93
94
Senyawa
Pereaksi Perubahan Warna Hasil
Golongan
Alkaloid Meyer Terbentuk endapan merah +++
Terpenoid Lieberman-Burchard Berwarna merah ++
Steroid Lieberman-Burchard Tidak berubah warna -
Saponin Uji busa + H2SO4 Terbentuk busa ++
Fenolik FeCl3 5% Berwarna hijau kebiruan +
Flavonoid HCl pekat + serbuk Mg Berwarna merah ++
Senyawa
Pereaksi Perubahan Warna Hasil
Golongan
Alkaloid Meyer Terbentuk endapan merah +++
Terpenoid Lieberman-Burchard Berwarna merah +
Steroid Lieberman-Burchard Tidak berubah warna -
Saponin Uji busa + H2SO4 Terbentuk busa +
Fenolik FeCl3 5% Berwarna hijau kebiruan +++
Flavonoid HCl pekat + serbuk Mg Berwarna merah +++
95
Larutan DPPH 100 g/mL diperoleh dengan mengencerkan larutan stok 1590
g/mL dalam labu ukur 25 mL, dengan rumus pengenceran sebagai berikut:
M1 x V1 = M2 x V2
1590 V1 = 2500 mL
V1 = 1,57 mL
M1 x V1 = M2 x V2
100g/mL x V1 = 40 g/mL x 50 mL
100 V1 = 2000 mL
V1 = 20 mL
96
Lampiran 4: Perhitungan Nilai IC50 pada Ekstrak Etanol Bunga Pacar Air
1. Perhitungan Nilai IC50 pada Ekstrak Etanol Bunga Pacar Air Merah
Ditimbang sebanyak 0,10269 g ekstrak etanol bunga pacar air merah dan
10.269 g/mL.
Larutan ekstrak etanol bunga pacar air merah 1000 g/mL diperoleh dengan
mengencerkan larutan stok 10.269 g/mL dalam labu ukur 10 mL, dengan rumus
M1 x V1 = M2 x V2
10.269 V1 = 10.000 mL
V1 = 0,9738 mL = 973,8 L
Pembuatan larutan dengan variasi konsentrasi (50, 100, 150, 200, 250, 300,
M1 x V1 = M2 x V2
1000 V1 = 250 mL
V1 = 0,25 mL = 250 L
% Peredaman =
y = 0,1546x 0,5564
nilai IC50, y = 50
50 = 0,1546x 0,5564
50 + 0,5564 = 0,1546x
50,5564 = 0,1546x
g/mL.
Larutan ekstrak etanol bunga pacar air merah 1000 g/mL diperoleh dengan
mengencerkan larutan stok 10.158 g/mL dalam labu ukur 10 mL, dengan rumus
M1 x V1 = M2 x V2
10.158 V1 = 10.000 mL
V1 = 0,9844 mL = 984,4 L
99
Pembuatan larutan dengan variasi konsentrasi (50, 100, 150, 200, 250, 300,
M1 x V1 = M2 x V2
1000 V1 = 250 mL
V1 = 0,25 mL = 250 L
% Peredaman =
y = 0,1546x 0,5564
nilai IC50, y = 50
50 = 0,1546x 0,5564
50 + 0,5564 = 0,1546x
50,5564 = 0,1546x
ANOVA
pH
Sum of Mean Kesimpulan
Squares df Square F Sig.
Between Groups 0.102 2 0.051 5.871 0.039 Karena nilai Sig.
