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Chapter 11

The Prokaryotes: Domains Bacteria and Archaea

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Lectures prepared by Christine L. Case

Q&A
 Bacteria are singlecelled organisms that must absorb their nutrients by simple diffusion. The dimensions of T. namibiensis are several hundred times larger than most bacteria, much too large for simple diffusion to operate. How does the bacterium solve this problem?

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The Prokaryotes

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Domain Bacteria
 Proteobacteria
 From the mythical Greek god Proteus, who could assume many shapes  Gram-negative  Chemoheterotrophic

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The Alphaproteobacteria
Learning Objective 11-1 Differentiate the alphaproteobacteria described in this chapter by drawing a dichotomous key.

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The Alphaproteobacteria
 Pelagibacter ubique
    Discovered by FISH technique 20% of prokaryotes in oceans 0.5% of all prokaryotes 1354 genes

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The Alphaproteobacteria
 Human pathogens
 Bartonella  B. henselae: Cat-scratch disease  Brucella: Brucellosis  Ehrlichia: Tickborne

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The Alphaproteobacteria
 Obligate intracellular parasites
 Ehrlichia: Tickborne, ehrlichiosis  Rickettsia: Arthropod-borne, spotted fevers  R. prowazekii: Epidemic typhus  R. typhi: Endemic murine typhus  R. rickettsii: Rocky Mountain spotted fever

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Rickettsias

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Figure 11.1

The Alphaproteobacteria
 Wolbachia: Live in insects and other animals

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Applications p. 307

Wolbachia

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Applications p. 307

The Alphaproteobacteria
 Have prosthecae
 Caulobacter: Stalked bacteria found in lakes

 Hyphomicrobium: Budding bacteria found in lakes

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Figures 11.2b, 11.3

The Alphaproteobacteria
 Plant pathogen
 Agrobacterium: Insert a plasmid into plant cells, inducing a tumor

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Figure 9.19

The Alphaproteobacteria
 Chemoautotrophic
 Oxidize nitrogen for energy  Fix CO2  Nitrobacter: NH3 p NO2  Nitrosomonas: NO2 p NO3

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The Alphaproteobacteria
 Nitrogen-fixing bacteria
 Azospirillum  Grow in soil, using nutrients excreted by plants  Fix nitrogen  Rhizobium  Fix nitrogen in the roots of plants

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Figure 27.5

The Alphaproteobacteria
 Produce acetic acid from ethanol
 Acetobacter  Gluconobacter

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Check Your Understanding  Make a dichotomous key to distinguish the orders of alphaproteobacteria described in this chapter. 11-1

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The Betaproteobacteria
Learning Objective 11-2 Differentiate the betaproteobacteria described in this chapter by drawing a dichotomous key.

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The Betaproteobacteria
 Thiobacillus
 Chemoautotrophic; oxidize sulfur: H2S p SO42

 Sphaerotilus
 Chemoheterotophic; form sheaths

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Figure 11.5

The Betaproteobacteria
 Neisseria
 Chemoheterotrophic; cocci  N. meningitidis  N. gonorrhoeae

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Figure 11.6

The Betaproteobacteria
 Spirillum
 Chemoheterotrophic; helical

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Figure 11.4

The Betaproteobacteria
 Bordetella
 Chemoheterotrophic; rods  B. pertussis

 Burkholderia
 Nosocomial infections

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Figure 24.7

The Betaproteobacteria
 Zoogloea
 Slimy masses in aerobic sewage-treatment processes

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Figure 27.20

Check Your Understanding  Make a dichotomous key to distinguish the orders of betaproteobacteria described in this chapter. 11-2

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The Gammaproteobacteria
Learning Objective 11-3 Differentiate the gammaproteobacteria described in this chapter by drawing a dichotomous key.

