ABSTRAK

FORMULASI DAN UJI KARAKTERISTIK FISIK SEDIAAN

MIKROEMULSI GEL VITAMIN E (α-tocopherol) SEBAGAI
ANTIOKSIDAN DENGAN GELLING AGENT HPMC K100M
(Variasi kadar gelling agent HPMC K100M 1%, 2%, 3%)

Alena Putri Jathy, Uswatun Chasanah, Raditya Weka Nugraheni

Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah
Malang

Latar Belakang: Radikal bebas dalam jumlah berlebih mengakibatkan stress

oksidatif yang dapat menyebabkan kerusakan mulai dari tingkat sel, jaringan,
hingga ke organ tubuh yang mempercepat terjadinya proses penuaan dan
munculnya penyakit. Vitamin E merupakan senyawa antioksidan yang dapat
mencegah kerusakan DNA dan melindungi membran sel dari radikal bebas. Telah
dilakukan penelitian formulasi sediaan mikroemulsi gel vitamin E dengan gelling
agent HPMC K100M.
Tujuan: Mengetahui pengaruh variasi kadar gelling agent HPMC K100M
terhadap karakteristik fisik mikroemulsi antioksidan Vitamin E.
Metode: Uji aktivitas antioksidan menggunakan metodeh DPPH, Analisis
dilakukan secara deskriptif (organoleptis, tipe mikroemulsi, homogenitas, dan
stabilitas Freeze-Thaw) dan uji statistik One-Way Annova (viskositas, daya sebar,
dan pH).
Hasil dan Kesimpulan: Mikroemulsi Vitamin E (a-tocopherol) merupakan
sediaan dengan tipe M/A. Vitamin E (a-tocopherol) memiliki nilai IC50 sebesar
133,71 ppm yang dikategorikan sebagai antioksidan sedang. Aktifitas antioksidan
sediaan mikroemulsi vitamin E sejumlah 250 ppm memiliki % Inhibitor 68,2 %.
Semua formula mikroemulsi gel memiliki tekstur yang lembut, berwarna putih
kekuningan dan berbau khas vitamin E. Hasil uji statistik One-Way Annova
didapatkan ketiga formula memiliki perbedaan bermakna untuk viskositas, daya
sebar, dan pH. Formula dengan konsentrasi HPMC K100M 3% (FIII) adalah
formula terbaik karena masuk lebih stabil dibandingkan dengan formula lainnya.

Kata Kunci: Vitamin E (a-tocopherol), Antioksidan, Mikroemulsi Gel, HPMC

K100M
 ABSTRACT

FORMULATION AND PHYSICAL CHARACTERISTICS OF

VITAMIN E (α-tocopherol) GEL MICROEMULSION AS
ANTIXOXIDE WITH GELLING AGENT HPMC K100M
(Variation in levels of gelling agent HPMC K100M 1%, 2%, 3%)

Alena Putri Jathy, Uswatun Chasanah, Raditya Weka Nugraheni

Pharmacy Study Program, Faculty of Health Sciences, Muhammadiyah University
of Malang

Background: Excessive amounts of free radicals cause oxidative stress which can
cause damage of cellular, tissues, and organs of the body which accelerate the
aging process and the diseases. Vitamin E is an antioxidant compound that can
prevent DNA damage and protect cell membranes from free radicals. A research
on the formulation of vitamin E gel microemulsion gel formulation with HPMC
K100M gelling agent has been carried out.
Objective: To determine the effect of variations in the level of gelling agent
HPMC K100M on the physical characteristics of antioxidant microemulsion
Vitamin E.
Method: Antioxidant activity test using the DPPH method, the analysis was done
descriptively (organoleptic, microemulsion type, homogeneity, and Freeze-Thaw
stability) and One-Way Annova statistical test (viscosity, dispersion, and pH).
Results and Conclusions: Vitamin E (a-tocopherol) microemulsion is a type
O/W preparation. Vitamin E (a-tocopherol) has an IC50 value of 133.71 ppm
which is categorized as a moderate antioxidant. The antioxidant activity of
vitamin E microemulsion preparations in the amount of 250 ppm has an %
Inhibitors of 68.2%. All gel microemulsion formulas have a soft, yellowish-white
texture and smell typical of vitamin E. The One-Way Annova statistical test
results found that the three formulas have significant differences for viscosity,
spreadability, and pH. Formula with a concentration of HPMC K100M 3% (FIII)
is the best formula because it enters more stable compared to other formulas.

Keywords: Vitamin E (a-tocopherol), Antioxidants, Gel Microemulsion, HPMC

K100M