1LKNlK KLL1Lk KLL 1LKNlK KLL1Lk KLL

Oloo.
Adi kurniowon 81!007011
Adindo kocbim N.I 81!008151
Moboroni Nursyomsu 81!009005
Devi OIivio MuIiowo|i 81!009088
Voocrct lcsltosorl ct o|. (2008}, to|ol| |cltcr ortoo
|oll ullo|c|oo borosol uorl otoooo |orlooo yoo
ultcbco|oo ul otos scbstrot, sooloo ortcbcooo
oooyo torbotos ouo sol-sol yoo borlrosl uorl
otoooo |orlooo torsobct.
oboroo o|sloo yoo ulcoo|oo uolo |cltcr ootoro
lolo sol, |orlooo, oroo, .c|| ||nc. Dowoso lol booyo|
ooolltloo yoo oo|cltcr stcm .c|| cotc| oouoot|oo
sol-sol yoo uoot uloroo|oo ulIorooslosloyo.
kloolo
orco|oo tlouo|oo cotc| obcot
roll|o,ucll|ot uorl soscotc.
Dl|oool 3 |ools |loolo blolol
V kloolo tlo|ot DNA
V kloolo cotc| coyo torol
V kloolo cotc| korouc|sl
l. kloolo tlo|ot DNA
P kloolo ullo|c|oo toroouo cotoloo DNA cotc|
oouoot|oo cotoloo DNA yoo luootl|, yoo
|oculoo oocoo|oo loslu bo|torl
oooosll|oo olo|cl uoooo slIot oootl| yoo
soo cotc| |oootlooo o.l. obcotoo
ooo|loool ootlbouy cotc| |oorlcoo ulooostl|,
obcotoo vo|slo usb.
2. kloolo cotc| tc|coo torol ooobotoo
(Joorooctlc Cloolo}
kloolo ultc|c|oo cotc| oooosll|oo sto coll (sol
botoo}.
8to coll lol uloooo uorl |loolo yoo
oooosll|oo obrlo oocslo, ooco tluo|
ul|oboo|oo oo|oul oolc| borc.
3. kloolo cotc| korouc|sl
(koroucctlvo Cloolo}
P cotc| oouoot|oo |otcrcooo, otoc ooouo|oo
ortoboooo |cloo oroolso.
P Doooo ultoc|oooyo to|oolol |loolo cotc|
|oootlooo rorouc|sl, borortl obc|o olcoo cotc|
oooboo|oo loucstrl rorouc|sl oocslo, oowoo,
occo tcbcooo uol |oootlooo blsols.
P 8ol tolcr otoo yoo ulbcoo lotl soloyo, |o uolooyo
|oculoo ulscotl||oo lotl sol sootl|, sooloo sol yoo
|oculoo torbootc| ulcoyo|oo cotc| tcbco |oboo
oooosll|oo oolc| borc. uol lol sorco uoooo
rorouc|sl voototlI, tooo ololcl rosos obcoooo sol
tolcr oloo boolo lo|l-lo|l
$tcm .c|| ($c| lun.o)
$tcm .c|| sol-sol yoo bolc borulIorooslosl, uoot borrollIorosl,
uoo uoot ullouc|sl cotc| borulIorooslosl oo|oul borbool tlo sol
uoooo Icosl |ocscs.
Voocrct 1cscI, (2009} slIot |oos.
l. DlIIorooslosl (|IIccnt|otc} |oocoo cotc| bor|oboo oo|oul
sol lolo,|oos (soslIl|} lsoloyo sol soroI, sol otot |ootco, sol otot
roo|o, sol oo|roos uoo lolo-lolo.
