Dr.DWI WINARNI, M.

Si
Dept. BioIogi
FSaintek Univ. AirIangga
BioIogi SeI - pertemuan IV
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SEL
HIDUP
Non
SELULAR
(ECM)
SELULAR
LINGKUNGAN
INTERAKSI
Komponen permukaan
seI
MENENTUKAN
BENTUK/JENIS
L|gan ter|arut
(soluble ligand}
hormon, growth factor
Hatr|ks ekstrase|
(fixed ligand}
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ANG BERPERAN DALAM INTERAKSI SEL
DENGAN LINGKUNGANNA
1. INTEGRINS
2.SELECTINS
3. IMMUNOGLOBULINS
superfamiIy
4. CADHERINS
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Merupakan keIompok protein integraI membran
yang terdapat pada permukaan seI vertebrata
Tersusun atas 2 rantai poIipeptida, 1 rantai dan
1 rantai yang terangkai secara nonkovaIen
16 jenis rantai
jenis rantai
22 heterodimer
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Rantai
meningkatkan
spesifitas
pengikatan Iigan
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1. ADESI SEL PADA
SUBSTRAT/ SEL
2. TRANSMISI SINAL
DARI EKSTRASEL
(outside-in signaling)
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SEBAGIAN BESAR PROTEIN EKSTRASELULAR
BERIKATAN DENGAN INTEGRIN PADA BAGIAN
ANG MENGANDUNG URUTAN (SEKUENS)
ASAM AMINO arginin-gIisin-asam aspartat
(nomenkIatur baru= RGD),
dengan ligand -binding site tergantung pada
keberadaan ion Ca2+ atau Mg2+
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Jenis
reseptor
Distribusi Jenis Iigan

ll
3e| T lera|l|vas|, l|ros|l, erdole|,
l|oroo|as, ep|le|, aslros|l
ibronektin

ll
3e| T dar 8 lera|l|vas|, se| T
reror|, l|ros|l, l|oroo|as, ep|le|,
p|ale|els, aslros|l, erdole|
ibronektin

llb

11
3e| T dar 8 lera|l|vas|, se| T
reror|, l|ros|l, l|oroo|as, ep|le|,
p|ale|els, erdole|
ibronektin
Vitronektin
ibrinogen
Von Willebbrand factor

F|oroo|as, roros|l, ra|rolag,

ep|le|, se| luror
Vitronektin
BEBERAPA JENIS RESEPTOR INTEGRIN DAN JENIS LIGAN ANG
DIKENAL MELALUI SEKUENS RGD
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Jenis
reseptor
integrin
Distribusi Jenis Iigan

ll
NKC, se| T dar 8 lera|l|vas|, l|oroo|as, se| g||a, per|rer|ur,
se| 3crWarr, se| eperd|r
Ko|ager

ll
NKC, se| T dar 8 lera|l|vas|, p|ale|el, erdole|, ep|le|,
aslros|l, l|oroo|as, se| 3crWarr, se| eperd|r
Ko|ager
Lar|r|r

ll
3e| T dar 8 lera|l|vas|, l|ros|l, erdole|, l|oroo|as, ep|le|,
aslros|l
Ko|ager
Lar|r|r

