Presented By:
M.C.Mohamed Zakeel
H.M.P.S. Kumari, U.N.D. Silva, W.M.
Abayarathne, D.S. De Z. Abeysiriwardena,
I.W.M.K.N.K. Yatawara and D.N. Sirisena
N6 medium
Different combination & concentration of
growth regulators
Anthers removed from spikelets
100 anthers per each petridish
25 petridish per each medium
Incubated under dark at 25oC
Treatment Sugar type Growth regulators (mg/l)
Sucrose % Maltose % 2,4 D KN NAA BAP
Callus induction
N6-1 6 2 0.5 - -
N6-2 6 - 0.5 2 -
N6-3 6 2 1 - -
N6-4 6 - 1 2 -
N6-5 6 2 0.5 2 -
N6-6 6 2 1 2 -
Plant regeneration
MS1 3 - 0.5 1 1
MS2 3 - 0.5 1 2
MS3 3 - 2 1 0.5
MS4 3 - 2 1 2
Partial desiccation of calli & plantlet
regeneration
Bg 750 39 - 26 -
Bg 300 7 - 5 -
Bg 94-1 7 - 24 5 (20.8)
At 401 17 - 15 7 (46.7%)
Bw 400 10 - 15 4 (16.7%)
Nonabokra 12 - 11 -
Variety MS3 MS4
Bg 750 41 - 10 -
Bg 300 3 - 7 -
Bg 94-1 17 - 10 -
At 401 36 - 33 -
Bw 400 15 - 8 2 (25%)
Nonabokra 9 - 9 -
Cont…