1964 5168 2 PB PDF
1964 5168 2 PB PDF
1 (1): 29-36
ABSTRAK
Latar Belakang: Monosodium glutamat (MSG) merupakan garam natrium dari asam glutamat (asam
amino non-esensial). Monosodium glutamat banyak digunakan sebagai penyedap rasa pada makanan.
Tujuan: untuk mengetahui efek MSG membentuk mikronukleus pada sel darah merah sumsum
tulang femur mencit.
Metode Penelitian: Makanan yang diberikan terhadap mencit adalah MSG yang dicampurkan ke
dalam pelet. MSG yang dicampurkan ke dalam pelet diberikan terhadap mencit dengan dosis 3, 6 dan
9 g/hari, selama 14 hari berturut-turut. Setelah itu mencit dibunuh untuk diambil sumsum tulang
femur dan dibuat preparat apusan. Aktivitas mutagenik ditunjukkan oleh adanya peningkatan jumlah
mikronukleus dalam setiap 200 sel eritrosit polikromatik pada preparat apusan sumsum tulang femur
mencit.
Hasil: Hasil analisis variansi (ANAVA) secara signifikan menunjukkan bahwa pemberian MSG
meningkatkan jumlah mikronukleus pada 200 sel eritrosit polikromatik yang terdapat pada apusan
sumsum tulang femur mencit dibandingkan terhadap kelompok kontrol (p < 0,05).
Kesimpulan: Pemberian MSG dosis 9 g/hari menunjukkan jumlah mikronukleus yang lebih banyak
dibandingkan dosis 3 dan 6 g/hari.
ABSTRACT
Background: Monosodium glutamate (MSG) is a sodium salt of glutamic acid (non-essential amino
acids). Monosodium glutamate is used in many food seasonings.
Objective: The purpose of this study was to determine the effect of MSG on the mice femur bone
marrow red blood cells micronucleus formation.
Methods: The food given to the mice was MSG added in pellets. MSG added in pellets were given to
the mice at dose of 3, 6 and 9 g/day for 14 consecutive days. Then the mice were killed for their femur
bone marrow and the smear preparations. Mutagenic activity was shown by the increasing number of
micronucleus in every 200 polychromatic erythrocytes cells in the femur bone marrow smear
preparation of mice.
Results: The analysis of variance (ANOVA) significantly showed that the administration of MSG
increased the number of micronucleus in every 200 polychromatic erythrocytes cell found in the
femur bone marrow smear of mice compared to the control group (p < 0.05).
Conclusion: The administration of MSG with dose of 9 g/day showed that the number of
micronucleus is more than the administration with dose of 3 and 6 g/day.
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Journal of Pharmaceutics and Pharmacology, 2012 Vol. 1 (1): 29-36
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Journal of Pharmaceutics and Pharmacology, 2012 Vol. 1 (1): 29-36
A B
C D
Gambar 1. Sel-sel yang diamati pada apusan sumsum tulang femur mencit
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Journal of Pharmaceutics and Pharmacology, 2012 Vol. 1 (1): 29-36
Keterangan gambar:
A : Sel eritrosit polikromatik bermikronukleus (MSG 3 g)
B : Sel eritrosit polikromatik bermikronukleus (MSG 6 g)
C : Sel eritrosit polikromatik bermikronukleus (MSG 9 g)
D : Sel eritrosit polikromatik tidak bermikronukleus (kontrol
normal) (Sumber: Sitorus, 2012)
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Gambar 2. Grafik hasil pengukuran jumlah rata-rata (n=6) mikronukleus pada 200 sel
eritrosit polikromatik.
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Journal of Pharmaceutics and Pharmacology, 2012 Vol. 1 (1): 29-36
Berdasarkan penelitian terhadap MSG Farombi, E.O., and Onyema, O.O. (2006).
yang dicampurkan dengan makanan hewan Monosodium Glutamat-Induced
berupa pelet dengan dosis 3, 6, 9 g/hari. Oxidative Damage and
Aktivitas mutagenik ditunjukkan oleh Genotoxicity in the Rat:
adanya peningkatan jumlah mikronukleus Modulatory Role of Vitamin C,
dalam setiap 200 sel eritrosit polikromatik Vitamin E and Quercetin. Human
pada preparat apusan sumsum tulang & Experimental Toxicology. 25(5):
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Taste That Kills. Albuquerque: D.J. (1977). Monosodium Glutamat
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2. Reduces Reproductive Ability in
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Chromosomal Stability of Some
Dietary Constituents. Dissertation. Prawirohardjono, W., Dwiprahasto, I.,
Uppsala: Uppsala Universited. Indwiani, A., Hadiwandowo, S.,
Kristin, E., Muhammad, M., dan
EPA. (1998). Health Effects Test Michael, F.K. (2000). The
Guidelines OPPTS 870.5395 Administration to Indonesians of
Mammalian Erythrocyte Monosodium L-glutamat in
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