ll
NKC, se| T dar 8 lera|l|vas|, eos|rol||, erdole|, olol,
l|oroo|as
F|ororecl|r
vCAV
BEBERAPA JENIS RESEPTOR INTEGRIN DAN JENIS LIGAN
ANG DIKENAL MELALUI SEKUENS nonRGD
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INTEGRIN MENGIKATKAN SEL PADA SUBSTRAT
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$e| da|am b|akan secara d|skr|t mengadakan per|ekatan pada permukaan. Kontak
|ntegr|n (} dengan prote|n d| substrat yang me|ap|s| permukaan (m|s: |am|n|n,
ko|agen, f|bronekt|n} perubahan konformas| |ntegr|n s|top|asm|k per|ekatan
|ntegr|n dengan f||amen akt|n penge|ompokan (clustering} |ntegr|n d| permukaan
FOCAL CONTACT /
FOCAL ADHESIONS
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LM= Iaminin
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ocal contact merupakan
struktur yang dinamik, yang
seteIah terbentuk, secara
cepat akan "terurai" jika seI
daIam biakan terstimuIasi
untuk mitosis atau
perubahan organisasi
sitoskeIeton yang Iain
biosel_S1_bio HEMIDESMOSOM
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SeI dapat
mengekspresikan
berbagai jenis
integrin pada
permukaannya
SeI dapat mengikat
berbagai jenis
komponen matriks
ekstraseIuIar
3e|-se| oasa| rerge|spres||ar |rlegr|r
2l, 1l dar l yarg oerperar
da|ar ||alar desrosor dergar
|orporer rerorara oasa||s
3e| oasa| ep|derr|s
rerupa|ar salu-salurya
jer|s se| da|ar |ap|sar
ep|le| ep|derr|s yarg
rerpurya| |erarpuar
reroe|ar, j||a s|rles|s
|rlegr|r l|da| oerrerl| saal
se| rer|rgga||ar oasa|
se| a|ar lerus reroe|ar
|ord|s| r|r|p psor|as|s
(ep|derr|s rereoa| dar
rerga|ar| peradargar)
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BuIIous pemphigoid
disebabkan oleh terbentuknya autoantibodi terhadap
struktur hemidesmosom sel epitel bagian basal tidak
dapat melekat pada membrana basalis dan jaringan ikat
di bawahnya terisi cairan tubuh bula, terutama
nampak pada kulit
Epidermolysis bulosa
disebabkan oleh faktor genetis (perubahan pada protein
penyusun hemodesmosom termasuk subunit o atau 4
integrin atau laminin bula (termasuk pada dinding
saluran gastrointestinal dan urin)
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Merupakan keIompok gIikoprotein integraI
membran yang dapat mengenaIi dan mengikat
gugus guIa tertentu yang terdapat pada
permukaan seI Iain
Nama seIectin berasaI dari Iectin yaitu senyawa
yang dapat mengikat gugus guIa tertentu secara
spesifik pengikatan seIektin pada Iigannya
memerIukan ion kaIsium
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SELECTINS
E-SELECTINS
CD62e
P-SELECTINS
CD62p
L-SELECTINS
CD62I
SEL-SEL
ENDOTEL
!%%$
ENDOTEL
SEMUA
JENIS
LEKOSIT
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T cellendothelial-cell interactions as studied in flow chamber systems 3
;974. P-selectin-dependent interactions require functional P-selectin
glycoprotein ligand 1 (PSGL1); E-selectin interactions require poorly defined
E-selectin ligand(s) on acti;ated T cells. L-selectin on T cells interacts with
peripheral node addressin (PNAD). At sites of inflammation, endothelial cells
express P- and E-selectin and during chronic inflammation PNAD is also
expressed by endothelial cells
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At sites of inflammation, neutrophils and monocytes, or neutrophil- or monocyte-
deri;ed microparticles, interact with the inflamed endothelium and present
functional PSGL1 to T cells. This PSGL1 can interact with L-selectin on nai;e or
central memory T cells. Acti;ated platelets and platelet-deri;ed microparticles are
also known to interact with the ;ascular endothelium and can present P-selectin to
T cells. Note that for simplicity, not all domains of the selectins are depicted,
although they are represented according to their relati;e sizes.
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A schematic of
the structure of
the T-ceII
receptor (TCR)
Antigen presentation stimuIates T ceIIs to
become either "cytotoxic" CD+ ceIIs or
"heIper" CD4+ ceIIs
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Tre |oW-all|r|ly lgE receplor, Fc Rll (C023), |s expressed oy a W|de var|ely ol |rrure
