Penetapan P metode Bray 1

Deret Standar Data Sampel

Konsentrasi (ppm) Absorbansi Ulangan bobot (gram)
0 -0.001 1 4.9998
0.25 0.006 2 5.0001
0.5 0.016 3 5
0.75 0.03 4 5
1 0.036 5 5.0004
1.5 0.055 6 4.9998
2 0.077 7 4.9995
4 0.158 8 5.0003
Korelasi r 0.999440062 9 5
intercept (a) -0.00290188 10 5.0002
Slope (b) 0.040021505 11 5
12 5
13 5.0003
14 5
15 4.9998

ESTIMASI KETIDAKPASTIAN

1. FISHBONE

U PM U reg U massa

Kadar P (mg/kg)

U PV 3 U PV 2 U PV1
PERHITUNGAN
1. Ketidakpastian Asal Kurva Kalibrasi
No. deret x (ppm) y yc (yi-yc)^2 (Xi-Xr)^2
1 0 -0.001 -0.002901882 3.617154E-06 4.93827160494
2 0.25 0.006 0.0071034946 1.2177E-06 3.88966049383
3 0.5 0.016 0.017108871 1.229595E-06 2.96604938272
4 0.75 0.03 0.0271142473 8.327569E-06 2.1674382716
5 1 0.036 0.0371196237 1.253557E-06 1.49382716049
6 1.5 0.055 0.0571303763 4.538503E-06 0.52160493827
7 2 0.077 0.077141129 1.99174E-08 0.04938271605
8 4 0.158 0.1571841398 6.656279E-07 3.16049382716
Jumlah 10 0.377 0.377 2.086962E-05 19.1867283951
Rerata 2.222222222 0.083778 0.0837777778 4.637694E-06 4.26371742112
n 8
n-2 6
yo 0.0043
Slope (b) 0.040021505
Intercept(a) -0.00290188
1+1/n 1.125
∑(Yi-Yc) 2.08696E-05
RSD atau Sy/x 0.001865012 0.1398007554
(Yo-Yr)^2 0.006316717 0.4660025179
b^2∑(Xi-Xr)^2 0.030731784
1+1/n+(yo-yr)^2/b^2∑(Xi-Xr)^2 1.33054346
√1+1/n+(yo-yr)^2/b^2∑(Xi-Xr)^2 1.153491855
U reg 0.053753011

Ketidakpastian Asal Neraca

Neraca Analitik
Kalibrasi spekk (95%) Ukal U massa
U kal 0.1 2 0.05 0.070710678

Ketidakpastian Asal Pipet Volumetri

Pipet Volumetri
UT Koef muai Volume Var. suhu k UT
0.00021 25 8 1.732050808 0.02424871131
Kalibrasi spek k u Kal
0.1 1.73205080756888 0.057735026918963

Pipet Volumetri
UT Koef muai Volume Var. suhu k UT
0.00021 10 8 1.732050808 0.00969948452
Kalibrasi spek k u Kal
 0.1 1.73205080756888 0.057735026918963

Pipet Volumetri
UT Koef muai Volume Var. suhu k UT
0.00021 2 8 1.732050808 0.0019398969
Kalibrasi spek k u Kal
0.01 1.73205080756888 0.005773502691896

Ketidakpastian Gabungan
Asal ketidakpastian satuan xi Uxi (Uxi/xi)^2
Kurva Kalibrasi mg/L 0.17995 0.0537530109 0.089227857
Presisi Metode mg/kg 4.68 0.44 0.008787383
Neraca mg 5000.05 0.0707106781 1.99996E-10
Pipet Volumetri 25 mL mL 25 0.0626205504 6.274133E-06
Pipet Volumetri 10 mL mL 10 0.0585441144 3.427413E-05
Pipet Volumetri 2 mL mL 2 0.0060906924 9.274133E-06
∑(Uxi/xi)^2 0.098065062
Uxi/xi 0.313153417
Uxi 1.46561624
U diperluas 2.93123248

Pelaporan
Kadar P tersedia dalam sampel Tanah
4,68 ± 3,00 mg/kg

Ulangan abs c terukur

1 0.0080.256041
2 0.0080.256041
3 0.0080.256041
4 0.0110.330125
5 0.0090.280736
6 0.01 0.30543
7 0.0110.330125
rerata 0.287791
SD 0.034082
3SD 0.102246
LOD teoritis 0.139801

Ulangan abs c terukur

1 0.023
2 0.024
3 0.024
4 0.023
5 0.025
6 0.024
7 0.025
 Data Sampel Setelah Seleksi Data
absorbansi ppm kurva kadar P (ppm) Ulangan bobot (gram) absorbansi
0.002 0.122481193 3.18566986295 5 5.0004 0.003
0.006 0.222427458 5.78487129316 7 4.9995 0.004
0.002 0.122481193 3.18554243615 8 5.0003 0.004
0.008 0.272400591 7.0847092678 9 5 0.005
0.003 0.147467759 3.83509676702 10 5.0002 0.004
0.002 0.122481193 3.18566986295 11 5 0.005
0.004 0.172454326 4.4857132847 12 5 0.004
0.004 0.172454326 4.48499561363 13 5.0003 0.004
0.005 0.197440892 5.13512585198 14 5 0.005
0.004 0.172454326 4.48508530996 15 4.9998 0.005
0.005 0.197440892 5.13512585198 Rerata 5.00005 0.0043
0.004 0.172454326 4.48526471337
0.004 0.172454326 4.48499561363
0.005 0.197440892 5.13512585198
0.005 0.197440892 5.13533126523
Rerata 4.6152215231
SD 1.04786152892
RSD 22.7044687601
Batas atas 5.40813823837
Batas Bawah 3.82230480783
 U PV 25 mL
0.0626205504

