ABSTRAK

RATNAWATI YUNINGSIH. Aktivitas Antibakteri Ekstrak Daun Jawer Kotok

(Coleus scutellaroides (L.) Benth.). Dibimbing oleh MARIA BINTANG dan
EMAN KUSTAMAN.
Penelitian ini mempelajari aktivitas antibakteri dan Konsentrasi Hambat
Tumbuh Minimum (KHTM) dari ekstrak daun jawer kotok terhadap bakteri Gram
positif (Staphylococcus aureus & Bacillus subtilis) dan bakteri Gram negatif
(Escherchia coli & Pseudomonas aeruginosa), serta penentuan senyawa metabolit
pada jawer kotok. Filtrat daun muda dan tua diuji aktivitas antibakterinya.
Daun jawer kotok tua secara umum memiliki aktivitas antibakteri yang lebih
besar. Daun jawer kotok tua diekstraksi menggunakan tiga pelarut, yaitu heksana,
air, dan aseton. Ekstrak daun jawer kotok diuji aktivitas antibakterinya. Ekstrak
aseton memiliki zona hambat paling besar terhadap bakteri uji. Ampisilin 0.4
mg/mL digunakan sebagai kontrol positif. Uji kualitatif fitokimia ekstrak aseton
menunjukkan hasil positif untuk uji senyawa alkaloid dan steroid.
Zona hambat yang dihasilkan memiliki korelasi positif dengan konsentrasi
ekstrak daun jawer kotok. Konsentrasi Hambat Tumbuh Minimum ekstrak daun
jawer kotok terhadap bakteri B. Subtilis, S. aureus, E. coli, dan P. aeruginosa
adalah 0,1 mg/mL dengan zona hambat berturut-turut adalah 6.6438, 6.5, 6.8062,
dan 6.6188 mm. Zona hambat ampisilin 0.4 mg/mL terhadap B. subtilis, S. aureus,
E. coli, dan P. aeruginosa masing-masing sebesar 26.2, 25.6042, 24.7708, dan
25.5292 mm.
.
 ABSTRACT

RATNAWATI YUNINGSIH. Antibacterial Activity from Extract of Jawer Kotok

(Coleus scutellaroides [L] Benth.) Leaves. Under the supervisor MARIA
BINTANG dan EMAN KUSTAMAN.
This research studied antibacterial activity and Minimum Inhibitory
Concentration (MIC) from jawer kotok leaves extract to positive Gram bacteria
(Staphylococcus aureus & Bacillus subtilis) and negative Gram bacteria
(Escherchia coli & Pseudomonas aeruginosa), and also qualitative determination
of secondary metabolite from jawer kotok. Antibacterial activity from old and
young leaves filtrate were tested.
Old leaves generally have higher antibacterial activity. Old jawer kotok
leaves extracted using 3 solvent, hexane, water, and acetone. Antibacterial activity
from leaves extract was tested. Acetone extract has the biggest inhibitory zone
after testified at those bacteria. Ampicillin 0.4 mg/mL is used as positive control.
Phytochemical test to acetone extract showed positive result for alkaloid and
steroid.
The resulted inhibition zone has a positive correlation with the leaves
exctract concentration. Minimum Inhibitory Concentration (MIC) of jawer kotok
leaves extract to B. Subtilis, S. aureus, E. coli, and P. aeruginosa are 0.1 mg/mL
with inhibitory zone 6.6438, 6.5000, 6.8062, and 6.6188 mm respectively.
Ampicillin hambat zone (0.4 mg/mL) to B. subtilis, S. aureus, E. coli, and P.
aeruginosa are 26.2, 25.6042, 24.7708, and 25.5292 mm respectively