2. koooorosl ($c|I cqcncotc,sc|I cncw} |oocoo cotc|
oorbooorcl otoc oroooorosl ulrloyo sooulrl, oc obcot
sollooo sol yoo orsls soo uoooo ulrloyo ololcl oboloooo sol
Voocrct 1oos (200l}, |ools sto sol uolo tcbco oocslo uoo
oowoo.
o. 8ol sto obrlool| (|bryoolc 8to Colls}
b. Noo-|bryoolc 8to Colls (8ootlc,Auclt 8to Colls}.
o. 8eI s|em embrionik (Lmbryonic 8|em CeIIs)
sol yoo ulobll uorl |nnc .c|| moss -
scotc |ccloo sol yoo torloto| ul sotc
slsl b|osto.st yoo borccr 5 oorl uoo
torulrl uorl l00 sol.
uoot bor|oboo blo| socoro torcs
ooorcs uolo oulo |cltcr otlol
uoo ouo |oouooo tortootc uoot
uloroo|oo cotc| borulIorooslosl oo|oul
borbool sol yoo torulIorooslosl
soortl sol |ootco, sol |cllt, oocroo,
oootoslt uoo sobooloyo.
Doot borslIot tcorlool|
ortloyo sotlo |ootoloosl
uoooo sol yoo to|
borulIIorooslosl uoot
oolbcl|oo |oo|or.
8ololc borslIot olloool|
sooloo borotoosl
oolbcl|oo tor|ouloyo
ro|o|sl lcoltos
8ocoro otl| oslo
|ootrovorslol.
Vcuoo uluoot|oo uorl |llol|
Iortlllto
orslIot lcrlotoot ocoyol
|oocoo cotc| borulIIorooslosl
oo|oul borbool oco sol yoo
orco|oo tcrcooo uorl |o 3 lols
orlool (o|touor, osouor uoo
oououor}, totol tluo| uoot
obootc| solcbco obrlo.
lortol uoot borccr oo|oo
sooloo uoot oorbooyo| ulrl
rotcsoo |oll ouo oulo |cltcr.
koo|sl ooolo|oo roouoo
koloblooo
ko|croooo
b. Non-Lmbryonic 8|em CeIIs
(8omo|ic[AduI| 8|em CeIIs).
toruoot ul soco oroo tcbco, torctoo
ul uolo scsc tcloo
|cosl. olo|c|oo roooorosl cotc|
oorbol|l |orlooo yoo ooolol
|orcso|oo.
Jcbco |lto ooolol oorcso|oo oloo
borbool Io|tor uoo ooo|lbot|oo
oo|rosls (|ootloo |orlooo uoo sol} o|oo
ulborslo|oo oloo sol o|roIo yoo
borouor uolo uoroo.
8cbor. Iotcs (Icto| stcm .c||s), scsc
tcloo (bonc moow stcm .c||s), uoroo
orlIor otoc toll csot (umb|||.o| .oJ
b|ooJ stcm .c||s, uC8).
1cloooyo soul|lt uoo
sooot |oroo ultoc|oo
ouo |orlooo otcro
scllt oouoot|oo ouclt
sto colls uolo |cloo
booyo|.
Voso olucoyo tluo|
soloo obryoolc sto
colls
orslIot cltlotoot
sooloo ulIIorooslosl tluo|
solcos obryoolc sto colls
yoo borslIot lcrlotoot.
Doot uloroloo uorl sol osloo
sooulrl sooloo ooolouorl
tor|ouloyo ooolo|oo lco.
8cuoo torsoslollsosl sooloo louc|sl
oo|oul loblo souoroooo
kcroo otoc tluo| oolbcl|oo
roblo otl|o.
koloblooo
ko|croooo
81LM CLll8 DAk| UM8|l|CAl COkD
8lOOD
loocoooo osoocoyol sto coll uorl uoroo toll csot oo|oul roIoroosl
soboloo ooolltl |orooo slIot lostlsltosoyo yoo tlol uoo
lcoooolsltosoyo roouoo.
Doooo orlo|coo toot, osoocoyol sto coll uoot bor|oboo oo|oul
borbool sol uoot ulo|ol sobool scbor trooslootosl sol cotc| torol
borbosls sol.
oocoooo osoocoyol sto coll sobool torol rctlo ouo oocslo
oo|cltcr osoocoyol sto coll uolo oulc ooo-Iroo cotc| oocooo
troosIor otooo oowoo |o oocslo.
