ABSTRAK

Daun binahong (Anredera cordifolia) merupakan salah satu tanaman obat tradisional
yang memiliki aktivitas antibakteri. Tanaman ini mengandung senyawa metabolit sekunder
seperti saponin, triterpenoid, alkaloid, polifenol, kuinon yang dapat menghambat bakteri. Bakteri
Staphylococcus epidermidis merupakan bakteri yang terdapat pada kulit serta menyebabkan
infeksi pada pasien imunokompeten saat integritas penghalang kulit terganggu. Staphylococcus
epidermidis merupakan bakteri oportunistik yang hanya dapat menyebabkan infeksi pada pasien
dengan kondisi imunosupresi, kondisi medis seperti infeksi HIV, (faktor predisposisi seperti lahir
prematur, cacat imun bawaan), setelah transplantasi organ padat dan kemoterapi terkait
neutropenia. Penelitian ini bertujuan untuk mengetahui daya hambat daun binahong terhadap
bakteri Staphyloccus epidermidis. Ekstrak daun binahong diperoleh dengan cara maserasi
menggunakan etanol 96%. Perlakuan terdiri dari ekstrak etanol daun binahong dengan
konsentrasi 25%, 50%, 75%. Amoxicilin dan aquadest masing-masing digunakan sebagai kontrol
positif dan kontrol negatif. Hasil penelitian menunjukan bahwa ekstrak etanol daun binahong
konsentrasi 25%, 50%, dan 75% menghasilkan zona hambat rata-rata terhadap pertumbuhan
Staphylococcus epidermidis masing-masing sebesar 6 mm, 6,33 mm, 7 mm. Hasil analisis data
diperoleh bahwa ekstrak etanol daun binahong dalam menghambat pertumbuhan Staphylococcus
epidermidis terdapat zona hambat pada konsentrasi 75%, namun pada konsentrasi 25% dan 50%
tidak terdapatnya zona hambat pertumbuhan Staphylococcus epidermidis.

Kata Kunci: Tanaman binahong (Anredera cordifolia), Staphylococcus epidermidis, daya

hambat.

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 ABSTRACT

Binahong leaves (Anredera cordifolia) is one of the traditional medicinal plants that have
antibacterial activity. This plant contains secondary metabolite compounds such as saponins,
triterpenoids, alkaloids, polyphenols, quinones that can inhibit bacteria. Bacteria Staphylococcus
epidermidis is a bacteria found in the skin and causes infection in immunocompetent patients
when the integrity of the skin barrier is impaired. Staphylococcus epidermidis is an opportunistic
bacteria that can only cause infection in patients with immunosuppressed conditions, medical
conditions such as HIV infection, (predisposing factors such as premature birth, congenital
immune defects), after solid organ transplants and chemotherapy-related neutropenia. This study
aims to determine the inhibitability of the leaves of binahong against Staphyloccus epidermidis
bacteria. The leaf extract of binahong was obtained by maceration using 96% ethanol. The
treatment consisted of ethanol extract of binahong leaf with concentration 25%, 50%, 75%.
Amoxicillin and aquadest are each used as positive controls and negative controls. The results
showed that the concentration of 25%, 50% and 75% ethanol leaves of binahong extract resulted
in an average inhibitory zone of Staphylococcus epidermidis growth of 6 mm, 6.33 mm, 7 mm,
respectively. The result of data analysis showed that extract of binahong leaf extract inhibiting
the growth of Staphylococcus epidermidis contain inhibition zone at 75% concentration, but at
concentration 25% and 50% there is no inhibition zone of Staphylococcus epidermidis growth.

Keywords: Binahong plants (Anredera cordifolia), Staphylococcus epidermidis, inhibitory

power.

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