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Organizing Science

Research Papers (2)




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Acuolvirus expression system has been extensively adopted
in protein expression in recent years.
However, the conventionally adopted procaryote expression
system fails to compare posttranslational modification with the
baculovirus expression system.
Posttranslational modification of the baculovirus
expression system is better than that of the procaryote expression
system. While producing proteins such as those found in humans, this
baculovirus expression system requires less time than the mammalian
expression system does.
The inability to use the baculovirus expression
system to produce proteins requires use of the mammalian expression
system to produce human proteins, which is inefficient and expensive.
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Based on the above, by using the baculovirus expression system, we
should attempt to achieve expression of the trypsin inhibitor and, in doing so,
use this protein to inhibit cancer. Posttranslational modification of the
baculovirus expression system is better than that of the procaryote expression
system, especially in terms of the protein quality because it closely resembles
that of humans.
To do so, a trypsin inhibitor can be produced using a
baculovirus expression system. Effectiveness of the trypsin inhibitor can then
be confirmed by western blotting. Next, a cancer cell can be terminated via the
trypsin inhibitor.
As anticipated, the baculovirus expression system can be used as
an expression trypsin inhibitor to promote anticancer activities.
Results of this study can provide valuable insight into how the
baculovirus expression system can be used to achieve the expression protein,
given that this protein closely resembles that found in humans. The baculovirus
expression system more closely resembles a human protein than the
procaryote expression system does. In practice, although this expression may
not be better than mammalian expression, the baculavirus expression system
requires a shorter time.
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As a disease commonly spread through sexual transmission,
syphilis also occurs through blood transfusions, exchange of body
fluids or through a mothers placenta to the fetus. Treponema pallidum
is the most causitive gent of syphilis.
A typical diagnosis method involves preliminary screening
nontreponemal tests, e.g., venereal disease research laboratory
(VDRL) and rapid plasma reagin (RPR) tests. As the basic syphyilis
screen order, VDRL and RPR tests use a patients serum for
testing. A situation in which a patient has monocyte infection or
malaria infection can often interfere with the test results, not only
yielding a probability of false positive of around 20% but also causing
a high degree of non-specificity. Another typical diagnosis method
involves performing a confirmation test, commonly refered to as
treponemal tests, which include Treponema pallidum
hacmagglutination (TPHA) and Flourescent Treponemal Antibody
Absorption (FTA-ABS). However, these methods are expensive and
inefficient.
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For instance, conventional determination
methods easily miss a patients latent phase and have a
poor diagnosis accuracy of only around 80% during
examination.
The inability to resolve the limitations of
conventional methods, i.e. the high non-specificity,
expensiveness and inefficiency of VDRL, RPR, TPHA and
FTA-ABS, will require not only more personnel to diagnose
a disease during a laboratory examination, but also a
higher cost per examination. Moreover, a disease in
patients in the latent phase is difficult to detect and will
likely spread.

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Based on the above, we should develop a syphilis diagnostic kit,
capable of detecting in only one step the anti-treponema specific antibodies in
a patients serum or plasma binding to the antigen.
To do so, the serum samples of syphilis patients can be
analyzed. An experiment involving the syphilis diagnostic kit can then be
performed with the Treponema pallidum antigen, TpN 15, TpN17 and TpN
47. Next, the gene recombinant method can be adopted to produce
traponemal antigens. Additionally, the fusion protein can be used to achiee
protein expression in the E.coli expression system. Moreover, a syphilis
recombinant system can be established usingrecombinant technology.
As anticipated, analysis results can indicate that a fusion protein
can be formed using TpN 15, TpN 17 and TpN 47. That protein can then be
used by gene recombinant technology to achieve procreation on a large
scale. Hopefully, product procreation on a large scale can elevate the
sensitivity of detecting syphilis disease to within an accuracy of 100%.
With a high degree of accuracy and specificity, the proposed
syphilis diagnostic kit can be applied for use in genetic engineering, protein
construction and reagent key technology. Moreover, the diagnostic kit is highly
promising for commercialization if the reagent can be developed successfully,
thus reducing the diagnostic time while increasing its accuracy significantly.
Further details can be found at
http://www.chineseowl.idv.tw

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