LAPORAN AKHIR

PRAKTIKUM ANALISIS FARMASI

PERCOBAAN II

Penetapan Kadar Kloramfenikol dalam Salep dengan Metode Spektrofotometer UV

Disusun oleh :

Kelompok/ Golongan : 3/ 2B
Nama Anggota : Reza Nur Iman G1F012056
: Retno Widiastuti G1F012058
: Anita Kurnia G1F012060
: Sausa Monica G1F012062
: Nisadiyah F. Shahih G1F012064
Tanggal : 23 Oktober 2014
Asisten : Nurmaningtyas Fitri Rahmawati

LABORATORIUM KIMIA FARMASI

JURUSAN FARMASI
FAKULTAS KEDOKTERAN DAN ILMU-ILMU KESEHATAN
UNIVERSITAS JENDERAL SOEDIRMAN
2014
 Penetapan Kadar Kloramfenikol dalam Salep dengan Metode Spektrofotometer UV

A. TUJUAN

B. ALAT DAN BAHAN

C. DATA PENGAMATAN
1. Penimbangan larutan standar (weighing by difference)

Wadah + kloramfenikol standar = 241 mg

Wadah + sisa kloramfenikol standar = 191 mg

Bobot kloramfenikol standar = 50 mg

2. Pembuatan larutan induk

Bobot kloramfenikol standar = 50 mg

Volume induk = 25 ml

Kadar larutan induk = 2000 ppm

3. Pembuatan seri konsentrasi

Kadar1 (ppm) V1 (ml) Kadar2 (ppm) V2 (ml)
2000 1 80 25
80 1 8 10
80 3 24 10
2000 1 200 10
200 1 20 10
200 2 16 25
200 3 60 10
60 2 12 10
60 3 18 10
4. Pembuatan kurva baku
Kadar (ppm) Serapan (A)
8 2,543
12 2,575
16 2,568
18 2,566
20 2,69
24 2,58
5. Preparasi sampel
Kadar sampel (tertera dalam kemasan) = 2 %

Sampel 1

Penimbangan sampel (weighing by addition)

Wadah = 23800 mg
 Wadah + sampel = 24920 mg

Zat = 1120 mg

Volume etanol total = 5 x 10 ml = 50 ml

Kadar sampel dengan faktor pengenceran 25

Kadar 1 (ppm) V1 (ml) Kadar 2 (ppm) V2 (ml)

448 1 17,92 25
Serapan = 2,569 A

Sampel 2

Penimbangan sampel (weighing by addition)

Wadah = 23800 mg

Wadah + sampel = 24845 mg

Zat = 1045 mg

Volume etanol total = 5 x 10 ml = 50 ml

Kadar sampel dengan faktor pengenceran 25

Kadar 1 (ppm) V1 (ml) Kadar 2 (ppm) V2 (ml)

418 1 16,72 25
Serapan = 2,550 A

Sampel 3

Penimbangan sampel (weighing by addition)

Wadah = 23800 mg

Wadah + sampel = 24855 mg

Zat = 1055 mg

Volume etanol total = 5 x 10 ml = 50 ml

Kadar sampel dengan faktor pengenceran 25

Kadar 1 (ppm) V1 (ml) Kadar 2 (ppm) V2 (ml)

422 1 16,88 25
Serapan = 2,549 A
D. PERHITUNGAN
1. Penimbangan larutan standar (weighing by difference)

Bobot kloramfenikol standar = (wadah + kloramfenikol standar) (wadah + sisa

kloramfenikol standar)

= 241 mg 191 mg

= 50 mg

2. Pembuatan larutan induk

Perhitungan kadar larutan induk

bobot zat (mg)