Within Groups 0.052 6 0.009 < 0.05 maka hasil
Total 0.154 8 dikatakan
berbeda nyata
Homogeneous Subsets
pH
Duncana
Subset for alpha = 0.05
kelompok
N 1 2
FCPMG 3 4.9967
FPM 3 5.1633 5.1633
FG 3 5.2533
Sig. 0.071 0.281
102
ANOVA
L*
Sum of Squares df Mean Square F Sig. Kesimpulan
Between Groups 21.392 2 10.696 9.101 0.015 Karena nilai
Within Groups 7.052 6 1.175 Sig. < 0.05
Total 28.444 8 maka hasil
dikatakan
berbeda nyata
Homogeneous Subsets
L*
Duncana
Homogeneous Subsets
C
Duncana
a. Hasil analisa ukuran partikel dan indeks polidispersi hand and body cream
No. Sampel Ulangan Ukuran Indeks SD
Partikel Polidispersi
(IP)
1 FPM 1 778,9 333,996 0,827 0,226
2 454,6 0,869
3 1122,5 1,237
2 FG 1 1902,2 577,183 0,756 0,088
2 896,2 0,911
3 908,9 0,905
3 FC 1 1053,6 233,609 1,029 0,161
2 633,4 1,287
3 666,6 1,324
Perlakuan
Panelis
FPM FG FCPMG
1 2 2 2
2 2 4 3
3 2 1 1
4 2 3 2
5 3 4 3
6 2 2 2
7 2 3 2
8 1 3 1
9 2 2 3
10 2 2 2
11 4 3 2
12 3 1 3
13 3 3 3
14 3 2 2
15 4 3 4
16 3 2 3
17 3 3 3
18 3 3 4
19 1 1 1
20 2 2 1
21 3 3 3
22 3 3 3
23 3 3 3
24 3 4 4
25 2 2 3
26 2 3 3
27 2 2 3
28 3 3 3
29 2 1 3
30 3 4 3
Rata-rata 2.50 2.57 2.60
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
FPM 30 2.50 0.731 1 4
FG 30 2.57 0.898 1 4
FCPMG 30 2.60 0.855 1 4
Friedman Test
Ranks
Mean Rank
FPM 1.92
FG 2.02
FCPMG 2.07
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 0.667 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.717
a. Friedman Test
Test Statisticsc
FG - FPM FPM - FCPMG FCPMG - FG
Z -.423a -.775b -.221a
Asymp. Sig. (2-tailed) .672 .439 .825
a. Based on negative ranks.
b. Based on positive ranks.
c. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 4 3 3
2 3 4 3
3 3 2 3
4 3 3 4
5 3 4 3
6 3 2 3
7 3 2 2
8 3 3 3
9 3 4 3
10 3 3 3
11 4 2 3
12 4 1 3
13 4 3 4
14 2 2 3
15 2 4 3
16 3 3 4
17 3 4 4
18 4 3 4
19 2 2 2
20 4 3 2
21 3 3 3
22 4 4 4
23 3 4 3
24 3 4 4
25 4 3 4
26 4 4 4
27 3 2 2
28 3 2 4
29 3 3 4
30 5 3 4
Rata-rata 3.27 2.97 3.27
Descriptive Statistics
N Minimum Maximum Mean Std. Deviation
FPM 30 2.00 5.00 3.2667 0.69149
FG 30 1.00 4.00 2.9667 0.85029
FCPMG 30 2.00 4.00 3.2667 0.69149
Friedman Test
Ranks
Mean Rank
FPM 2.08
FG 1.78
FCPMG 2.13
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 3.308 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.191
a. Friedman Test
Test Statisticsd
FG - FPM FPM - FCPMG FCPMG - FG
Z -1.432a 0.000b -1.806c
Asymp. Sig. (2-tailed) 0.152 1.000 0.071
a. Based on positive ranks.
b. The sum of negative ranks equals the sum of positive ranks.
c. Based on negative ranks.
d. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 4 4 4
2 3 3 3
3 3 2 3
4 3 4 4
5 4 4 4
6 5 2 4
7 4 3 3
8 4 4 4
9 3 3 3
10 3 4 3
11 4 4 4
12 4 4 4
13 4 4 4
14 4 4 4
15 4 4 4
16 3 3 3
17 3 3 3
18 3 3 4
19 2 3 3
20 2 3 3
21 3 3 3
22 3 4 3
23 4 4 4
24 5 4 5
25 4 4 4
26 3 4 3
27 4 4 4
28 3 4 3
29 3 3 3
30 4 4 5
Rata-rata 3.5 3.53 3.6
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
FPM 30 3.5000 0.73108 2.00 5.00
FG 30 3.5333 0.62881 2.00 4.00
FCPMG 30 3.6000 0.62146 3.00 5.00
Friedman Test
Ranks
Mean Rank
FPM 1.90
FG 2.03
FCPMG 2.07
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 1.400 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan tidak
berbeda nyata
Asymp. Sig. 0.497
a. Friedman Test
Test Statisticsc
FG - FPM FPM - FCPMG FCPMG - FG
Z -.535a -1.134b -.577a
Asymp. Sig. (2-tailed) 0.593 0.257 0.564
a. Based on negative ranks.
