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ACHMADIN LUTHFI BIOINDUSTRI BPPT

Teknik immobilisasi enzim untuk peningkatan stabilitas lipase dan aplikasinya pada industri pangan berbasis CPO

Pengolahan CPO menjadi CBE

Pendahuluan
Indonesia penghasil kelapa sawit terbesar didunia Tahun 2007: 17 juta ton CPO Tahun 2010: 23.2 juta ton CPO 70% diekspor Nilai tambah produk turunan CPO CPO Cocoa butter Equivalent (CBE)

Aplikasi Cocoa butter


Use it in place of coconut oil. Make chocolate candies and bars. Create creamy chocolate ice cream. Sprinkle it on top of your salad. Combine it with honey for chocolate syrup. Make chocolate pudding. Massage it into your skin. And much more!

Profil Cocoa butter

Komposisi TG pada CB
TG in Cocoa Butter
Palmitat-Oleate-Stearat (POS) Stearat-Oleate-Stearat (SOS) Palmitat-Oleate-Palmitat (POP) Palmitic Acid Stearic Acid Oleic Acid Linoleic Acid Other 24.4% 33.6% 37.0% 3.4% 1.6% 33.1% 24.7% 18.9%

FA in Cocoa Butter

SA/PA = 1.3/1

Komposisi FA pada CPO


Linoleic Acid (18:2) Myristic Acid (14:0) Oleic Acid (18:1) Palmitic Acid (18:0) Stearic Acid (18:0) 9.52% 0.99% 48.1% 40.8% 0.57%

CPO CBE
Palm Olein Similar to Cocoa Butter 48.1 % oleic acid (62% 2-position) Low cost fat Raw material for CBE 1,3 lipase specific, energy consumption, product control Problems: high cost lipase

Strategy: Lipase Immobilization


Technology: Immobilized Lipase Membrane microreactor Outcome High productivity Increase Stability

PROGRAM INSENTIF PENINGKATAN KEMAMPUAN PENELITI & PEREKAYASA KRT TAHUN 2011 WBS TEKNIK IMMOBILISASI ENZIM UNTUK PENINGKATAN STABILITAS LIPASE DAN APLIKASINYA PADA INDUSTRI PANGAN BERBASIS CPO Group Leader

WBS

Dr. Achmadin Luthfi Machsun

Program Manager
BE

WP 1 Pembuatan Lipase Amobil


Dr. Dadang Suhendar (L1) Noor Faridatun Hasanah (ES1.1)

WP 2 Pengujian Mikroreaktor
Dr. Hariyanto (L2) Raden Ibrahim (2.1)

WP 2 Karakterisasi CBE
Dr. Budiasih Wahyuntari (L3) Arie Bachtiar (3.1)

Schedule
Uraian kegiatan Persiapan Immobilisasi Lipase pada Membran Pengujian aktivitas dan stabilitas Pembuatan Cocoa Butter Equivalent Evaluasi produktivitas mikroreaktor Analisis tekno-ekonomi Koordinasi dan Pelaporan 1 2 3 4 Waktu (bulan) 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7

No

Immobilization of Lipase
New membrane Flushing with water

Clean membrane Enzyme adsorption Enzyme filtration Rinsing with buffer Active Membrane: Microreactor

SEM of Microreactor

Activity and Stability


100 80
Conversion (%)

1.50 Amount of lipase : 0.814 g/m2 1.20 0.90 0.60 0.30 0 3 6 Running time (days) 9 12

60 40 20 0

Conversion

Flux

Permete Flux (L/m2.h)

Production of CBE
CPO (Palm Olein) Stearic Acid N-Hexane Lipase

45 o C 2 days

Separation 1

Lipase

CBE

Separation 2

Residue

Karakteristik Produk

Parameter Titil Leleh Warna

37 oC Putih - Kuning

Conclussion
Immobilisasi Enzim Distribusi merata Stabilitas Lipase Lebih dari 10 hari Aplikasi pada produksi CBE

Terimakasih

CPO milling process

Scope of Work
Characterization of CPO Modification of CPO by Interesterification
Effect of Acyl donor Effect of Temperature Effect of Time Effect of Water Effect of Enzyme Amount Effect of Substrate Ratio

Isolation of CBE by TLC Analysis by GC-MS