mewarnai
larutan sekelilingnya menjadi berwarna merah.
Pembacaan
 Permulaan hemolisis :
Ditandai dengan terdapatnya cairan berwarna
merah dibagian atas pada tabung yang pertama
kali dan pada dasar tabung terdapat endapan
eritrosit.

 Hemolisis Total:
Ditandai dengan cairan seluruhnya berwarna
merah dan tidak ada gumpalan eritrosit di
dasar tabung
Pembacaan
 Resistensi Minimal:
Permulaan hemolisis dari eritrosit
dalam cairan dengan konsentrasi
NaCl tertinggi.
 Resistensi maksimum:
Hemolisis total dari eritrosit dalam
cairan dengan konsentrasi NaCl
tertinggi
NILAI NORMAL
 NILAI NORMAL :
 Resistensi maksimal : 0,32 % –
0,36%
 Resistensi minimal : 0,42 % –
0,46%
Hasil Pengamatan
Hasil Pengamatan :

1 2 3 4 5 6 7 8 9 1 1 1
0, 0, 0, 0, 0, 0, 0, 0, 0, 0 1 2
5 4 4 4 4 4 3 3 3 0, 0, 0
R%esist%ensi%Minim%al:(%0,42%%– %0,46%%) ,
Permulaan
0 8 hemolisis
6 4 dari
2 eritrosit
0 8 dalam
6 cairan
4 %deng%
3 3an %
2
konsentrasi NaCl tertinggi. % 2 0 8
Resistensi maksimal: (0,32 % – 0,36%)
Hemolisis total dari eritrosit dalam cairan dengan konsentrasi
NaCl tertinggi
“This is a quote. Words full of wisdom
that someone important said and can
make the reader get inspired.”

—Someone Famous
WHAT IS THIS TOPIC ABOUT?

Here you could give a brief description of


the topic you want to talk about. For
example, if you want to talk about Mercury,
you could say that it’s the closest planet to
the Sun and the smallest one in our Solar
System. It’s only a bit larger than our
Moon, and its name has nothing to do with
the liquid metal, since it was named after
the Roman messenger god, Mercury
DEFINITION OF CONCEPTS

MERCURY SATURN MARS VENUS


It’s the closest planet It’s the ringed one, Despite being red, Venus has a
to the Sun and the composed mostly of Mars is actually a beautiful name
smallest one in our hydrogen and helium cold place. It’s full of and is the second
Solar System iron oxide dust planet from
the Sun
FEATURES OF THE TOPIC

MARS JUPITER VENUS

Despite being red, Mars It’s a gas giant and the Venus has a beautiful
is actually a cold place. biggest planet in our name and is the second
It’s full of iron oxide Solar System. Jupiter is planet from the Sun. It’s
dust, giving the planet the fourth-brightest terribly hot, even hotter
its reddish cast object in the sky than Mercury
Remember that big
numbers grab a lot of
7.2M
attention

10K You can use them to


make an impression

In Spain, a comma is
used as a decimal
separator

33,400
PRACTICAL
USES OF THIS
SUBJECT

MERCURY VENUS

It’s the closest planet Venus has a beautiful


to the Sun name

SATURN MARS

Yes, this is the ringed Despite being red,


one Mars is cold
LESSON DEVELOPMENT

Mercury is the smallest


01 one

Saturn is the ringed


02 one

Despite being red,


03 Mars is cold

Venus has a beautiful


04 name
Despite being red, Mars is actually a
cold place. It’s full of iron oxide dust,
which gives the planet its reddish
cast

Insert your multimedia content here


DID YOU KNOW...?

MERCURY
Mercury is the closest
planet to the Sun

SATURN
Saturn is the ringed planet
and a gas giant

MARS
Despite being red, Mars is
a cold place
...and did you know this?

Despite being red, Mars is actually a


cold place. It’s full of iron oxide dust,
which gives the planet its reddish
cast

If you want to modify this graph, click on it, follow the link, change the data
and replace it here
PROBLEM SOLUTION

Mercury is the closest Jupiter is a gas giant


planet to the Sun and and the biggest planet
the smallest one in the in our Solar System. It’s
Solar System. It’s only a also the fourth-brightest
bit larger than our Moon object in the sky
OVERVIEW DIAGRAM

PART 1 PART 2

NOVEMBER DECEMBER JANUARY FEBRUARY MARCH

LESSON 1 LESSON 2 LESSON 3 LESSON 4 LESSON 5

Mercury is Despite being Venus is the Saturn is the Neptune is


the closest red, Mars is a second planet ringed planet the farthest
planet to the cold place from the Sun and a gas planet from
Sun giant the Sun
EXERCISE

● Mercury is the closest planet to the


?
Sun, but does its name have
anything to do with the liquid metal?

● Contrary to popular belief, no. The


truth is that this planet was named
after the Roman messenger god,
Mercury
?
ASSIGNMENT

Mercury is the closest planet to the Sun,


and Neptune is the farthest one. Calculate
the distance between these two planets

?
OTHER CONCEPTS

MERCURY SATURN VENUS

It’s the smallest planet This is the ringed Venus has a beautiful
in our Solar System and planet, composed name and is the second
the closest to the Sun mostly of hydrogen and planet from the Sun
helium

MARS JUPITER NEPTUNE

Despite being red, Mars Jupiter is a gas giant Neptune is the farthest
is actually a cold place. and the biggest planet planet from the Sun and
It’s full of iron oxide dust in our Solar System the fourth-largest
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