Vosoocoyol sto coll yoo ultcbco|oo ul borbool loborotorlc uoooo
borbool to|ol| olll|l 2 |osoooo yoltc. tcbco ul |cltcr sobool sol
yoo olo|ot uoooo loo oluc tortootc, uoo olll|l |oocoo cotc|
borulIorooslosl oo|oul ostooblost, cooouroblost, uoo oulocyto uolo
rosoo toroouo stlcll yoo toot.
8ocoro olstolols, osoocoyol sto coll olll|l bootc| IcslIor, Ilbroblost-
ll|o, uoo ouo Ioso ortcbcooo lo vltro owol obootc| |olool.
TFKtlK-TFKtlK SPFSlAl Kl0tlt6
1. Kl0tlt6
1. PFt6FtCFRAt
2. TFKtlK SuSPFtSl A6AR
3. TFKtlK PFlAPlSAt
2. KulTuR 0R6At/JAR
3. KulTuR TRAKHFA FH AYAH
klooo oclosl sol yoo borosol uorl sotc sol
tcool lowot ltosls
8trolo |looo scotc oclosl sol yoo borosol
(ultcrco|oo} uorl scotc |looo 8 obowo clrl2 8
slIot2 y sololc ouo sooo|oo |cltlvosl loo|ctoo
Jo|ol| |loolo ocoyol booyo| oll|osl, o.l .
looostobllsoo 8 ocroloo llotosoo sol
lsolosl vorloo2 sol
|volcosl orlo|coo2 oorlootol 8 |ooslstoosl oootl| sol
8tcul ulIorooslosl Icosl sol
Jo|ol| cotc| lsolosl 8 oocbcooo sol tcool .
Voocoo|oo lo |ollor
Jo|ol| oooocoroo
Jo|ol| scsoosl oor
loocoooo ol Ilbrlo
Dolo |loolo Io|tor2 ttt ors ulorootl|oo,ol , |ouor o|sloo ors ul|ootrol,
orlc oocoooo sorc, boooo2 to|sl| ors ulolouorl
koosltos ortcbcooo |cltcr ulc|cr u oIlsloosl lotlo. kcltcr u oIlsloosl
lotlo roouoo ( l0%} loblo scllt c, ul |looo 8 blosooyo oorlc|oo
|ooulsl yoo loblo ostl
kt| borotobollso sol2 oooosll|oo rouc|2 y sooot ootlo c,
ortcbcooo
Dl scsoosl u |oouotoo roouoo, rouc|2 lol toroocor|oo ul oulc 8
sooloo-oloo tu| oocc|cl b sol so ors ultoboo|oo rouc|2 soortl
lrcvot, oso2 oloo oooosooslol 8 CO2
koborooslloo |loolo torootco u olllooo y loyo| 8 oolotorosloo
sol, oulc, to|ol| lsolosl, |ooulsl lo|cbosl, 8 ouol ro|ovorl
KONNG DG METODE PENGENCERAN
BuIun
SuspensI seI HEp-z (1ooo seIJmI)
MedIum perLumbuIun, 1% IeLuI bovIne dIm medIum eugIe
empeng mIkrokuILur, q6 weII
Curu kerju
SIupkun pengencerun IIpuL z suspensI seI dIm medIum perLumbuIun (1:z - 1;1:1z), gunukn voI. z mI
Dg pIpeL kupIIer sLerII LumbuIkun 1 LLs berbuguI pengencerun (Ldk dIencerkun, 1:1z) pd seLIup 8 weII
TumbuIkun z LLs medIum perLumbuIun pd seLIup weII Iempeng mIkrokuILur Lsb.
