Organizing Science

Research Papers (5)

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As a disease commonly spread through sexual transmission, syphilis
also occurs through blood transfusions, exchange of body fluid or through a
mothers placenta to the fetus. Notably, treponema pallidum is the most
common causative agent of syphilis.
A typical diagnosis method involves performing initial screening
nontreponemal tests, e.g., venereal disease research laboratory (VDRL) and
rapid plasma reagin (RPR) tests. As the basic syphilis screen order, VDRL and
RPR tests use a patients serum for testing. A situation in which a patient has
monocyte injection or malaria infection can often interfere with the test results,
not only yield a probability of false positive of round 20%, but also cause a high
degree of non-specificity. Another typical diagnosis method involves
performing a confirmation test, commonly referred to as treponemal tests,
which include treponema pallidum hacmagglutination (TPHA) and fluorescent
treponemal antibody absorption (FTA-ABS). However, these methods are
expensive and inefficient.
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For instance, conventional determination
methods easily miss a patients latent phase, and have a
poor diagnosis accuracy of only around 80% during
examination.
The inability to resolve the limitations of
conventional methods, i.e. the high non-specificity,
expensiveness and inefficiency of VDRL, RPR, TPHA, and
FTA-ABS, will require not only more personnel to diagnose
a disease during laboratory examination, but also a higher
cost per examination. A disease in patients in the latent
phase is difficult to detect and will likely to spread.

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Based on the above, we should develop a syphilis diagnostic kit, capable of
detecting in only one step the anti-treponema specific antibodies in a patients serum or
plasma binding to the antigen.
To do so, the serum samples of syphilis patients can be analyzed. An
experiment involving the syphilis diagnostic kit can then be performed with the treponema
pallidum antigent TpN 15.TpN17 and TpN 47. Next, the gene recombinant method can
be adopted to produce treponemal angiens (TpN 15.TpN17 and TpN 47 fusion protein).
Additionally, the fusion protein can be used to achieve protein expression in the E.coli
expression system. Moreover, a syphilis recombinant system can be established using
recombinant technology.
As anticipated, analysis results can indicate that a fusion protein can be
formed using TpN 15.TpN17 and TpN 47. That protein can then be used by gene
recombinant technology to achieve procreation on a large scale. Hopefully, product
procreation on a large scale can elevate the sensitivity of detecting syphilis disease to
within an accuracy of 100%.
With a high degree of accuracy and specificity, the syphilis diagnostic kit
developed herein can be applied for use in genetic engineering protein construction and
reagent key technology. Moreover, the proposed diagnostic kit is highly promising for
commercialization if the reagent can be developed successfully, thus reducing the
diagnostic time while increasing its accuracy significantly.
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Curcumin can inhibit the activation of cancer
cells in humans.
However, merely consuming plenty of natural
foods such as vegetables and fruits does not ensure a
concrete expression to circumvent the activation of cancer
cells.
For instance, the inability to consume
10uM of curcumin consistently will not prevent the
activation of cancer cells.
The inability to consume adequate
amounts of curcumin to inhibit cancer cell growth makes it
impossible to ensure sustained growth.
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Based on the above, we should develop a determination
method to identify various concentrations of curcumin that would inhibit
cancer cell growth, enabling us to determine the most efficacious
concentration.
To do so, carcimoma macrophase culture can be
made for high glucose and low glucose. The survival rate of the MTT
conversion cell can then be analyzed by adding various concentrations
of curcumin. Next, subsequent MTT data can provide evidence of O.D
550nm of ELISA.
As anticipated, analysis results can indicate that various
concentrations of curcumin can inhibit cancer cell growth.
Importantly, the proposed determination method
significantly contributes to efforts to elucidate the potential role of
curcumin in decreasing the incidence of cancer.
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