“UJI ANTIBAKTERI JAMUR ENDOFIT DARI TUMBUHAN MANGROVE

Sonneratia alba YANG TUMBUH DI PERAIRAN PANTAI

TANAWANGKO “
Yohanis Nawea, Remy E.P Mangindaan, Robert A.Bara
Jurnal Pesisir dan Laut Tropis
Vol.1 (1) 2017

ANDI WIJAYA
08051181823011
KELAS A
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DOSEN PENGAMPUH:
Dr. M.HENDRI,. S.T,.M.Si.
01 Pendahuluan

02 Metodologi

03 Hasil dan Pembahasan

04 Kesimpulan
 PENDAHULUAN
Jamur endofit merupakan jamur yang hidup di dalam jaringan tumbuhan tanpa memperlihatkan
timbulnya Isolasi jamur endofit Sampel daun, ranting dan akar mangrove S. alba asal Perairan
Tanawangko penyakit pada tumbuhan tersebut. Jamur ini memiliki potensi yang besar untuk
dieksploitasi dan menghasilkaN senyawa-senyawa alami baru yang bermanfaat di bidang medis,
pertanian, dan industri.
Resistensi antibiotik merupakan problematika di bidang kesehatan. Berbagai jenis kuman
patogen berkembang menjadi resisten terhadapsatu atau beberapa jenis antibiotika.Terdapat dua
hal mendasar terkaitdengan terjadinya resistensi bakteri terhadap antibiotik yaitu kemampuan
bakteri untuk membentuk pertahanan diri terhadap antibiotik secara cepat mati dan
ketidaktepatan dosis dalam tindakan.
Mangrove Sonneratia alba berkemampuan untuk menghambat bakteri karena sejak beberapa
abad yang dinokulasi ke dalam media agar baru tanpa pemberian antibiotik. Setelah
pertumbuhan misilia mangrove sebagai obat-obatan (getah untuk antifertilitas atau mencegah
kehamilan, salep dari biji untuk obat penyakit cacar atau penyembuh luka. Karena itu sangat
dimungkinkan jamur endofit dari mangrove S. alba ini berpotensi untuk dikembangkan jamur
endofit mencapai bagian dasar sebagai bahan obat-obatan.
 METODOLOGI
Skrining Kultivasi dan
ISOLASI JAMUR ekstraksi Partisi
aktivitas
jamur pada
antibotik media nasi
Sampel daun, ranting
dan akar mangrove S. Miselia jamur endofit Partisi dari ekstrak kasar
alba di potong- yang sudah tumbuh Kultur jamur pada jamur dilakukan pada
potong 5 cm dan diambil sebagian media nasi secara ekstrak yang menunjukan
dicelupkan ke dalam dengan static selama 10 hari aktivitas antibakteri yang
etanol 70% selama menggunakan pada suhu ruang. Kuat.
sedotan steril dan Setelah misilia jamur
30 detik. Setelah
dipindahkan ke endofit mencapai
itudi potong ladi
media kombinasi bagian dasar
menjadi 1 cm yang sudah diolesi Erlenmeyer dilakukan
menggunkan gunting bakteri uji. Lalu ekstraksi dengan
steril. Selanjutnya cawan petri di menambahkan etanol
potongan tadi di inkubasi selama 1-2 95% samapai nasi
masukan kedalam x 24 hari. Setelsh itu terendam, lalu diaduk
media dengan tiap diamati aktivitas dengan shaker selama
cawan berisi 4 antibiotic terhadap 24 jam. Setelah itu
potong sampel, lalu S.aureus dan E.coli. rendaman di saring dan
di simpan di suhu di evaporasi 40
kamar 25 °C). ° C.
Your Picture Here

HASIL DAN PEMBAHASAN

1. Hasil pengujian awal isolat jamur

pada bakteri S. aureus dan E. coli

2.Ekstraksi dari Jamur Endofit pada

Media Nasi

3.Pengujian aktivitas antibakteri dari

ekstrak

4. Partisi dan uji aktivitas antibioti

Your Picture Here

Hasil pembiakan jamur endofit

yang berasal dari akar, ranting dan
daun mangrove Sonneratia alba yang
diambil dari lokasi pantai Tanawango
menghasilkan 9 spesies jamur yaitu
tiga dari isolat daun, dua dari isolat
batang dan empat dari isolat akar.
Pada bagian tanaman yang berbeda
dapat diisolasi jamur endofit yang
bervariasi/berbeda-beda bentuk koloni
dan perbedaan warna pada miselia.
Isolat jamur yang tumbuh pada bagian
akar memiliki karakteristik meselia
berwarna hitam dan kuning, sedangkan
pada bagian ranting isolat jamur
memiliki miselia dengan karakteristik
warna kuning dan putih dan pada
bagian daun berwarna kuning dan
putih. Pada bagian isolat daun dan
isolat akar ada isolat yang memeiliki
kesamaan warna dan karakteristik
bentuk yang sama dapat dilihat pada
 Sembilan isolat jamur endofit telah
diisolasi mangrove S. alba yang

KESIMPULAN tumbuh di Perairan Pantai

Tanawangko
Minahasa..
Kabupaten

Pengujian awal menunjukkan lima Isolat

jamur memiliki aktivitas antibakteri
terhadap isolat klinik S. aureus dan E.
01
coli. Tiga isolat akar (PTWSAA 1.1, 1.2
dan 1.3) memperlihatkan aktivitas yang
kuat pada kedua bakteri uji di atas. . 04
Induksi bakteri S. aureus pada kultur
isolat jamur endofit PTWSAA 1.2 tidak
memberikanpengaruhterhadap
aktivitas antibakteri jamur tersebut.
03

Pengujian aktivitas antibakteri setiap

fraksi isolat jamur endofit yang diuji
pada penelitian ini hanya fraksi etanol
02
yang memperlihatkan aktivitas
penghambatan, fraksi air dan
hambat 05
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