TPE4211

RANCANGAN
ALAT BIOPROSES
 DOSEN PENGASUH:

YUSRON SUGIARTO
WAHYUNANTO AGUNG NUGROHO
 TUJUAN KULIAH
Mahasiswa mempunyai kemampuan menterjemahkan
dan memahami perancangan berbagai tipe bioreaktor
meliputi bentuk bioreaktor, proses, kondisi operasi, dan
susunan bioreaktor, serta pemilihan tipe bioreaktor
 MATERI KULIAH
No Pokok Bahasan Sub Pokok Bahasan Waktu
(Jam)
1 3 4 5
1. Pengantar Overview Bioreaktor 2 x 50
2. Tahap Inokulasi Dasar mikroorgmisme 2 x 50
kurva pertumbuhan
3. Dasar pemodelan reaktor Modelling, matematis monod 2 x 50
4. Konfigurasi Bioreaktor Stirred Tank 2 x 50
Bubble colomn
5. Konfigurasi Bioreaktor Airlift Reactor 2 x 50
Stirred and Air Driven Reactor
6. Konfigurasi Bioreaktor Packed Bed 2 x 50
Trickle Bed
7. Konstruksi Bioreaktor Aseptic Operation 2 x 50
Fermenter inoculation and
sampling
Materials of construction
Sparger design
Evaporation control
 MATERI KULIAH (2)
No Pokok Bahasan Sub Pokok Bahasan Waktu
(Jam)
1 3 4 5
8. Monitoring and Control of Fermentation monitoring 2 x 50
Bioreactor (1) Measurement analysisi
Fault analysis
Process modelling
State estimation
9. Monitoring and Control of Feed back control 2 x 50
Bioreactor (2) Programmed controlled
Application of Artificial Intelegence in
Bioprocess control
10. Ideal Reactor Operation Batch operation of a mixed reactor 2 x 50
Enzyme reaction
Cell culture, Chemostat with immobilised cells
Chemostat with cell recycle
Continues operation of a lug-flow reactor
11. Sterilisasi Batch heat sterilisation of liquids 2 x 50
Continues heat sterilisation of liquids
Filter sterilisation of Liquids
Sterilisation of air
12. Peningkata Skala Bioreaktor Peningkata Skala Bioreaktor 2 x 50
13. Pengembangan reaktor baru (1) Pengembangan reaktor biogas, bioethanol 2 x 50
14. Pengembangan reaktor baru (2) Pengembangan reaktor baru biodiesel 2 x 50
 3 SKS (2-1)
UTS : 30%
UAS : 30%
TUGAS (1) : 10%
TUGAS (2) : 10%
PRAKTIKUM : 20%
INTRODUCTION TO
BIOREACTOR SYSTEM
 What is BIOREACTOR?

What is the different between

REACTOR and BIOREACTOR?
REACTOR
reactors are vessels designed to contain chemical reactions.
One example is a pressure reactor. The design of a chemical
reactor deals with multiple aspects of chemical engineering.
Chemical engineers design reactors to maximize net present
value for the given reaction.

BIOREACTOR
A reactor system/vessel used for the cultivation of an
organism to produce the organism or a product, or in some
specialized cases to carry out specific reactions.

Main industrial equipment for fermentation process.

Major Function of
Bioreactor

To provide controlled
environment for growth of
microorganism (or mixture)
to obtain desired product.
Other functions
of a Bioreactor
1. Provide operation free from contamination
2. Maintain a specific temperature
3. Provide adequate mixing and aeration
4. Control the pH of the culture
5. Allow monitoring and/or control of dissolved
oxygen
6. Allow feeding of nutrient solutions
7. Provide access points for inoculation and
sampling
8. Minimize liquid loss from the vessel
9. Facilitate the growth of a wide range of organisms
Points to be considered in designing and constructing bioreactor:
1. Microbiological and biochemical characteristics of cell systems.
2. hydrodynamic characteristics of the bioreactor.
3. Mass and heat characteristics of the bioreactor.
4. kinetic of cell growth and product formation.
5. Genetic stability characteristics of the cell system.
6. Aseptic equipment design.
7. Control of bioreactor environment (macro and
microenvironments).
8. Implication of bioreactor design on downstream product
separation.
9. Capital and operating costs of the bioreactor.
10. Potential for bioreactor scale up.
 In any process, the bioreactor should not regarded as
an isolated unit but as part of integrated process
(include upstream and downstream processes)

strain isolation, storage, growth and preparation of inoccula as

Upstream well as sterilization of the bioreactor and the medium.

involve the extraction and purification of the products.

Downstream 20-60% of the total manufacturing costs
 Basic elements for control of a bioreactor

pH
Temperature
DO (dissolved oxygen)
Air flow
Agitation
Major components of an industrial bioreactor
Major components of an industrial bioreactor
Major components of an industrial bioreactor
 Diagram of a simple lab continuous fermentor

Sterile medium is passed into the bioreactor at a steady flow rate,

and culture broth emerges from it at the same rate, keeping the
volume of the total culture in the bioreactor constant.
 Optimization of production processes
1. Strain improvement
(Classical and recombinant DNA methods)

2. Fermentation process optimization

3. Downstream process optimization

Vary in size and
complexity,
test tube (10 ml) to
computer controlled
fermenters (>100 m3).
 Fermentor Scale Up
Can not start cell culture in 100,000 L, must
do repeated, scaled inoculations
Start with stock culture (5-10 mL)
Then shaker flask (200-500 mL)
Then seed fermentor (10L to 100 L)
Then production fermentor
(1000 L to 100,000 L)
Stages involved in a typical
manufacturing-scale fermentation

1L 10 L-50 L 5K-50K L
Shake Flask Incubator
Shake Flask Incubator
All Cells Can be Grown in
Incubators or Fermenters
 Four basic bioreactor designs:
Stirred tank reactors (mechanical agitation
for aeration)
Bubble column reactors (bubbling air into
media for aeration)
Internal loop airlift reactors (air and media
circulate together)
External loop airlift reactors
 Four Bioreactor Designs
A: Stirred tank

B: Bubble column

C: Internal-loop
airlift reactor

D: External-loop
airlift reactor
 Main parts of a benchtop fermentor
1) Mechanical: drive motor, heater, pump, gas control
2) Vessel and accessories
3) Peripheral equipment such as reagent bottles
4) Instrumentation and sensors 1

2
3
Anatomy of a benchtop bioreactor
Lab Scale Fermentor
Industrial Scale Fermentor
Industrial Scale Fermentor
THANK YOU...