SPEKTROSKOPI

FLUORESENSI
Purri Ardelia N / A 171 094
Pengertian

Spektroskopi
adalah ilmu yang mempelajari materi dan
atributnya berdasarkan cahaya, suara atau
partikel yang dipancarkan, diserap atau
dipantulkan oleh materi tersebut. Spektroskopi
juga dapat didefinisikan sebagai ilmu yang
mempelajari interaksi antara cahaya dan materi.

2
Spektrofluorometri
metode analisis kimia kuantitatif yang
berdasarkan fluoresensi.

Fluoresensi
proses pemancaran radiasi cahaya oleh
suatu materi setelah tereksitasi oleh
berkas cahaya berenergi tinggi. Molekul
tereksitasi akan kembali pada keadaan
dasar atau ke tingkat eksitasi lebih
rendah, dengan mengemisikan sinar.
Sinar yang diemisikan inilah yang akan
3
diukur.
 prinsip
1. Eksitasi
Cahaya diserap oleh molekul, yang ditransfer
ke keadaan tereksitasi secara elektronik
yang berarti bahwa sebuah elektron
bergerak dari keadaan dasar singlet

2. Relaksasi Getaran
Molekul mengalami transisi dari elektronik atas
menjadi lebih rendah
3. Emisi
Elektron kembali ke keadaan dasar lebih stabil.
Memancarkan cahaya sesuai panjang
gelombang yang sesuai
Instrumentasi

5

1. Sumber energi eksitasi :
2. Kuvet untuk sampel
lampu xenon 75 – 450
W
Emisi 300 - 1300 nm
: * Kuvet silika
* Kuvet dari gelas

3. Detektor :
Detektor fluorescence memiliki
sensitivitas 10 – 10.000 kali lebih
kuat dibanding detektor UV

4. Monokromator : Monokromator 1
Menyediakan radiasi yang cocok untuk eksitasi
molekul
Monokromator 2
Memisahkan hanya radiasi yang dipancarkan
6
oleh molekul fluororescent
 Jenis senyawa yang di analisis

1 2 3
Harus mengandung gugus Struktur molekulnya planar dan Struktur molekul yang
kromofor. Contohnya adalah rigid/kaku mempunyai ikatan rangkap
senyawa-senyawa aromatik, mempunyai sifat fluoresensi
heterosiklik, dan sistem karena strukturnya kaku dan
konjugasi planar

7
 Big concept
Bring the attention of your audience
over a key concept using icons or
illustrations
8
 In two or three columns
Yellow Blue Red
Is the color of gold, Is the colour of the Is the color of blood,
butter and ripe clear sky and the and because of this it
lemons. In the deep sea. It is has historically been
spectrum of visible located between associated with
light, yellow is found violet and green on sacrifice, danger and
between green and the optical spectrum. courage.
orange.

9
A picture is
worth a
thousand words
A complex idea can be
conveyed with just a
single still image,
namely making it
possible to absorb
large amounts of data
quickly.

10
Want big impact? Use big
image.

11
 Use diagrams to explain your
ideas

LOREM 3 LOREM 1
Vestibulum nec congue Vestibulum nec congue
tempus lorem ipsum tempus lorem ipsum

Vestibulum
nec congue
tempus

LOREM 2
Vestibulum nec congue
tempus lorem ipsum
12
And tables to compare data

A B C

Yellow 10 20 7

Blue 30 15 10

Orange 5 24 16

13
 Maps

our office

Find more maps at slidescarnival.com/extra-free-resources-icons-and-maps

14
89,526,124
Whoa! That’s a big number, aren’t you proud?

15
 89,526,124$
That’s a lot of money

185,244 users
And a lot of users

100%
Total success!

16
 Let’s review some concepts
Yellow
Is the color of gold, butter and
ripe lemons. In the spectrum
of visible light, yellow is found
between green and orange.
Yellow
Is the color of gold, butter and
ripe lemons. In the spectrum
of visible light, yellow is found
between green and orange.
Blue
Is the colour of the clear sky
and the deep sea. It is located
between violet and green on
the optical spectrum.
Blue
Is the colour of the clear sky
and the deep sea. It is located
between violet and green on
the optical spectrum.
17
Red
Is the color of blood, and
because of this it has
historically been associated
with sacrifice, danger and
courage.
Red
Is the color of blood, and
because of this it has
historically been associated
with sacrifice, danger and
courage.
You can insert graphs from Excel or Google Sheets

18
 Place your
screenshot here

Mobile project
Show and explain your web, app or software projects using these gadget templates.

19
 Place your screenshot here

Tablet project
Show and explain your web, app or software projects using these gadget templates.

20
 Place your screenshot here

Desktop project
Show and explain your web, app or software projects using these gadget templates.

21
 THANKS!
Any questions?
You can find me at @username & user@mail.me

22
 Credits
Special thanks to all the people who made
and released these awesome resources for
free:
✘ Presentation template by SlidesCarnival
✘ Photographs by Unsplash

23
 Presentation design
This presentation uses the following typographies:
✘ Titles: Amatic SC
✘ Body copy: Quicksand
Download for free at:
https://www.fontsquirrel.com/fonts/amatic
https://www.fontsquirrel.com/fonts/quicksand

You don’t need to keep this slide in your presentation. It’s only here to serve you as a design
guide if you need to create new slides or download the fonts to edit the presentation in
PowerPoint®

24
 SlidesCarnival icons are editable
shapes.

This means that you can:

✘ Resize them without losing
quality.
✘ Change fill color and opacity.
✘ Change line color, width and
style.

Isn’t that nice? :)

Examples:

Find more icons at

slidescarnival.com/extra-free-resources-ic
ons-and-maps

25
 You can also use any emoji as an icon!

😉
And of course it resizes without losing quality.
How? Follow Google instructions
https://twitter.com/googledocs/status/730087240156643328

✋👆👉👍👤👦👧👨👩👪💃🏃💑❤😂😉😋😒😭
👶😸🐟🍒🍔💣📌📖🔨🎃🎈🎨🏈🏰🌏🔌🔑and many
more...

26
 Free templates for all your presentation needs

For PowerPoint and 100% free for Ready to use, Blow your audience
Google Slides personal or professional and away with attractive
commercial use customizable visuals