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  • Enzamatic Functionalization Final

    Diunggah oleh

    mlg54
    23 tayangan16 halaman
    Immobilized molecule is one whose movement in space has been restricted either completely or to a small limited region by attachment to a solid structure. The term immobilization refers to the act of the limiting movement or making incapable of movement i.e., retard the movement.

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    Immobilized molecule is one whose movement in space has been restricted either completely or to a small limited region by attachment to a solid structure. The term immobilization refers to the act of the limiting movement or making incapable of movement i.e., retard the movement.

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    Attribution Non-Commercial (BY-NC)
    Unduh sebagai PPT, PDF, TXT atau baca online dari Scribd
    Tandai sebagai konten tidak pantas
    23 tayangan16 halaman

    Enzamatic Functionalization Final

    Diunggah oleh

    mlg54
    Immobilized molecule is one whose movement in space has been restricted either completely or to a small limited region by attachment to a solid structure. The term immobilization refers to the act of the limiting movement or making incapable of movement i.e., retard the movement.

    Hak Cipta:

    Attribution Non-Commercial (BY-NC)
    Unduh sebagai PPT, PDF, TXT atau baca online dari Scribd
    Tandai sebagai konten tidak pantas
    dari 16

    To produce Techno Green Textiles 1 of 15

    iit
    Delhi

    Textile

    Modified Substrate

    Enzyme

    Functiona lization

    Smart Textile
    2 of 15

    iit
    Delhi

    Textile

    Enzyme

    Immobiliz Immobiliz ation of ation of Enzyme Enzyme

    Smart Textile
    3 of 15

    iit
    Delhi

    An immobilized molecule is one whose movement in space has been restricted either completely or to a small limited region by attachment to a solid structure. In general

    the term immobilization refers to the act of


    the limiting movement or making incapable of movement i.e., retard the movement

    4 of 15

    iit
    Delhi

    Adsorption
    Enzyme

    Textile Substrate
    ADVANTAGES No chemical change High retention of activity Reversibility DISADVANTAGES Easy desorption Non specific bonding Overloading of support

    5 of 15

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    Delhi

    Covalent Bonding
    Enzyme

    Textile Substrate
    ADVANTAGES No desorption Better thermal stability DISADVANTAGES High cost Loss of enzyme activity

    6 of 15

    iit
    Delhi

    Photochemical Immobilization
    Enzyme Cross linker

    Textile Substrate
    Cross linked Enzyme

    UV Cured Textile Substrate


    7 of 15

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    Delhi

    Entrapment in Polymer Gel


    Polymer Gel Textile Substrate
    ADVANTAGES DISADVANTAGES Enzyme can be Encapsulated Diffusion and Possibility of co-immobilization accessibility Journal of Biomaterials and Nanobiotechnology, 2010, 1, 61-77 8 of 15

    Enzyme

    iit
    Delhi

    Immobilized Enzymes on Nanofibres


    Nano Fibres of Polystyrene attached with Nitrophenyl ending groups
    - NH

    -NH2 + Enzyme

    Enzyme

    Biotechnol. Prog. 2002, 18, 1027-1032

    9 of 15

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    Delhi

    Layer by Layer Coating

    10 of 15

    iit
    Delhi

    COO - NH - Enzyme
    OR

    - Alk. Pactinase
    - Amylase -Laccase
    30 - 40 mg/100g o Escherichia coli o Staphylococcus aureus o Candida albicanes, fungi o Asparigillus niger, fungi

    Modified Cotton

    Cu Enzyme complex

    1. BTCA + CMC # (COOH ) 2. PEI + CuCl2 # (NH2)

    Journal of Applied Polymer Science, Vol. 104, 17541761 (2007) 11 of 15

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    Delhi

    Bacteriostatic to Staphylococcus aureus

    Bioprocess Biosyst Eng (2009) 32:633639 12 of 15

    NH2 Glutaraldehyde 250C for 6 h

    NH Lysozyme (1, 4-b-Nacetylmuramidase 40C, pH 7.0, 6 h

    Enzyme

    NH NH

    iit
    Delhi

    Wool fabrics stained with Coomassie Brilliant Blue G-250


    (a) Untreated wool fabric, (b) Hydrogen Peroxide pretreated wool fabric, (c) Glutaraldehydeactivated wool fabric and (d) Wool fabric immobilizing lysozyme

    13 of 15

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    Delhi

    Decontamination of Organophosphorous Pesticides 1. Organophosphorous Hydrolase (OPH) hydrolysis Methyl Parathion and Nerve Agents 2. OHP and other enzymes denature rapidly under these conditions 3. Immobilization of OPH and other enzymes on solid supports via covalent or physical bonding can extend bioactivity of these enzymes 4. Polyelectrolyte multi-layering (PEMs) formed via Layerby-Layer adsorption are useful in encapsulation of enzymes.

    14 of 15

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    Delhi

    Layer by Layer Deposition of Enzymes


    OPH Enzyme BPEI - Branched Polyethylenimmine BTP bis-trispropane buffer to OPH solution PSS Polystyrene Sulphonate
    Adv. Mater. 2004,16,2112

    15 of 15

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