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Crispino, Jhezelle

Dianalan, PZU Nur-Nasrin R.


Pascual, Jessica C.

Used to measure concentrations of


absorbing
materials
based on
developed calibration curves of the
material.

Relates the absorbance, the concentration


of the absorbing species and the path length

Absorbance

varies linearly with both the cell


path length and the analyte concentration
A = bc

Limitation:

Applicable
samples (<0.01M)

only

for

dilute

A quantitativemeasure of the strength of anacidin solution

The larger the value of pKa, the smaller the extent of


dissociation

Aweak acidhas a pKavalue in the approximate range 2 to 12 in


water

Acids with a pKavalue of less than about 2 are said to be strong


acids

Weak acids and bases used for testing pH


levels in water solutions

Displays different colors at different pH


because of the differences between the
electronic spectra of its conjugated acid and
base

An

expression which relates the pH, the pKa


and the concentrations of the acid and base
species in a buffer solution

pH = pKa - log (cA/cB)

pH = pKa - log ([HIn]/[In-])

1)

To utilize UV-Vis spectrophotometry in the


determination of the pKa of the assigned
acid-base indicator.

Table 1. Theoretical Data for UV-VIS Spectrophotometry ( = ~435nm)

pH

(nm)

Abs

435.469

0.054

3.2

435.4

0.059

3.4

435.4

0.0585

3.6

435.2349

0.0545616

3.8

435.2359

0.04931

435.2349

0.03577

4.25

435.2349

0.02738643

4.32

435.2349

0.02215453

4.5

435.2349

0.01443309

4.66

435.2349

0.00869336

~10

435.2349

-0.0033752

Table 1. Theoretical Data for UV-VIS Spectrophotometry ( = ~590nm)

pH

(nm)

Abs

590

3.2

590.3073

0.029

3.6

590.1566

0.06799

3.8

590.1566

0.09462508

590.1566

0.129184

4.25

590.1566

0.148853

4.32

590.1566

0.1613375

4.5

590.1566

0.18025143

4.66

590.1566

0.1915218

~10

590.1566

0.2649229

Accounts

for contributions in absorbance of


other species.
Eg: at = 590nm, assume only basic form is
present but acidic species may also absorb.
To correct, subtract the minimum
(background absorbance) from measured
absorbance.

Table 1. Theoretical Data for UV-VIS Spectrophotometry ( = ~435nm)

pH

Abs

A-AIn

AHIn -A

Log [(A-AIn ) / (AAHIn


)]

0.054

-0.04531

3.2

0.059

0.005

-0.05031

3.4

0.0585

0.0045

-0.04981

3.6

0.0545616

0.000562

-0.04587

3.8

0.04931

-0.00469

-0.04062

-0.93753115

0.03577

-0.01823

-0.02708

-0.1718081

4.25

0.02738643

-0.02661

-0.01869

0.153422503

4.32

0.02215453

-0.03185

-0.01346

0.373964854

4.5

0.01443309

-0.03957

-0.00574

0.838440672

4.66

0.00869336

-0.04531

~10

-0.0033752

-0.05738

y=2.3726x-9.8518
pKa

= -(-9.8518/2.7326)
pKa= 3.605

Table 1. Theoretical Data for UV-VIS Spectrophotometry ( = ~590nm)

pH

Abs

A-AIn

AHIn -A

Log [(A-AIn ) / (AAHIn


)]

-0.26492

3.2

0.029

-0.23592

-0.029

0.910372

3.6

0.06799

-0.19693

-0.06799

0.461873

3.8

0.09462508

-0.1703

-0.09463

0.255203

0.129184

-0.13574

-0.12918

0.021496

4.25

0.148853

-0.11607

-0.14885

-0.10804

4.32

0.1613375

-0.10359

-0.16134

-0.19244

4.5

0.18025143

-0.08467

-0.18025

-0.32814

4.66

0.1915218

-0.0734

-0.19152

-0.41652

~10

0.2649229

-0.26492

y=

-0.9044x+3.7303
pKa = -(3.7303/-0.9044)
pKa= 4.12

In order to determine the pka of an acid base


indicator solution is the reliance to
Henderson Hasselbach equation.
Ratio of acid and basic form of indicator is
dependent on the pH based spectroscopic
behavior of the solution.
The large % error is due to instrumental
reasons.

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