U PV 10 mL
0.0585441144
0.0585441144

U PV 2 mL
0.0060906924
ppm kurva kadar P (ppm)
0.14746775927 3.84
0.17245432563 4.49
0.17245432563 4.48
0.19744089199 5.14
0.17245432563 4.49
0.19744089199 5.14
0.17245432563 4.49
0.17245432563 4.48
0.19744089199 5.14
0.19744089199 5.14
0.17995029554 4.68
SD atau U PM 0.44
RSD 9.37
2/3*CV Horwitz 10.67
KETERANGAN Memenuhi syarat RSD <
2/3 CV Horwitz
Rerata ppm kurv 0.179950295540032
Data Deret standar

konsentrasAbsorbansi
0 -0.0001
0.25 0.01
0.5 0.024
0.75 0.03
1 0.041
1.5 0.056
2 0.078
4 0.154
r 0.999272
a 0.001492
b 0.038097

Data Pengukuran Sampel

Ulangan Bobot sampAbsorbansiC terukur C sampel+Spike C sampel C spike Recovery Cterukur sampel
1 5.0004 0.013 0.302085 9.4273534992 4.68 1 12.21348 0.17995
2 5.0004 0.012 0.275836 8.608186803 4.68 1 392.8187 0.17995
3 5.0002 0.012 0.275836 8.6085311167 4.68 1 392.8531 0.17995
4 4.9998 0.012 0.275836 8.6092198267 4.68 1 392.922 0.17995
5 5.0002 0.012 0.275836 8.6085311167 4.68 1 392.8531 0.17995
6 5.0006 0.01 0.223338 6.9695746497 4.68 1 228.9575 0.17995
7 5.0004 0.008 0.17084 5.3315200179 4.68 1 65.152 0.17995
9 4.9995 0.012 0.275836 8.6097364316 4.68 1 392.9736 0.17995
10 5.0003 0.007 0.144591 4.5124435633 4.68 1 -16.75564 0.17995

Data Standar Data Sampel

konsentrasAbsorbansi Ulangan bobot abs c terukur C sampel+sc sampel t.
0 0.001 1 4.9995 0.043 1.120353 33.99845 4.68
0.25 0.01 2 5.0002 0.045 1.169742 35.49226 4.68
0.5 0.017 3 5.0001 0.044 1.145048 34.74367 4.68
0.75 0.027 4 5.0006 0.066 1.68833 51.22311 4.68
1 0.036 5 4.9995 0.069 1.762414 53.48255 4.68
1.5 0.056 6 5.0005 0.069 1.762414 53.47185 4.68
2 0.077 7 5.0004 0.104 2.626726 79.69679 4.68
4 0.162 8 5.0003 0.099 2.503253 75.95204 4.68
r 0.99904 9 5.0003 0.101 2.552642 77.45057 4.68
a -0.002368 10 4.9996 0.104 2.626726 79.70954 4.68
b 0.040495
0.116 Ulangan abs C terukur + SpiC terukur c spike Recovery
1 0.043 1.1203531599 0.17995 94.04029
2 0.045 1.1697424323 0.17995 98.97921
3 0.044 1.1450477961 0.17995 1 96.50975
4 0.066 1.6883297929 0.17995 75.41897
5 0.069 1.7624137015 0.17995 79.12317
6 0.069 1.7624137015 0.17995 2 79.12317
7 0.104 2.6267259692 0.17995 81.55919
8 0.099 2.5032527881 0.17995 77.44342
9 0.101 2.5526420605 0.17995 79.08973
10 0.104 2.6267259692 0.17995 3 81.5592
Rerata 84.28461
Memenuhi syarat rentang (75-

Penentuan LOD dan LOQ

Ulangan Absorbansi C terukur (mg/L)
1 0.002 0.1078730749
2 0.002 0.1078730749
3 0.003 0.1325677111
4 0.003 0.1325677111
5 0.001 0.0831784387
6 0.003 0.1325677111
7 0.004 0.1572623473
8 0.003 0.1325677111
9 0.003 0.1325677111
10 0.003 0.1325677111
Rerata 0.1251593202
SD 0.0203304174
3SD 0.0609912523
10SD 0.2033041742
LOD 0.1861505725
LOQ 0.3284634945
0.002368
Cterukur sampel

c spike Recovery Spike 1 Spike 2 Spike 3

28.74986 101.9777 94.04029 28.74986 59.4717 88.69492
28.74986 107.1736 98.97921
28.74986 104.5698 96.50975
59.4717 78.26094 75.41897
59.4717 82.06012 79.12317
59.4717 82.04213 79.12317
88.69492 84.57845 81.55919
88.69492 80.35639 77.44342
88.69492 82.04593 85.08807
88.69492 84.59282 87.55753
Memenuhi syarat rentang (75-110%) menurut AOAC guidelines