LuLup Iempeng & InkubusIkun pd ;C dIm uLmosIer Iembub yg berIsI % COz dIm uduru
SLII q jum LIup weII Iempeng mIkrokuILur dIujI dg mIkroskop & LunduI weII yg Iny berIsI 1 seI LungguI
Teruskun InkubuI Iempeng sumpuI monoIuyer LerbenLuk dIm weII yg berIsI 1 seI LungguI
GunLI medIum pd InLervuI q IurIJbIIu perIu & rekover seIz yg dIkIone dg prosedur LrIpsInusI ruLIn
kuLJuk AGAk
KulTuR A6AR
Aor ooyoulo|oo |ooulsl torsoslollsosl y oocotco|oo u
tlo ooolltloo ttt.
Aor ut ulcoo|oo sb scotc scbstrot c, ortcbcooo sol 8 sb scotc
scort ouot c, |cltcr oroo oor ulcocr u oulc
octrloo 8 ulor|oros u obclco |cltcr y ullolo|oo. 8ol,|orlooo
ul|cltlvosl u orc|ooo oor
Jo|ol| cc olols|oo scotc cocroo sol y ulscsoosl|oo
u scotc |oosootrosl oor lcoo| ulotos lolsoo uosor oor y lbo ouot 8
lbo tor|oosootrosl. 8ol2 torscsoosl 8 tu| ut bororo| |ro oor sorto
tcbco sb |olool2 loulvlucol. Jo|ol| lol ut ulcoo|oo c, |loolo sol.
kcltcr oor ut ooyoulo|oo ortcbcooo solo|tlI y oocotco|oo
tlo2 sol ttt
Aor | ulcoo|oo c, olo|ollsosl loIo|sl u oooloyor y tlo ulloo|closl
u oooslo loIo|tlcs st vlrcs. 8ol oooloyor y torloIo|sl
ultcool u scotc lorctoo y ulroorosl ul oulc octrloo. Jt| csot
loIo|sl ulsobct logco.
loworoo vltol, ooctrol rou sorlo ulcoo|oo u |cltcr oor c,
oooto|oo vlobllltos sol 8 c, oooc|oo logco
loworoo vltol ut loosco ulcocr|oo u lorctoo oor otoc
u orc|ooo oor y ouot 8 ulblor|oo borulIcsl
Noctrol rou olll|l scotc oIo| Iotoulool| oyoto, |cltcr y
oocoo|oo ooctrol rou ut torllouco ur coooyo so sol
o|oo toto vloblo
loworoo lolo . |rlstol vlolot, corbol Iccoslo otoc loso
kotl|o |oosootrosl oor roouoo otoc ulorcoo|oo otoyl
solcloso, ololsoo ut uloo|ot u soot y ullolo|oo 8 sol
ulworool u |rlstol vlolot, |orbol Iccoslo otoc loso. Doooo
to|ol| lol, |cltcr ut ulworool otoc ulrooto||oo.
J|kNlk 8u8l|N8l AGAk, l|kJuVuuAN kOLONl 8|L
AuAN
8csoosl u|-2 . 8.000.000 sol,l ul oulc ortcbcooo oolo (l0% Iotol
bovloo sorc}
Voulc ortcbcooo, |oosootrosl 2x, 20% Iotol bovloo sorc ul oolo
(ulooos|oo =5C uclc}
Aoroso, l%
Noctrol rou, l . l000
Cowoo |cltcr lostl|
CAkA k|k1A
l. Colr|oo ooroso l% uoooot|oo |ootoloor u olr ooululo. llouoo|oo lorctoo
y oocolr u wotor boto =5C c, ululolo|oo 8 oooooooo
2. lroorosl loloo uosor oor
o. Cocr|oo boloo l% ooroso 8 oulc ortcbcooo oolo (|oosootrosl 2x},
|oosootrosl ooroso |u 0,5%, ortoooo|oo u scoc =5C
b. llot = l ooroso o,5 % |o ul sotlo cowoo |cltcr
c. Jctc 8 loto||oo cowoo u scotc orc|ooo sool ooroso oouot
3. lroorosl ooroso olols sol
o. Cocr boloo o|col scsoosl sol u|-2 8 ooroso 0,5%, |oosootrosl
ooroso |u 0,25% 8 |oosootrosl o|olr sol =.000.000 sol,l
b. Lols|oo ul otos lolsoo oor uosor ur sotlo cowoo u ll cocroo
ooroso-sol
c. Jctc 8 loto||oo cowoo u scotc orc|ooo sool cocroo |u
ooootol
=. lo|cbosl |cltcr u 37C ul otosIor bor|oloboboo 5% CO2 ul cuoro.
