LAPORAN PRAKTIKUM

GENETIKA TUMBUHAN

Semester:
Ganjil 2014

Oleh:
Anisa Margareta S
A1L013076/ C

KEMENTERIAN PENDIDIKAN DAN KEBUDAYAAN

UNIVERSITAS JENDERAL SOEDIRMAN
FAKULTAS PERTANIAN
LABORATORIUM PEMULIAAN TANAMAN DAN BIOTEKNOLOGI
PURWOKERTO
2014

LAPORAN PRAKTIKUM
MATA KULIAH GENETIKA TUMBUHAN

Oleh:
Anisa Margareta S
(A1L013076/ C)

Diterima dan disetujui

Tanggal:

Asisten Praktikum,

Ricky Yuliawan Sigmatika

NIM A1L012149

PRAKATA

Puji syukur kepada Tuhan Yang Maha Esa penulis panjatkan atas rahmat-Nya dapat
menyelesaikan Laporan Praktikum Genetika Tumbuhan dengan lancar. Ilmu Genetika
merupakan ilmu yang sangat diperlukan untuk menemukan-menemukan varietas yang baru
yang memiliki keunggulan yang dapat memajukan pertanian di Indonesia. Oleh karena itu
untuk mempelajari genetik perlu dilakukan praktikum.
Penulisan laporan ini tidak lepas dari bantuan banyak pihak. Oleh karena itu penulis
mengucapkan terima kasih kepada Yth :
1. Dr.Ir. Anisur Rosyad, M.S.,selaku Dekan Fakultas Pertanian Universitas Jenderal
Soedirman.
2. Ir.Suprayogi, M. Sc., Ph. D., selaku Kepala Laboratorium Pemuliaan dan Bioteknologi.
3. Dr. Ir. Ponendi Hidayat, M.P., selaku Koordinator Praktikum Genetika Tumbuhan Semester
Ganjil tahun 2014.
4. Prita Sari Dewi. S.P., M. Sc., Ph. D., selaku Koordinator Praktikum Genetika Tumbuhan
Semester Ganjil tahun 2014.
5. Kedua orang tua, keluarga besar, dan sahabat yang
telah memberikan semangat dan bantuan sehingga dapat tersusnnya laporan
praktikum ini.
6. Semua asisten genetika tumbuhan yang bersedia meluangkan waktunya
7. Ricky Yuliawan selaku PJ laporan genetika tumbuhan
Penulis berharap agar laporan ini dapat berguna buat yang lainnya. Terima kasih.
Purwokerto, 12 Desember 2014
penulis

DAFTAR ISI

Halaman
HALAMAN JUDUL ..........................................................................................i
LEMBAR PENGESAHAN ...............................................................................ii
PRAKATA ..........................................................................................................iii
DAFTAR ISI.......................................................................................................iv
DAFTAR LAMPIRAN.......................................................................................v
1.ACARA 1. PENGAMATAN PERILAKU KROMOSOM..............................1
I. PENDAHULUAN...........................................................................................2
A. Latar Belakang..............................................................................................2
B.

Tujuan....................................................................................................3

II.TINJAUAN PUSTAKA..................................................................................4
III.METODE PRAKTIKUM..............................................................................6
A.Bahan dan Alat................................................................................................6
B.Prosedur Kerja.................................................................................................6
IV.HASIL DAN PEMBAHASAN......................................................................8
A.Hasil................................................................................................................8
B.Pembahasan.....................................................................................................9
V.KESIMPULAN DAN SARAN.......................................................................18
A.Kesimpulan.....................................................................................................18
B.Saran................................................................................................................18
DAFTAR PUSTAKA..........................................................................................19
LAMPIRAN........................................................................................................20
2.ACARA II. TEORI KEMUNGKINAN...........................................................21
I. PENDAHULUAN...........................................................................................22
A. Latar Belakang..............................................................................................22
B. Tujuan...........................................................................................................23

