KROMOTOGRAFI

EKSLUSI
OLEH KELOMPOK 4:
 A.ULFA/1913142011
 NURMILA
 HERMITA PATANDEAN
 NUR AZIZAH FITRIYAH
 KROMOTOGRAFI EKSLUSI

01. DEFENISI
Size exclusion chromatography (SEC) disebut juga gel
permeation chromatography (GPC), gel filtration
chromatography (GFC), merupakan metode kromatografi
yang menggunakan partikel berpori untuk memisahkan
molekul dengan ukuran yang berbeda.
SEC umumnya diaplikasikan untuk kompleks
makromolekuler sperti protein dan industri polimer
terutama digunakan untuk memisahkan molekul biologis,
untuk menentukan bobot molekul dan distribusi bobot
molekul dari polime.

R
02. PRINSIP KERJA
Size-Exclusion Chromatography (SEC) disebut juga Gel
Permeation Chromatography (GPC), didasarkan pada pemisahan
molekul polimer sesuai dengan volume hidrodinamikanya ketika
sampel diinjeksikan ke dalam kolom dengan meninjau perbedaan
ukuran dan geometri moleku
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