Loto||oo cowoo u oslsl oooouo |o otos sooloo oor borouo ul
uosor cowoo
5. Worool sol soscuoo lo|cbosl 2= |o
o. Colr|oo ooctrol rou l.l0 ul scotc lorctoo ooroso 0,5% c, oborl
oooocoroo o|olr l.l0.000 (0.0l%}
b. Joboo|oo 2 l lorctoo ooctrol rou ooroso u sotlo |cltcr
c. lor|oo lorctoo ooootol 8 |oboll|oo |cltcr u lo|cbotor
. kcltcr ulootl u l|ros|o sotoloo lo|cbosl soloo l = |o. Noctrol rou
borulIcsl lowot oor |o uolo sol. loworoo ut ultoc|oo ul sltoloso
sol2 oluc. 8ol2 otl tu| ooyoro oworoo.
J|kNlk l|LAll8AN, A8A lLAOu| vlku8
keoqensio :
voro oooloyor, botol 25 c
Cocroo ololsoo oor y borlsl ooctrol rou (ul|oo u =5C}
lollovlrcs, uloocor|oo ul 88 uoo|
88 uoo|
Coro |or|o
Abll oulc ortcbcooo ur |cltcr sol voro
Cccl sol oooloyor 3 u 88 uoo| vol. 5l
loo|closl|oo 0,2 l vlrcs |o ul slo sotc oooloyor. loo|closl|oo |cltcr |ootrol u 0,2 l 88
uoo|. koot|oo tctc u sotlo botol
lo|cbosl |cltcr l,5 |o u toorotcr |oor. Goyoo2|oo |cltcr sotlo l5 oolt
Joboo|oo cocroo oor olols volco 7 l u sotlo |cltcr, root|oo tctc botol
Loto||oo |cltcr u orc|oooo y booor2 uotor soloo 30 oolt
oll||oo |cltcr (oor ul otos} 8 lo|cbosl|oo u 35C soloo 3 oorl (lloucol
|cltcr ur coooyo}
ultco logco y bor|oboo ul |cltcr
Joto|oo colt2 obootc| logco (l|u} u oocoo|oo rccs borl|ct .
1cloo logco l, vol. loo|clc Io|tor oooocor = l|u,l
kol|closl colt obootc| logco (l|u}.
1cloo logco = 2l
vol. loo|clc = 0,2 l
loooocoroo vlrcs = l . l.000.000
2l l,0,2 l.000.000
= l.050.000 l|u,l
kuLJuk OkGAN DAN 1AklNGAN
kuLJuk OkGAN DAN 1AklNGAN
kcltcr sol |cltlvosl sol socoro loulvlucol
kcltcr oroo,|orlooo oolloorooo,|cltlvosl |oloo| sol y
tororoolsosl
8ol2 y tororoolsosl |osolcrcooo oroo,|orlooo, bo. 8cotc oroo,
otoc coloo oroo
|cosl oroo,|orlooo y ul|cltcr ulortoooo|oo 8 sol2 tsb uloroo|oo |o
or|oboooo oorol st y ul|col u |n v|vo, sb cootoo . ulIorooslosl otoc
roroosl
All|osl |cltcr oroo .
l. DlIorooslosl obrlool|
2. loo|o|loo Ilslolol| c, oooto|oo oIo| scbstoosl st ooroo, |orsloooo 8
vltolo
3. lortcbcooo oooslo l|roblolol| st vlrcs, bo|torl 8 l|oloso
=. 8lto|llo, octorouloroIl 8 stcul cltrostrc|tcr
Jo|ol| cotc| |cltcr oroo .
