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Uji toksisitas akut dan subakut dari ekstrak

Pegagan (Centella asiatica Linn) pada


percobaan tikus

Oleh Syahrul Umam


11161114

Pembimbing
Utama : Dr. Yani mulyani, M.Si., Apt
Serta : Dr. Patonah, M.Si., Apt
outline
01 Pendahuluan

02 Tinjauan tanaman

03 Tinjauan uji toksisitas

04 Metode penelitian

05 Daftar pustaka
BPOM, 2005
Obat tradisional telah digunakan secara empiris untuk
L pengobatan berbagai penyakit
B
A E Hasimun et al 2018, kumar et al 2014
T L Pegagan memiliki berbagai aktivitas farmakologi antara
lain mengatasi kekakuan arteri, Antimikroba dll.
A A
K
R A Uji toksistas
Pencegahan terjadinya efek toksik, gangguan fisiologis
N dan kerugian akibat konsumsi pegagan

G
Metode penelitian
14 hari

Akrimatisasi Pengamatan
Akut toksisitas
Hewan OECD 420

Penentuan
LD 50
Penyiapan Ekstrak
bahan
Uji toksisitas
pegagan
28 hari

Sub kronik
Determinasi OECD 407 Pengamatan
dan penyiapan toksisitas
ekstrak
Pengamatan patologi
dan histologi
Prosedur uji toksisitas akut
10 ekor tikus

Diadaptasi 7 hari

uji pendahuluan dosis 300mg, 2000mg

uji utama dosis 5000mg

Diamati 14 hari

Dikorbankan

Penentuan derajat toksisitas


Prosedur uji toksisitas subkronik
29Dikorbankan
Diamati
diamati scr
setiap
Di induksi patologi dan
Dibuat harinya
28 hari histopatologi
menjadi 4
kelompok
uji
diaklim
atisasi

Hewan
uji
kontrol 50 mg/kg 100 mg/kg 200 mg/kg
bb bb bb
Daftar pustaka
Arora, Renu, Ritesh Kumar, Amit Agarwal, K. H. Reeta, and Y. K. Fong, Lai Yen, Chin Theng Ng, Yoke Keong Yong, Nazrul
Gupta. 2018. “Comparison of Three Different Extracts of Centella Hakim, and Zuraini Ahmad. 2018. “PT US CR.” Vascular
Asiatica for Anti-Amnesic, Antioxidant and Anticholinergic Pharmacology #pagerange#.
Activities: In Vitro and in Vivo Study.” Biomedicine and Hasimun, Patonah, Yani Mulyani, Agus Sulaeman, Dewa Ayu,
Pharmacotherapy 105(November 2017):1344–52. and Embas Saraswati. n.d. “Asian Journal of Biological
Baradaran, Azar, Hamid Nasri, and Mahmoud Rafieian-Kopaei. Sciences Research Article Prevention of Hypertension and
2014. “Oxidative Stress and Hypertension: Possibility of Arterial Stiffness by Combination of Centella Asiatica and
Hypertension Therapy with Antioxidants.” Journal of Research in Curcuma Longa in Rats.”
Medical Sciences: The Official Journal of Isfahan University of Herbal, Obat and Terstandar Dan. 2005. “Badan Pengawas
Medical Sciences 19(4):358. Obat Dan Makanan Republik Indonesia.” 1–14.
Besung, Kertanengah. 2009. “Pegagan (Centella Asiatica) Hodgson, E., Levi P. E. 2010. A Textbook of Modren Toxicology.
Sebagai Alternative Pencegahan Infeksi Pada Ternak.” Jurnal IV.
Penelitian 2(2):61–67. Hoff, Janet. 2000. “Methods of Blood Collection in the Mouse.”
BPOM. 2014. “Peraturan Kepala Badan Pengawas Obat Dan Lab Animal.
Makanan Republik Indonesia No. 7 Tahun 2014 : Pedoman Uji James, Jacinda and Ian Dubery. 2011. “ Identification and
Toksisitas Nonklinik Secara In Vivo.” (875). Quantification of Triterpenoid Centelloids in Centella Asiatica
Duggina, Pragathi, Chandra Mouli Kalla, Seshadri Reddy (L.) Urban by Densitometric TLC .” Journal of Planar
Varikasuvu, Suman Bukke, and Vijaya Tartte. 2015. “Protective Chromatography – Modern TLC 24(1):82–87.
Effect of Centella Triterpene Saponins against Kementerian, Badan Litbangkes. n.d. “No Title.”
Cyclophosphamide-Induced Immune and Hepatic System Kumar, Vi., Sharma, A., Machawal, L., Nagarajan, K. dan
Dysfunction in Rats: Its Possible Mechanisms of Action.” Journal Siddiqui, S. A. 2014. “Effect of Centella Asiatica against Anti-
of Physiology and Biochemistry 71(3):435–54. Tuberculosis Drugs-Induced Hepatotoxicity : Involvement of
Mitochondria and Oxidative Stress.” The Journal of
Phytopharmacology 3(5):310–15.
Daftar pustaka

kumar, V. Siv., A. Mohamed Sadiq, and S. Divya Bharathi. 2018. Solet, Simón- and 1998 Ramiasa, Cosson, & Guignard. 1998.
“Hepatoprotective Activity of Centella Asiatica Linn. against Paracetamol “VI Centella Asiatica ( L .) Urban . ( Pennywort ): Cell Culture ,
Induced Liver Damage in Experimental Animals.” Emergent Life Production of Terpenoids , and 1 Introduction.” 41.
Sciences Research 4(1):19–26. Subban, Ravi, A. Veerakumar, R. Manimaran, K. M. Hashim, and
Mayet, Jamil and Alun Hughes. 2003. “Pathophysiology in Indira Balachandran. 2008. “Two New Flavonoids from Centella
Hypertension.” Heart Journal 89:1104–1109. Asiatica (Linn.).” Journal of Natural Medicines 62(3):369–73.
Nadia, Nur, Mohd Razali, Chin Theng Ng, Lai Yen Fong, Health Yingchun, Li, Wang Huihan, Zhang Rong, Zhang Guojun, Yang
Sciences, Universiti Tunku, and Abdul Rahman. 2019. “Cardiovascular Ying, and Liu Zhuogang. 2019. “Antitumor Activity of Asiaticoside
Protective Effects of Centella Asiatica and Its Triterpenes : A Review.” Against Multiple Myeloma Drug-Resistant Cancer Cells Is
1203–15. Mediated by Autophagy Induction, Activation of Effector
Narvariya, Umesh, Neetesh K. Jain, and Anis Shaikh. 2019. “Journal of Caspases, and Inhibition of Cell Migration, Invasion, and STAT-3
Drug Delivery and Therapeutics Anti-Hyperglycemic & Anti- Signaling Pathway.” Medical Science Monitor 25:1355–61.
Hyperlipidemic Activity of Leaves of Centella Asiatica Linn . in Diabetic
Rats.” 8:150–54.
Orhan, Ilkay Erdogan. 2012. “Centella Asiatica (L.) Urban: From
Traditional Medicine to Modern Medicine with Neuroprotective
Potential .” Evidence-Based Complementary and Alternative Medicine
2012:1–8.
Rahayu, M. &. moch firman. 2018. Toksikologi Klinis.
Sabaragamuwa, Rasangani, Conrad O. Perera, and Bruno Fedrizzi.
2018. “Centella Asiatica (Gotu Kola) as a Neuroprotectant and Its
Potential Role in Healthy Ageing.” Trends in Food Science and
Technology 79(April):88–97.
Thank you

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