Perbedaan Luas Implan Endometriosis, Ekspresi VEGF, Kadar SOD pada

Serum Darah Mencit Model Endometriosis Yang diberi Leuprolide Acetat,

Gliadin-ekstrak Melon, Kombinasi Leuprolide Acetat dengan Gliadin-
ekstrak Melon

PROPOSAL TESIS

Oleh:
dr. Fanni Priyo Nugroho

Pembimbing:
DR .dr. Syarief Thaufik Hidayat, MS.Med SpOG (K)

PROGRAM PENDIDIKAN DOKTER SPESIALIS 1

OBSTETRI DAN GINEKOLOGI FAKULTAS KEDOKTERAN
UNIVERSITAS DIPONEGORO
SEMARANG
2019
 HALAMAN PENGESAHAN

Judul Penelitian : Perbedaan Luas Implan Endometriosis, Ekspresi VEGF,

Kadar SOD Pada Serum Darah Mencit Model Endometriosis Yang Diberi
Leuprolide Acetat, Gliadin-ekstrak Melon, Kombinasi Leuprolide Acetat
Dengan Gliadin-ekstrak Melon

Ruang Lingkup : Obstetri dan Ginekologi

Pelaksana Penelitian : dr. Fanni Priyo Nugroho

Pembimbing : Dr. dr. Syarief Thaufik Hidayat, MS.MedSpOG(K)

Semarang, November 2019

Pembimbing

DR. dr. Syarief Thaufik Hidayat, , MS.Med SpOG(K)

NIP. 19641014 199011 1 002

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 DAFTAR ISI

HALAMAN PENGESAHAN.................................................................................i
DAFTAR ISI ..........................................................................................................ii
DAFTAR TABEL ..................................................................................................v
DAFTAR GAMBAR .............................................................................................vi
DAFTAR SINGKATAN ......................................................................................vii
BAB I

PENDAHULUAN..................................................................................................1
1.1 Latar Belakang...............................................................................................1
1.2 Rumusan Masalah..........................................................................................3
1.3 Tujuan Penelitian............................................................................................5
1.3.1 Tujuan Umum..........................................................................................5
1.3.2 Tujuan Khusus.........................................................................................5
1.4 Manfaat Penelitian..........................................................................................6
1.4.1 Manfaat Keilmuan...................................................................................6
1.4.2 Manfaat Pelayanan...................................................................................6
1.4.3 Manfaat Penelitian...................................................................................6
1.5 Keaslian Penelitian........................................................................................7
BAB II TINJAUAN PUSTAKA..............................................................................9
2.1 Endometriosis.................................................................................................9
2.1.1 Pengertian Endometriosis........................................................................9
2.1.2 Patogenesis..............................................................................................9
2.2 Enzim Superoside Dismutase (SOD)...........................................................11
2.2.1 Definisi..................................................................................................11
2.2.2 Reaksi Disproporsionasi Superoksida...................................................11
2.2.3 Kelas dan Struktur SOD........................................................................12
2.2.4 Fungsi SOD pada Tubuh Manusia........................................................14
2.2.5 Suplementasi SOD.................................................................................15
2.3 Vascular Endothelial Growth Factor (VEGF)..............................................16
2.4 Bentuk dan Ukuran Jaringan Endometriosis ...............................................18

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 2.5 Terbentuknya Implan Endometriosis..........................................................20
2.6 Kombinasi Gliadin dan Ekstrak Melon .......................................................22
2.6.1 Mekanisme kerja dan Farmakologi.......................................................22
2.6.2 Peran Kombinasi Gliadin dan Ekstrak Melon pada Proses Stres
Oksidatif.........................................................................................................24
2.6 Leuprolide Asetat ........................................................................................25
2.6.1 Mekanisme kerja dan Farmakologi.......................................................25
2.6.2 Keamanan Toleransi dan Efek Samping...............................................27
2.6.3 Leuprolide Acetat pada Endometriosis.................................................28
2.7 Penggunaan mencit sebagai model endometriosis……………………….29

2.8 Kerangka Patofisiologi….……………………… ……………………….31

2.9 Kerangka Konsep.……………………………… ……………………….32

2.10 Kerangka Teori…..…….……………………… ……………………….32

BAB III Hipotesis..................................................................................................33

3.1 Hipotesis Mayor.......................................................................................33
3.2 Hipotesis Minor........................................................................................33
BAB IV METODE PENELITIAN........................................................................34
4.1 Ruang lingkup Penelitian.............................................................................34
4.2 Tempat dan Waktu Penelitian......................................................................34
4.3 Jenis dan Rancangan Penelitian...................................................................34
4.4 Sampel Penelitian.........................................................................................34
4.4.1 Sampel...................................................................................................34
4.4.2 Cara Sampling.......................................................................................35
4.4.3 Besar Sampling......................................................................................35
4.5 Variabel Penelitian.......................................................................................36
4.6 Definisi Operasional.....................................................................................37
4.7 Alat, Bahan dan Prosedur Penelitian............................................................39
4.7.1 Alat........................................................................................................39
4.7.2 Bahan.....................................................................................................40
4.7.3 Cara Kerja..............................................................................................40

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 4.8 Alur Penelitian..............................................................................................46
4.9 Analisis Data................................................................................................47
4.10 Etika penelitian...........................................................................................47
DAFTAR PUSTAKA............................................................................................48

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 DAFTAR TABEL

Tabel 1. Keaslian Penelitian .............................................................................. 7

Tabel 2. Definisi Operasional............................................................................. 37

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 DAFTAR GAMBAR

Gambar 1. Struktur Cu,Zn-SOD ........................................................................ 11

Gambar 2. Struktur Mn-SOD............................................................................. 12

Gambar 3. Tipe VEGF dan reseptornya............................................................. 18

Gambar 4. Reaksi enzimatik radikal bebas oksigen menjadi air dan oksigen.... 23

Gambar 5. Berbagai penelitian kombinasi gliadin dan ekstrak melon .............. 24

Gambar 6. Representasi diagramatik interpretasi Allred score……………….. 39

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 DAFTAR SINGKATAN

CAT : Catalase

EGF : Epidermal Growth Factor

GnRH : Gonadotropin realizing hormone

MDGF : Macrophage Derived Growth Factor

MMP : metalloproteinase

MDA : Malondialdehide

O2 : Oksigen

RNS : Reactive Nitrogen Species

ROS : Reactive Oxygen Species

SOD : Superoxide Dismutase

VEGF : Vascular Endhotelial Growth Factor

IL-1β : Interleukin-1β

IL-1 RA : IL-1 reseptor antagonis

IL-2 : Interleukin-2

IL-10 : Interleukin-10

IL-16 : Interleukin-16

IL-18 : Interleukin-18

IFN-Υ : interferon

TNF- α : tumor nucleus factor α

TNF- β : tumor nucleus factor β

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