b. Based on positive ranks.
c. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 4 4 4
2 2 5 2
3 3 4 4
4 4 4 4
5 4 4 4
6 4 3 3
7 3 4 4
8 2 1 1
9 4 4 3
10 3 3 3
11 3 3 3
12 3 3 3
13 3 3 3
14 2 2 2
15 4 4 3
16 4 3 3
17 3 3 3
18 3 3 3
19 3 4 4
20 3 3 3
21 4 3 3
22 5 5 5
23 4 4 4
24 4 4 4
25 4 4 4
26 5 5 5
27 4 4 4
28 3 4 2
29 3 3 3
30 4 4 5
Rata-rata 3.47 3.57 3.37
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
FPM 30 3.4667 0.77608 2.00 5.00
FG 30 3.5667 0.85836 1.00 5.00
FCPMG 30 3.3667 0.92786 1.00 5.00
Friedman Test
Ranks
Mean Rank
FPM 2.03
FG 2.07
FCPMG 1.90
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 1.514 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.469
a. Friedman Test
Test Statisticsc
FG - FPM FPM - FCPMG FCPMG - FG
Z -.577a -.905a -1.511b
Asymp. Sig. (2-tailed) 0.564 0.366 0.131
a. Based on negative ranks.
b. Based on positive ranks.
c. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 4 4 4
2 1 4 3
3 4 4 4
4 4 4 4
5 4 4 4
6 3 4 5
7 4 2 4
8 3 3 3
9 4 4 4
10 4 4 3
11 3 4 4
12 4 4 4
13 4 4 4
14 3 3 3
15 5 4 5
16 4 4 4
17 3 4 4
18 4 4 4
19 4 3 4
20 3 4 4
21 4 3 3
22 5 5 5
23 4 4 4
24 4 5 4
25 4 4 4
26 5 5 5
27 4 3 4
28 3 4 3
29 4 4 4
30 5 5 5
Rata-rata 3.80 3.90 3.97
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
FPM 30 3.8000 0.80516 1.00 5.00
FG 30 3.9000 0.66176 2.00 5.00
FCPMG 30 3.9667 0.61495 3.00 5.00
Friedman Test
Ranks
Mean Rank
FPM 1.92
FG 2.02
FCPMG 2.07
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 1.024 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.599
a. Friedman Test
Test Statisticsc
FG - FPM FPM - FCPMG FCPMG FG
Z -.504a -1.406b -.577a
Asymp. Sig. (2-tailed) 0.614 0.160 0.564
a. Based on negative ranks.
b. Based on positive ranks.
c. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 3 3 3
2 1 4 2
3 3 3 4
4 4 4 3
5 4 4 3
6 3 4 3
7 3 3 3
8 2 1 2
9 3 2 3
10 2 3 2
11 2 2 3
12 3 3 3
13 4 4 4
14 3 3 3
15 4 4 4
16 4 4 3
17 4 4 4
18 4 4 4
19 3 3 4
20 2 3 3
21 3 3 3
22 4 4 5
23 4 4 4
24 3 4 3
25 5 4 4
26 5 5 5
27 3 3 3
28 3 4 2
29 4 4 4
30 4 4 5
Rata-rata 3.30 3.47 3.37
b. Uji Friedman untuk parameter mudah menyebar hand and body cream
125
Descriptive Statistics
N Minimum Maximum Mean Std. Deviation
FPM 30 1.00 5.00 3.3000 0.91539
FG 30 1.00 5.00 3.4667 0.81931
FCPMG 30 2.00 5.00 3.3667 0.85029
Friedman Test
Ranks
Mean Rank
FPM 1.92
FG 2.07
FCPMG 2.02
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 0.792 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.673
a. Friedman Test
Test Statisticsc
FG - FPM FPM - FCPMG FCPMG - FG
Z -1.155a -.577b -.677b
Asymp. Sig. (2-tailed) 0.248 0.564 0.499
a. Based on negative ranks.
b. Based on positive ranks.
c. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 3 3 3
2 1 4 2
3 2 2 3
4 4 4 3
5 4 4 3
6 3 2 3
7 3 4 2
8 1 2 2
9 3 3 2
10 2 3 3
11 2 2 3
12 4 4 4
13 3 3 3
14 3 3 3
15 3 3 3
16 4 4 4
17 3 4 4
18 3 3 4
19 4 4 3
20 3 4 3
21 4 4 3
22 4 5 5
23 3 3 3
24 3 4 3
25 5 3 4
26 4 4 4
27 4 3 4
28 3 4 2
29 4 4 4
30 5 4 5
Rata-rata 3.23 3.43 3.23
b. Uji Friedman untuk parameter mudah menyerap hand and body cream
128
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
FPM 30 3.2333 0.97143 1.00 5.00
FG 30 3.4333 0.77385 2.00 5.00
FCPMG 30 3.2333 0.81720 2.00 5.00
Friedman Test
Ranks
Mean Rank
FPM 1.92
FG 2.13
FCPMG 1.95
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 1.463 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.481
a. Friedman Test
Test Statisticsd
FG - FPM FPM - FCPMG FCPMG - FG
Z -1.164a 0.000b -1.217c
Asymp. Sig. (2-tailed) .244 1.000 0.224
a. Based on negative ranks.
b. The sum of negative ranks equals the sum of positive ranks.
c. Based on positive ranks.
d. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 3 3 3
2 1 5 2
3 3 4 3
4 4 4 4
5 4 4 4
6 4 3 4
7 4 3 4
8 4 4 2
9 3 3 2
10 3 3 2
11 3 4 5
12 5 5 5
13 4 4 4
14 4 4 4
15 5 4 5
16 4 4 4
17 3 3 3
18 4 4 4
19 4 4 4
20 2 3 3
21 4 3 4
22 5 5 5
23 4 4 4
24 4 4 4
25 4 4 4
26 4 4 4
27 4 4 4
28 4 4 4
29 4 4 4
30 4 4 5
Rata-rata 3.73 3.83 3.77
b. Uji Friedman untuk parameter kesan lembut hand and body cream
131
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
FPM 30 3.7333 0.82768 1.00 5.00
FG 30 3.8333 0.59209 3.00 5.00
FCPMG 30 3.7667 0.89763 2.00 5.00
Friedman Test
Ranks
Mean Rank
FPM 1.98
FG 1.98
FCPMG 2.03
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 0.158 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.924
a. Friedman Test
Test Statisticsc
FG - FPM FPM - FCPMG FCPMG - FG
Z -.302a -.264b -.284b
Asymp. Sig. (2-tailed) .763 .792 .776
a. Based on negative ranks.
b. Based on positive ranks.
c. Wilcoxon Signed Ranks Test
Perlakuan
Panelis
FPM FG FCPMG
1 3 3 3
2 1 5 3
3 5 3 3
4 4 4 5
5 3 3 3
6 3 4 3
7 3 3 4
8 2 2 2
9 2 2 3
10 3 3 2
11 3 4 5
12 5 5 5
13 3 3 3
14 4 4 4
15 3 4 3
16 3 3 3
17 4 3 3
18 3 3 3
19 4 4 3
20 3 3 3
21 3 3 3
22 3 3 3
23 3 3 3
24 3 2 3
25 4 4 4
26 4 4 4
27 4 2 3
28 3 4 2
29 4 4 4
30 3 4 4
Rata-rata 3.27 3.37 3.30
b. Uji Friedman untuk parameter kesan lengket hand and body cream
134
Descriptive Statistics
N Mean Std. Deviation Minimum Maximum
FPM 30 3.2667 0.82768 1.00 5.00
FG 30 3.3667 0.80872 2.00 5.00
FCPMG 30 3.3000 0.79438 2.00 5.00
Friedman Test
Ranks
Mean Rank
FPM 1.97
FG 2.03
FCPMG 2.00
Test Statisticsa
N 30 Kesimpulan:
Chi-Square 0.163 Karena nilai Sig. > 0.05
Df 2 maka hasil dikatakan
tidak berbeda nyata
Asymp. Sig. 0.922
a. Friedman Test
Test Statisticsc
FG - FPM FPM - FCPMG FCPMG - FG
Z -.265a -.247b -.504b
Asymp. Sig. (2-tailed) 0.791 0.805 0.614
a. Based on negative ranks.
b. Based on positive ranks.
c. Wilcoxon Signed Ranks Test
136