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The Gammaproteobacteria
 Pseudomonadales
 Pseudomonas  Opportunistic pathogens  Metabolically diverse  Polar flagella

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Figure 11.7

The Gammaproteobacteria
 Pseudomonadales
 Moraxella  Conjunctivitis  Azotobacter and Azomonas  Nitrogen fixing

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The Gammaproteobacteria
 Legionellales
 Legionella  Found in streams, warm-water pipes, cooling towers  L. pneumophilia  Coxiella  Q fever transmitted via aerosols or milk

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Figure 24.14b

The Gammaproteobacteria
 Vibrionales
 Found in coastal water  Vibrio cholerae causes cholera  V. parahaemolyticus causes gastroenteritis

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Figure 11.8

The Gammaproteobacteria
 Enterobacteriales (enterics)
 Peritrichous flagella; facultatively anaerobic

        

Enterobacter Erwinia Escherichia Klebsiella Proteus Salmonella Serratia Shigella Yersinia

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The Gammaproteobacteria

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Figure 11.9a

The Gammaproteobacteria

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Figure 11.9b

The Gammaproteobacteria
 Pasteurellales
 Pasteurella  Cause pneumonia and septicemia  Haemophilus  Require X (heme) and V (NAD+, NADP+) factors

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The Gammaproteobacteria
 Beggiatoa
 Chemoautotrophic; oxidize H2S to S0 for energy

 Francisella
 Chemoheterotrophic; tularemia

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Check Your Understanding  Make a dichotomous key to distinguish the orders of gammaproteobacteria described in this chapter. 11-3

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The Deltaproteobacteria
Learning Objective 11-4 Differentiate the deltaproteobacteria described in this chapter by drawing a dichotomous key.

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The Deltaproteobacteria
 Bdellovibrio
 Prey on other bacteria

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Figure 11.10

The Deltaproteobacteria

 Desulfovibrionales
 Use S instead of O2 as final electron acceptor

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The Deltaproteobacteria
 Myxococcales
 Gliding  Cells aggregate to form myxospores

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Figure 11.11

Check Your Understanding  Make a dichotomous key to distinguish the deltaproteobacteria described in this chapter. 11-4

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The Epsilonproteobacteria
Learning Objective 11-5 Differentiate the epsilonproteobacteria described in this chapter by drawing a dichotomous key.

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The Epsilonproteobacteria
 Campylobacter
 One polar flagellum  Gastroenteritis

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The Epsilonproteobacteria
 Helicobacter
 Multiple flagella  Peptic ulcers  Stomach cancer

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Figure 11.12

Check Your Understanding  Make a dichotomous key to distinguish the epsilonproteobacteria described in this chapter. 11-5

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Nonproteobacteria Gram-Negative Bacteria


Learning Objectives 11-6 Differentiate the groups of nonproteobacteria gram-negative bacteria described in this chapter by drawing a dichotomous key. 11-7 Compare and contrast purple and green photosynthetic bacteria with the cyanobacteria.

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Phototrophic
 Oxygenic photosynthesis  Anoxygenic photosynthesis
light

2H2O + CO2

(CH2O) + H2O + O2

2H2S + CO2

light

(CH2O) + H2O + 2S0

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Oxygenic Photosynthetic Bacteria


 Cyanobacteria
 Gliding motility  Fix nitrogen

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Cyanobacteria

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Figure 11.13

Anoxygenic Photosynthetic Bacteria


    Purple sulfur Purple nonsulfur Green sulfur Green nonsulfur

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Purple Sulfur Bacteria

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Figure 11.14

Check Your Understanding  Make a dichotomous key to distinguish the gramnegative nonproteobacteria described in this chapter. 11-6  Both the purple and green photosynthetic bacteria and the photosynthetic cyanobacteria use plantlike photosynthesis to make carbohydrates. In what way does the photosynthesis carried out by these two groups differ from plant photosynthesis? 11-7
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The Gram-Positive Bacteria


Learning Objectives 11-8 Differentiate the genera of firmicutes described in this chapter by drawing a dichotomous key. 11-9 Differentiate the actinobacteria described in this chapter by drawing a dichotomous key.