ce|| lypes, |rc|ud|rg 8 ce||s ard derdr|l|c ce||s. lgE lral |s oourd lo Fc Rl or Fc Rll
car lac|||lale a||erger upla|e oy arl|ger-preserl|rg ce||s (APCs) ard augrerl
secordary |rrure resporses. b Vosl lgE |s oourd oy |ls r|gr-all|r|ly receplor, Fc
Rl, expressed oy rasl ce||s ard oasopr||s. Cross||r||rg ol lgE oourd lo Fc Rl or
l|ssue rasl ce||s oy spec|l|c arl|ger resu|ls |r lre |oca| re|ease ol |rl|arralory
red|alors (lor exarp|e, r|slar|re ard |eu|olr|eres), erzyres ard cylo||res lral
red|ale lre c||r|ca| rar|leslal|ors ol alopy.
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Neura| ce|| adres|or ro|ecu|e
(NCAV) ard L1 rave |rporlarl
ro|es |r ce||-ce|| |rleracl|ors
lrrougr roropr|||c (NCAV-
NCAV or L1-L1) ard
releropr|||c (NCAV-L1)
o|rd|rg. Trey allecl
reuroc|rcu|lry (pare| b), ard, oy
|rleracl|rg W|lr cylos|e|ela|
corporerls, lrey car acl|vale
spec|l|c |rlrace||u|ar s|gra|||rg
palrWays. Trese ro|ecu|es
parl|c|pale |r reur|le exlers|or
ard gu|darce, ce||
d|llererl|al|or ard surv|va|, ard
syraplogeres|s. lr add|l|or lo
lre|r p|vola| ro|es |r reura|
deve|oprerl ard regereral|or,
lrey rave oeer slrorg|y
|rp||caled |r syrapl|c p|asl|c|ly
ard rerory lorral|or
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- DITEMUKAN SECARA LUAS DI PERMUKAAN SEL
- UMUMNA DIMER
- BAGIAN EKSTRASEL MERUPAKAN STRUKTUR TANDEM
BERULANG 5
- BAGIAN INTRASEL BERIKATAN DENGAN PROTEIN SITOPLASMIK
CATENIN CATENIN BERIKATAN DENGAN AKTIN SITOSKELETON
- Jenis:
1. yang berinteraksi dengan sitoskeIeton
(Cadherin N, P, R, B, dan E)
2. berasosiasi dengan desmosom (desmogIein, desmocoIin)
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Tre ep|lre||a|-resercryra| lrars|l|or (EVT) ard |ls reverse lre resercryra|-ep|lre||a|
lrars|l|or
.
E-cadrer|r (ep|lre||a| cadrer|r) red|ales lre adres|or ol ce||s, lre lrars|l|or ol ce||s
lror orgar|zal|or |r a |oose resercryra| relWor| |r Wr|cr lrey |ac| po|ar|ly ard rave
r|gralory ard |rvas|ve polerl|a| lo lre|r l|grl appos|l|or |r a po|ar|zed ep|lre||a| oarr|er, ard
lre lorral|or ol l|grl jurcl|ora| corp|exes. Loss ol E-cadrer|r express|or |s assoc|aled W|lr
lre EVT ard |ls |oss or dysregu|al|or p|ays a parl |r lurour ce|| |rvas|or ard relaslas|s. Tre
ye||oW ce||s express E-cadrer|r, |r corlrasl lo lre o|ue ce||s lral do rol
Peran chaderin daIam morfogenesis
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CeII sorting owing to differentiaI expression of cadherins drives
tissue separation during embryonic deveIopment
.
Expression of N-
cadherin (neuraI cadherin; bIue) in the presumptive neuraI
epitheIium aIIows it to separate from the E-cadherin-expressing
ectoderm (yeIIow)
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The dynamic breaking and reforming of cadherin adhesi;e
bonds is required for cells to change neighbours despite being
held together in the tissue. n the example shown, called
con;ergence and extension
,
cells rearrange in such a way as to
cause the tissue to narrow and elongate.
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ormation of compartment boundaries
.
Selecti;e adhesion, which is
accomplished through regulation of adhesi;e strength, creates
boundaries between de;elopmental compartments in a tissue, as in
the formation of rhombomeres in the de;eloping ;ertebrate hindbrain
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Growth cone motiIity and synapse formation. N-cadherin (and
other cadherins) can mediate growth cone motiIity aIong ceIIs that
express N-cadherin. Cadherins aIso form adherens-type junctions
as part of the synaptic junction, which is important for synaptic
specificity, reguIation of synaptic pIasticity and dendrite
morphogenesis
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Ce|| r|gral|or. lr corlrasl lo |ls ro|e |r slao|||z|rg ep|lre||a| jurcl|ors, E-
cadrer|r a|so red|ales lre |org-rarge r|gral|or ol ce||s lrrougr l|ssues; lor
exarp|e, lre r|gral|or ol oorder ce||s (p|r|) |r lre 0rosopn||a
me|anoasrer egg craroer
.
Tre oorder ce||s r|grale lror ore erd ol lre egg
craroer oelWeer lre rurse ce||s url|| lrey reacr lre oocyle.
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