DAFTAR ISI

DAFTAR ISI................................................................................................... iv
DAFTAR GAMBAR ...................................................................................... vii
BAB I PENDAHULUAN ............................................................................... 1
1.1 Latar Belakang ................................................................................. 1
1.2 Rumusan Masalah ............................................................................ 4
1.3 Maksud dan Tujuan .......................................................................... 4
1.3.1 Maksud Penelitian ......................................................................... 4
1.3.2 Tujuan Penelitian ........................................................................... 4
1.4 Manfaat Penelitian ............................................................................ 5
BAB II TINJAUAN PUSTAKA ................................................................... 6
2.1 Mikroalga ......................................................................................... 6
2.2 Faktor faktor yan Mempengaruhi Pertumbuhan Mikroalga .......... 9
2.2.1 Unsur Hara .................................................................................... 10
2.2.2 Suhu ............................................................................................... 10
2.2.3 Cahaya ........................................................................................... 11
2.2.4 Salinitas ......................................................................................... 12

2.2.5 Derajat Keasaman .......................................................................... 12

2.3 Tinjauan Umum Nitzchia sp ............................................................. 12
2.4 Polisakarida dan Ekstrak Intraseluler ............................................... 14
2.5 Antioksidan ...................................................................................... 16
2.6 Antimikroba ...................................................................................... 17
2.7 Fungi ................................................................................................. 19
2.7.1 Candida Albicans .......................................................................... 20
2.7.2 Malassezia furfur ........................................................................... 21
2.8 Komponen Aktif ............................................................................... 22
2.8.1 Alkaloid ......................................................................................... 22
2.8.2 Flavonoid ....................................................................................... 22
2.8.3 Steroid ........................................................................................... 23
2.8.4 Saponin .......................................................................................... 23
2.8.5 Fenol Hidrokuinon ........................................................................ 24
BAB III METODOLOGI PENELITIAN .................................................... 25
3.1 Bahan Penelitian ............................................................................... 25
3.2 Alat Penelitian .................................................................................. 25

3.3 Waktu dan Tempat Penelitian .......................................................... 26

3.4 Prosedur Penelitian ........................................................................... 26
3.4.1 Preparasi Sampel ........................................................................... 26
3.4.2 Perhitungan Jumlah Sel Nitzschia sp ............................................. 26
3.4.3 Pemisah Ekstrak Intraseluler ......................................................... 27
3.4.4 Ekstraksi Polisakarida ................................................................... 27
3.4.5 Analisis Komposisi Biokimia Biomassa Nitzschia sp ................... 28
3.4.5.1 Analisis Kadar Air ...................................................................... 28
3.4.5.2 Analisis Kadar Abu .................................................................... 28
3.4.5.3 Analisis Kadar Protein ................................................................ 28
3.4.5.4 Analisis Kadar Lemak ................................................................ 30
3.4.6 Uji Fitokimia ................................................................................. 30
3.4.6.1 Uji Alkaloid ................................................................................ 31
3.4.6.2 Uji Steroid .................................................................................. 31
3.4.6.3 Uji Flavonoid .............................................................................. 32
3.4.6.4 Uji Saponin ................................................................................. 32
3.4.6.5 Uji Hidrokuinon ......................................................................... 32

3.4.7 Penyiapan Fungi Uji ...................................................................... 32

3.4.7.1 Peremajaan Fungi Uji ................................................................. 33
3.4.7.2 Pembuatan suspensi Fungi Uji ................................................... 33
3.4.8 Uji Aktivitas Antijamur ................................................................. 33
3.4.9 Uji Aktivitas Antioksidan .............................................................. 33
3.4.9.1 Uji Kualitatif Aktivitas Antioksidan .......................................... 33
3.4.9.2 Uji Kuantitatif Aktivitas Antioksidan ........................................ 34
DAFTAR PUSTAKA ..................................................................................... 35
LAMPIRAN ................................................................................................... 43

DAFTAR GAMBAR
Gambar.1 Morfologi Nitzschia sp .................................................................... 13

DAFTAR LAMPIRAN
1. Kultur Nitzschia sp ............................................................................. 43
2. Bagan Isolasi Senyawa Polisakarida dan Ekstrak Intraseluler ............ 44
3. Bagan Analisis Komposisi Biokimia Biomassa Nitzschia sp .............. 45
4. Bagan Uji Fitokimia ............................................................................ 47
5. Bagan Uji Antijamur ............................................................................ 50
6. Bagan Uji Antioksidan ........................................................................ 51