II.TINJAUAN PUSTAKA..................................................................................24
III.METODE PRAKTIKUM..............................................................................26
A.Bahan dan Alat................................................................................................26
B.Prosedur Kerja.................................................................................................26
IV.HASIL DAN PEMBAHASAN......................................................................27
A.Hasil................................................................................................................27
B.Pembahasan.....................................................................................................30
V.KESIMPULAN DAN SARAN.......................................................................36
A.Kesimpulan.....................................................................................................36
B.Saran................................................................................................................36
DAFTAR PUSTAKA..........................................................................................37
LAMPIRAN........................................................................................................38
3.ACARA 3. PERSILANGAN MONOHIBRID................................................39
I. PENDAHULUAN...........................................................................................40
A. Latar Belakang..............................................................................................40
B. Tujuan...........................................................................................................40
II.TINJAUAN PUSTAKA..................................................................................41
III.METODE PRAKTIKUM..............................................................................43
A.Bahan dan Alat................................................................................................43
B.Prosedur Kerja.................................................................................................43
IV.HASIL DAN PEMBAHASAN......................................................................44
A.Hasil................................................................................................................44
B.Pembahasan.....................................................................................................45
V.KESIMPULAN DAN SARAN.......................................................................51
A.Kesimpulan.....................................................................................................51
B.Saran................................................................................................................51
DAFTAR PUSTAKA..........................................................................................52
LAMPIRAN........................................................................................................53
4.ACARA IV PERSILANGAN DIHIBRID.......................................................54

I.PENDAHULUAN............................................................................................55
A. Latar Belakang..............................................................................................55
B.

Tujuan.........................................................................................................55

II.TINJAUAN PUSTAKA..................................................................................55
III.METODE PRAKTIKUM..............................................................................59
A.Bahan dan Alat................................................................................................59
B.Prosedur Kerja.................................................................................................59
IV.HASIL DAN PEMBAHASAN......................................................................61
A.Hasil................................................................................................................61
B.Pembahasan.....................................................................................................62
V.KESIMPULAN DAN SARAN.......................................................................69
A.Kesimpulan.....................................................................................................69
B.Saran................................................................................................................69
DAFTAR PUSTAKA..........................................................................................70
LAMPIRAN........................................................................................................71
5.ACARA V. PENYIMPANGAN HUKUM MENDEL.....................................72
I.PENDAHULUAN............................................................................................73
A. Latar Belakang..............................................................................................73
B. Tujuan...........................................................................................................74
II.TINJAUAN PUSTAKA..................................................................................75
III.METODE PRAKTIKUM..............................................................................77
A.Bahan dan Alat................................................................................................77
B.Prosedur Kerja.................................................................................................77
IV.HASIL DAN PEMBAHASAN......................................................................78
A.Hasil................................................................................................................78
B.Pembahasan.....................................................................................................85
V.KESIMPULAN DAN SARAN.......................................................................88
A.Kesimpulan......................................................................................................88
B.Saran................................................................................................................88

DAFTAR PUSTAKA..........................................................................................89
LAMPIRAN........................................................................................................90
6.ACARA VI. PERHITUNGAN FREKUENSI ALEL, FREKUENSI GENOTIP,
PENGUKURAN SIFAT-SIFAT KUALITATIF DAN KUANTITATIF..............101
I.PENDAHULUAN............................................................................................102
A. Latar Belakang..............................................................................................102
B. Tujuan...........................................................................................................102
II.TINJAUAN PUSTAKA..................................................................................104
III.METODE PRAKTIKUM..............................................................................106
A.Bahan dan Alat................................................................................................106
B.Prosedur Kerja.................................................................................................106
IV.HASIL DAN PEMBAHASAN......................................................................108
A.Hasil................................................................................................................108
B.Pembahasan.....................................................................................................112
V.KESIMPULAN DAN SARAN.......................................................................118
A.Kesimpulan......................................................................................................118
B.Saran................................................................................................................118
DAFTAR PUSTAKA..........................................................................................119
LAMPIRAN........................................................................................................120
BIODATA PENULIS..........................................................................................121

DAFTAR LAMPIRAN

Lampiran

Halaman

1.Acara I Pengamatan perilaku kromosom.........................................................20

2.Acara II Teori peluang......................................................................................38
3.Acara III Persilangan monohibrid....................................................................53
4.Acara IV Persilangan dihibrid..........................................................................71
5.Acara V Penyimpangan hukum Mendel..........................................................100
6Acara VI Perhitungan frekuensi allel, frekuensi genotip, pengukuran sifat-sifat kualitatif
dan kuantitatif.................................................................................................120