Kadar larutan induk= .100 0
ml

50 mg
Kadar larutan induk= .100 0
25 ml

Kadar larutan induk =2000 ppm

3. Pembuatan seri konsentrasi larutan induk

M1.V1 = M2.V2
2000 ppm . 1 ml = 80 ppm . 25 ml

M1.V1 = M2.V2
80 ppm . 1 ml = 8 ppm . 10 ml

M1.V1 = M2.V2
80 ppm . 3 ml = 24 ppm . 10 ml

M1.V1 = M2.V2
2000 ppm . 1 ml = 200 ppm . 10 ml

M1.V1 = M2.V2
200 ppm . 1 ml = 20 ppm . 10 ml

M1.V1 = M2.V2
200 ppm . 2 ml = 16 ppm . 25 ml

M1.V1 = M2.V2
200 ppm . 3 ml = 60 ppm . 10 ml

M1.V1 = M2.V2
60 ppm . 2 ml = 12 ppm . 10 ml

M1.V1 = M2.V2
60 ppm . 3 ml = 18 ppm . 10 ml
4. Pembuatan kurva baku
Kadar (ppm) Serapan (A)
 8 2,543
12 2,575
16 2,568
18 2,566
20 2,69
24 2,58

Kadar vs Serapan
2.8
2.7

Serapan (A) 2.6 f(x) = 0x + 2.52

2.5 R = 0.23
2.4
6 8 10 12 14 16 18 20 22 24 26
Kadar (ppm)

Persamaan kurva baku

y = 0,0044x +2,5156

5. Preparasi sampel
2 gram 2000 mg mg
Kloramfenikol 2 = = =20
100 gram 100 gram gram

Sampel 1

Penimbangan sampel (weighing by addition)

Bobot sampel = (Wadah + sampel) Wadah

= 24920 mg - 23800 mg

= 1120 mg

Perhitungan kadar sampel

20 mg bobot sampel
=
1000 mg 1120 mg

B obot sampel=22,4 m g

22,4 mg
Kadar sampel= .100 0
50 ml

Kadar sampel=448 pp m
 Perhitungan pengenceran (FP=25)
M1.V1 = M2.V2
448 ppm . 1 ml = Kadar sampel . 25 ml
Kadar sampel = 17,92 ppm

Sampel 2

Penimbangan sampel (weighing by addition)

Bobot sampel = (Wadah + sampel) Wadah

= 24845 mg - 23800 mg

= 1045 mg

Perhitungan kadar sampel

20 mg bobot sampel
=
1000 mg 1045 mg

B obot sampel=20,9 m g

20,9 mg
Kadar sampel= .100 0
50 ml

Kadar sampel=418 pp m

Perhitungan pengenceran (FP=25)

M1.V1 = M2.V2
418 ppm . 1 ml = Kadar sampel . 25 ml
Kadar sampel = 16,72 ppm

Sampel 3

Penimbangan sampel (weighing by addition)

Bobot sampel = (Wadah + sampel) Wadah

= 24855 mg - 23800 mg

= 1055 mg

Perhitungan kadar sampel

20 mg bobot sampel
=
1000 mg 10 55 mg

B obot sampel=2 1,1 m g

 2 1,1 mg
Kadar sampel= .100 0
50 ml

Kadar sampel=422 pp m

Perhitungan pengenceran (FP=25)

M1.V1 = M2.V2
422 ppm . 1 ml = Kadar sampel . 25 ml
Kadar sampel = 16,88 ppm
6. Kadar kloramfenikol dalam sampel (salep)
A x 10 x Fp x V awal x 100
Kadar kloramfenikol dalam salep= x 100
E x mg sampel x 2

Sampel 1
2,569 x 10 x 25 x 50 x 100
Kadar kloramfenikol dalam salep= x 100
298 x 1120 x 2
Kadar kloramfenikol dalam salep= 481,072%

Sampel 2
2,550 x 10 x 25 x 50 x 100
Kadar kloramfenikol dalam salep= x 100
298 x 1045 x 2
Kadar kloramfenikol dalam salep= 511,785%

Sampel 3
2,549 x 10 x 25 x 50 x 100
Kadar kloramfenikol dalam salep= x 100
298 x 1055 x 2
Kadar kloramfenikol dalam salep= 506,735%

Rata-rata kadar = 499,864%

Standar Deviasi=
(xix )2
n1

Standar Deviasi=
542,477
2

Standar Deviasi=16,469

Kadar kloramfenikol dalam salep=ratarata kadar standar deviasi

Kadar kloramfenikol dalam salep=499,864 16,469

E. PEMBAHASAN
Monografi bahan
1. Kloramfenikol
2. Etanol
Cara kerja dan fungi perlakuan
Hasil vs literatur
F. KESIMPULAN
G. DAFTAR PUSTAKA