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Chapter 8

What is aseptic technique?


Prevents contamination i.e. introduction of environmental organisms.
Examples? Clean benches with vesphene Keep bookbags, etc off benches Wash hands after lab Pages 64 aseptic technique

Page 64

Chapter 9

Bacteria always exist in nature in mixtures. In order to study a particular organism, it is necessary to isolate it.

PURE CULTURE
Culture consisting of one kind of microorganism.

COLONY
Population consisting of one kind of micro-organism. STREAK PLATE Dilution technique used to isolate an organism.

Common streak plate mistakes:


Taking lid off completely or placing on bench top Not flaming between quadrants Going back into previous quadrant too many times Labeling lid

Not letting loop cool sufficiently


Streaking bacteria onto lid Writing on agar