DAFTAR ISI

JUDUL ............................................................................................................... i
PERNYATAAN ORISINALITAS dan PENYERAHAN HAK CIPTA ...... ii
PENGESAHAN ...............................................................................................iii
PERSEMBAHAN ............................................................................................ iv
KATA PENGANTAR ...................................................................................... v
ABSTRAK ...................................................................................................... vii
ABSTRACT ................................................................................................... viii
DAFTAR ISI ................................................................................................... ix
DAFTAR TABEL ........................................................................................... xi
DAFTAR GAMBAR ...................................................................................... xii
DAFTAR LAMPIRAN ................................................................................. xiii
BAB I. PENDAHULUAN ................................................................................. 1
1.1 Latar Belakang .............................................................................................. 1
1.2 Perumusan Masalah Penelitian .................................................................... 3
1.3 Tujuan Penelitian ......................................................................................... 3
1.4 Manfaat Penelitian ........................................................................................ 3
BAB II. TINJAUAN PUSTAKA ..................................................................... 5
2.1 Papain ............................................................................................................ 5
2.1.1 Manfaat Papain ............................................................................ 5
2.1.2 Faktor-Faktor Yang Mempengaruhi Kualitas Papain .................. 7
2.2.Mikroenkapsulasi ......................................................................................... 9
2.2.1 Definisi Mikroenkapsulasi dan Mikrokapsul .............................. 9
2.2.2 Tujuan Mikroenkapsulasi .......................................................... 10
2.2.3 Keuntungan Mikroenkapsulasi ................................................. 10
2.2.4 Prinsip Dasar Mikroenkapsulasi ............................................... 11
2.2.5 Klasifikasi Teknik Mikroenkapsulasi ....................................... 11
2.2.6 Teknik Mikroenkapsulasi .......................................................... 12
2.3 Eudragit RL 100 ......................................................................................... 17
BAB III. METODE PENELITIAN .............................................................. 19
3.1 Waktu dan Tempat Penelitian .................................................................... 19
3.2 Alat dan Bahan ........................................................................................... 19
3.2.1 Alat .......................................................................................... 19
3.2.2 Bahan ...................................................................................... 19
3.3 Pembuatan mikrokapsul ............................................................................. 19
3.4 Evaluasi dan karakterisasi mikrokapsul ..................................................... 20
3.4.1 Perolehan Kembali .................................................................... 20
3.4.2 Pemeriksaan Organoleptis ........................................................ 20
3.4.3 Penentuan Bentuk dan Morfologi Mikrokapsul ........................ 21
3.4.4 Penentuan Distribusi Ukuran Partikel ....................................... 21
3.4.5 Penentuan Kadar Air ................................................................. 21
3.4.6 Penentuan Panjang Gelombang Maksimum Larutan BSA
6,6 % dengan Spektrofotometer UV-Visible ............................ 21
3.4.7 Pembuatan Kurva Kalibrasi ...................................................... 22
3.4.8 Penetapan Kadar Protein Total Dari Mikrokapsul .................... 23
3.5 Analisis Data .............................................................................................. 23

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BAB IV. HASIL DAN PEMBAHASAN ....................................................... 24
4.1 Hasil ............................................................................................................ 24
4.2 Pembahasan ................................................................................................ 25
BAB V. KESIMPULAN DAN SARAN ......................................................... 33
5.1 Kesimpulan .......................................................................................... 33
5.2 Saran .................................................................................................... 33
DAFTAR PUSTAKA ..................................................................................... 34
LAMPIRAN

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 DAFTAR TABEL

Tabel Halaman
1. Proses Mikroenkapsulasi dan Penerapannya .......................................... 10
2. Klasifikasi Proses Mikroenkapsulasi ...................................................... 12
3. Formula Mikrokapsul Papain ................................................................. 20
4. Pembuatan Kurva Standar Bovin Serum Albumin ................................. 23
5. Hasil Pemeriksaan Organoleptis ............................................................. 41
6. Hasil Pengukuran Distribusi Ukuran Partikel Papain............................. 44
7. Hasil Pengukuran Distribusi Ukuran Partikel Mikrokapsul Papain
Formula 1 ................................................................................................ 45
8. Hasil Pengukuran Distribusi Ukuran Partikel Mikrokapsul Papain
Formula 2 ................................................................................................ 46
9. Hasil Pengukuran Distribusi Ukuran Partikel Mikrokapsul Papain
Formula 3 ................................................................................................ 47
10. Deret Standar .......................................................................................... 51
11. Hasil Penentuan Kadar Protein Pada Mikrokapsul Papain ..................... 52

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 DAFTAR GAMBAR

Gambar Halaman
1. Struktur Kimia Eudragit RL 100 ............................................................. 17
2. Morfologi Eudragit RL 100..................................................................... 19
3. Reaksi Antara Ikatan Peptida Dengan Logam Cu ................................... 30
4. Sertifikat Bahan Baku Papain ................................................................. 38
5. Sertifikatbahan Baku Eudragit RL 100 .................................................. 39
6. Mikrokapsul Papain................................................................................. 41
7. Morfologi Papain..................................................................................... 42
8. Morfologi Mikrokapsul Papain F1 .......................................................... 42
9. Morfologi Mikrokapsul Papain F2 .......................................................... 43
10. Morfologi Mikrokapsul Papain F3 .......................................................... 43
11. Kurva Distribusi Ukuran Partikel Papain ................................................ 48
12. Kurva Distribusi Ukuran Partikel Mikrokapsul Papain F1 ..................... 48
13. Kurva Distribusi Ukuran Partikel Mikrokapsul Papain F2 ..................... 49
14. Kurva Distribusi Ukuran Partikel Mikrokapsul Papain F3 ..................... 49
15. Spektrum Panjang Gelombang Serapan Maksimum ............................... 50
16. Kurva Kalibrasi Deret Standar Protein .................................................... 51
17. Homogenizer ........................................................................................... 54
18. Mouisture Balance................................................................................... 55
19. Optilab Microscope Camera.................................................................... 56
20. Spektrofotometer UV-Visible ................................................................. 57

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 DAFTAR LAMPIRAN

Lampiran Halaman
1. Skema Kerja Pembuatan Mikrokapsul Papain ....................................... 37
2. Sertifikat Bahan Baku ............................................................................. 38
3. Perolehana Kembali Mikrokapsul .......................................................... 40
4. Pemeriksaan Organoleptis ...................................................................... 41
5. Analisa Scanning Electron Microscopy (SEM) ..................................... 42
6. Distribusi Dan Ukuran Partikel .............................................................. 44
7. Spektrum Panjang Gelombang Serapan Maksimum .............................. 50
8. Pembuatan Kurva Kalibrasi Larutan Standar Bovin Serum Albumin .... 51
9. Analisa Kadar Protein Pada Mikrokapsul Papain ................................... 52
10. Analisa Data ANOVA Satu Arah ........................................................... 53
11. Alat-Alat Yang Digunakan ..................................................................... 54

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