MODUL PRAKTIKUM HEMATOLOGI

PEMERIKSAAN HEMATOKRIT

Tujuan :
Untuk dapat mengetahui prosedur pengukuran kadar hematokrit (Hct) atau Packed Cell Volume (PCV)
Prinsip :
Darah antikoagulan disentrifugasikan dalam jangka waktu dan kecepatan putar tertentu, sehingga sel –
selnya terpisah dalam keadaan mampat atau memadat. Presentase volume mampatan sel terhadap volume darah
semula dicatat sebagai hasil pemeriksaan hematokrit atau Packed Cell Volume (PCV)
Alat dan Bahan :
a) Darah antikoagulan
b) Tabung mikrohematokrit
c) Adonan wax setengah padat / malam
d) Sentrifuge mikrohematokrit
e) Tabung wintrobe
f) Sentrifuge tabung wintrobe / 2500 rpm
g) Sapu ijuk
h) Timer

Prosedur Kerja :
Metode pengukuran hematokrit secara manual dikenal ada 2, yaitu :
1. Metode makrohematokrit
Pada metode makro, sebanyak 1 ml sampel darah (darah EDTA atau heparin) dimasukkan dalam tabung
Wintrobe yang berukuran panjang 110 mm dengan diameter 2.5-3.0 mm dan berskala 0-10 mm. Tabung
kemudian disentrifus selama 30 menit dengan kecepatan 3.000 rpm. Tinggi kolom eritrosit adalah nilai
hematokrit yang dinyatakan dalam %.

2. Metode mikrohematokrit
Pada metode mikro, sampel darah (darah kapiler, darah EDTA, darah heparin atau darah amonium-kalium-
oksalat) dimasukkan dalam tabung kapiler yang mempunyai ukuran panjang 75 mm dengan diameter 1 mm.
Tabung kapiler yang digunakan ada 2 macam, yaitu yang berisi heparin (bertanda merah) untuk sampel
darah kapiler (langsung), dan yang tanpa antikoagulan (bertanda biru) untuk darah EDTA/heparin/amonium-
kalium-oksalat.

Hasil Pengamatan :
Nama Pasien :
Umur :
Jenis Kelamin :
Hasil Pemeriksaan :
Kesimpulan :

Nilai Normal :
 Bayi baru lahir : 50 – 62 %
 Anak 1 tahun : 31 – 39 %
 Dewasa wanita : 36 – 46 %

DP (2018)
MODUL PRAKTIKUM HEMATOLOGI

 Dewasa pria : 42 – 52 %

Catatan :
Hematokrit atau volume eritrosit yang dimampatkan (packed cell volume, PCV) adalah persentase volume
eritrosit dalam darah yang dimampatkan dengan cara diputar pada kecepatan tertentu dan dalam waktu tertentu.
Tujuan dilakukannya uji ini adalah untuk mengetahui konsentrasi eritrosit dalam darah. Pemeriksaan hematokrit
merupakan salah satu pemeriksaan darah khusus yang sering dikerjakan di laboratorium berguna untuk
membantu diagnosa berbagai penyakit diantaranya Demam Berdarah Dengue (DBD), anemia, polisitemia.

DP (2018)
 MODUL PRAKTIKUM HEMATOLOGI

LAPORAN HASIL PEMERIKSAAN

Hari / tanggal : .....................................................

Nama pasien : .....................................................
Jenis kelamin : .....................................................
Umur : .....................................................
Hasil Pemeriksaan dan Kesimpulan : .......................................................................................... ..................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
.................................................................................................................................................................... ....................................................
........................................................................................................................................................................................................... .............
..................................................................................................................... ...................................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
.............................................................................................................................................. ..........................................................................
..................................................................................................................................................................................... ...................................
............................................................................................... .........................................................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................................................... .........................................................
...................................................................................................................................................................................................... ..................
................................................................................................................ ........................................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
.................................................................................................................................................................... ....................................................
........................................................................................................................................................................................................... .............
..................................................................................................................... ...................................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
............................................................................................................................. ...........................................................................................
.............................................................................................................................................. ..........................................................................
..................................................................................................................................................................................... ...................................
DP (2018)
............................................................................................... .........................................................................................................................
............................................................................................................................. ...........................................................................................