Voooot|oo otoooo |ocll |orlooo u scotc ooyo|oo y ul |oouooo
|ooto| u scotc oulc octrloo ouot,colr
kcltcr ullo|cbosl ul scotc llo|cooo y toorotcr, |oloboboo 8 |ouor
O2,CO2 ulotcr
Jo|ol| oroouo |cltcr |orlooo ulroouo scr ooco ul oulc so
orc|ooo |orlooo oouoot|oo scotc llo|cooo y colIor
lu owol |cltcr oroo, oulo octrloo ouot st |oouoloo loso 8 oor
ulcoo|oo
8oot lol, oulo stoouor |cltcr sol y ul|obloosl u boroo slsto ooyo|oo
loblo sorlo ulcoo|oo
Cootoo slst. looyo|oo . ro|lt ur |ortos ,solcloso osotot y oooco, oto |olo
rlu loo ootl|orot (stololoss}, sooo solcloso borlols |olooo, bclo Ilbrlo,
olos ooctc y ulbco|cs otoc obroo vltolloo
Voulc 8 slst. looyo|oo ullllo otos uosor tlo |orlooo 8 |oorlcoo ooolltloo
vlobllltos |cltcr ultootc|oo oloo otouo olstolols, ootoooo, oworoooo 8
ooootoo l|ros|ols
kuLJuk OkGAN JkAku|A AAV
oooo .
Ayo ccr 2-3 loc
Voulc orowotoo oolo ul 88 |orll
88 uoo|
Alr uostllosl storll
kootoloor . cowoo otrl lostl| 0
cowoo |cltcr oroo lostl| u rlu loo ootl|orot
tobco |cltcr bortctc
Coro |or|o .
uowoo ulbcoco u os CO2 Jrocooo ulobll 8 toot|oo u 88 uoo|
Jrocooo ulotoo u s|olol,cotlo |u cloclo tlls 8 cccl
Cloclo trocooo ulborslo|oo ul 88 uoo|
Cloclo trocooo ul|cltcr ul sotlo obclco . cowoo otrl, tobco roo|sl, cowoo |cltcr
oroo u rlu loo ootl|orot
BuuL guruLun IuIus q-6 ureu kecII pd dusur cuwun peLrI
TempuLkun cIncIn LrucIeu pd seLIup ureu guruLun
TumbuIkun MEM secukupnyu sumpuI merendum cIncIn LrucIeu
TempuLkun penuLup pd cuwun
eLukkun kuILur pd ;C dIm uLmosIer Iembub % COz dI uduru
Cuwun peLrI
Tubung reuksI
PInduIkun poLongun cIncIn LrucIeu ke Lubung kuILur LerLuLup rupuL
BIurkun cIncIn LrucIeu menempeI dIndIng kucu 1J dusur Lubung
TumbuIkun 1 mI MEM & LuLup Lubung kuILur
eLukkun kuILur pd drum berpuLur pd ;C
Kocok Lubung kuILur seLIup zq-q8 jum uJ meIepuskun cIncIn dr kucu
GunLI medIum seLIup IurI
CAWAN kuLJuk DGN GklD LOGAV ANJlkAkAJ
l. Joot|oo cloclo trocooo u rlu loo ootl|orot
2. D Iorco storll, louoo|oo rlu loo ul toooo2 woll cowoo |cltcr
oroo
3. Joboo|oo so|ltor 0,9 l V|V |o ortoooooo woll. Jctc cowoo |cltcr
=. lo|cbosl |cltcr u 37C ul otosIor lobob 5% CO2 ul cuoro
5. Lobob|oo cowoo obsorboo ul cowoo |cltcr oroo u 5 l olr
uostllosl storll
. Aotl uloulo borrslllo bol ul cloclo trocooo c, oro|oooyo u
l|ros|o lovortou,l|ros|o ulso|tlo u coooyo ootcl. ooulo|oo
u |cltcr2 u otouo borbouo