Protein Synthesis
Gene
DNA RNA
coding
template
Protein
Gene Therapy
Also known as somatic gene therapy Using genes as medicine
In vivo technique
Requires no surgery Therapeutic DNA is injected into body cells
- adenovirus
2. Gene Silencing
Class of undifferentiated cells that are able to differentiate into specialized cell types. Two main sources :
- Embryonic stem cells - Adult stem cells
Capable of dividing and renewing themselves for long period Unspecialized Can give rise to specialized cell types
Adult stem cells are undifferentiated cells found throughout the body that divide to replenish dying cells and regenerate damaged tissues.
Human embryos reach the blastocyst stage 4-5 days post fertilization.
Cells divide and spread over the surface of the dish. Inner surface of the culture dish is typically coated with mouse embryonic skin cells that have been treated so they will not divide.
The mouse cells in the bottom of the culture dish provide the cells sticky surface for attachment. Removed gently and plated into several fresh culture dishes. This process is repeated.
differentiating (pluripotent)
Given their unique regenerative abilities, stem cells offer new potentials for treating diseases such as diabetes, and heart disease.