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Firmicutes
 Low G + C  Gram-positive

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Clostridiales
 Clostridium
 Endospore-producing  Obligate anaerobes

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Figure 11.15

Clostridiales
 Epulopiscium

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Figure 11.16

Bacillales
 Bacillus
 Endosporeproducing rods

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Figure 11.17b

Bacillales
 Staphylococcus
 Cocci

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Figure 11.18

Lactobacillales
 Generally aerotolerant anaerobes; lack an electrontransport chain
    Lactobacillus Streptococcus Enterococcus Listeria

[Insert Figure 11.19]

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Figure 11.19

Mycoplasmatales
 Wall-less; pleomorphic  0.1 - 0.24 m  M. pneumoniae

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Figure 11.20a, b

Check Your Understanding  Make a dichotomous key to distinguish the low G + C gram-positive bacteria described in this chapter. 11-8

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Actinobacteria
 High G + C  Gram-positive

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Actinobacteria
        Actinomyces Corynebacterium Frankia Gardnerella Mycobacterium Nocardia Propionibacterium Streptomyces

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Streptomyces

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Figure 11.21

Streptomyces

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Figure 11.21

Actinomyces

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Figure 11.22

Check Your Understanding  Make a dichotomous key to distinguish the high G + C gram-positive bacteria described in this chapter. 11-9

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Nonproteobacteria Gram-Negatives
Learning Objective 11-10 Differentiate among Planctomycetes, chlamydias, spirochetes, Bacteroidetes, Cytophaga, and Fusobacteria by drawing a dichotomous key.

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Planctomycetes
 Gemmata obscuriglobus
 Double internal membrane around DNA

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Figure 11.23

Chlamydias
 Chlamydia trachomatis
 Trachoma  STI, urethritis

 Chlamydophila pneumoniae  Chlamydophila psittaci


 Psittacosis

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Figure 11.24b

Life Cycle of the Chlamydias

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Figure 11.24a

Spirochetes
 Borrelia  Leptospira  Treponema

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Figure 11.25

Bacteroidetes
 Anaerobic
 Bacteroides are found in the mouth and large intestine  Cytophaga: Cellulose-degrading in soil

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Fusobacteria
 Fusobacterium
 Are found in the mouth  May be involved in dental diseases

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Figure 11.26

Check Your Understanding  Make a dichotomous key to distinguish Planctomycetes, chlamydias, spirochetes, Bacteroidetes, Cytophaga, and Fusobacteria. 11-10

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Domain Archaea
Learning Objective 11-11 Name a habitat for each group of archaea.

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Domain Archaea
Extremophiles  Hyperthermophiles
 Pyrodictium  Sulfolobus

 Methanogens
 Methanobacterium

 Extreme halophiles
 Halobacterium

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Archaea

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Figure 11.27

Check Your Understanding  What kind of archaea would populate solar evaporating ponds? 11-11

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Microbial Diversity
Learning Objective 11-12 List two factors that contribute to the limits of our knowledge of microbial diversity.

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Microbial Diversity
 Bacteria size range
 Thiomargarita (750 m)  Nanobacteria (0.02 m) in rocks

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Figure 11.28

Microbial Diversity
 PCR indicates up to 10,000 bacteria per gram of soil.  Many bacteria have not been identified because they
 Haven't been cultured  Need special nutrients  Are a part of complex food chains requiring the products of other bacteria  Need to be cultured to understand their metabolism and ecological role

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Q&A
 Bacteria are singlecelled organisms that must absorb their nutrients by simple diffusion. The dimensions of T. namibiensis are several hundred times larger than most bacteria, much too large for simple diffusion to operate. How does the bacterium solve this problem?
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Check Your Understanding  How can you detect the presence of a bacterium that cannot be cultured? 11-12

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