session
Light
microscopy of
WBC and
Erythroid cells
Eldad J.Dann MD
WBC Differential Cytochemistry
Comparison of Wright Stain and Peroxidase Stain
E M
L
L = Lymphocyte
N B = Basophil
N
M M = Monocyte
B
N
N = Neutrophil
N
L N E = Eosinophil
L
1
5
SCATTER (Size)
3
4
ABSORBANCE
eos
Monocytes
lymphocytes
Neutrophils
MN PMN
MCV mean corpuscular volume 80-94 fl (10-15( L
MCH mean corpuscular hemoglobin 27-31 pg (10-12(gm
MCHC mean corpuscular hemoglobin concentration
32-36 gm/dl
% RDW- 11.5-14.5
Example: Hct 45% RBC 5x106/ul Hb 15 gm/DL
MCV= 450(L)x1000/ 5 X1012(L)= 90 fL
MCH= 150gm/L /5 X1012(L)= 30 pg
MCHC= MCH /MCV =(15 gm/DL)/ 0.45= 33g/DL
RBC Analysis
High angle detector
(5o - 15o)
670nm
Laser
Diode
120fL
60fL
28g/dL 41g/dL 0 28 41 50 g/dL
Micro Hgb Concentration (HC)
Hypo Hyper
Hgb Concentration (g/dL)
0 100 pg
Hgb Content (CH)
Normal Sample: MCV > 80 fL
Sickle Cell Anemia
A B C D E
צביעת משטחים של תאי דם במעבדה ההמטולוגית
מרכז רפואי רמב"ם
שימוש במכשיר:
Blood 2007;109:858